Autophagic induction modulates splenic plasmacytoid dendritic cell mediated immune response in cerebral malarial infection model

Sengupta, Anirban; Keswani, Tarun; Sarkar, Samrat; Ghosh, Soubhik; Mukherjee, Saikat; Bhattacharyya, Arindam

doi:10.1016/j.micinf.2019.05.004

Cited by 15 publications

(12 citation statements)

References 47 publications

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“…The Treg cell population is more tolerogenic, while Th17 shows a more aggressive response and drives the Th1 type of immune response [31], [37] The imbalance in Treg/Th17 has been reported in multiple diseases before [37]- [39]. A low Treg: Th17 ratio in anionic N3 groups is observed as compared to the L3 groups (Fig 3E ) indicating aggressive Th1 response in cationic treated groups.…”

Section: Higher Population Percentage Of Cd4t Cell With the Altered S...mentioning

confidence: 72%

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Anionic and Cationic Lipid Adjuvants individually induce distinct adaptive Th1/Th2-type immunity in enhancing post-intranasal immunization response against influenza

Sengupta

Al-Otaibi

Lottersberger

et al. 2022

Preprint

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At this time when vaccine development is at its peak against different respiratory diseases, it is of utmost importance to find suitable adjuvants that can increase the potency of the vaccine candidates. In this study, we have shown how anionic and cationic lipid adjuvants can differ in their mechanism to induce immune protection against influenza. In presence of Hemagglutinin (HA) antigen, the anionic adjuvant (L3) induces enhanced dendritic cell activity, CD80, and CD86 costimulatory marker expression, MHCII, and DEC205 expression, and T cell activation. On the contrary, the cationic adjuvant (N3) induces MHCI expression on dendritic cells along with the higher Th17 cell population and enhanced CD28 expression and activation of CD8T cells. They exhibited significantly higher interferon-gamma (IFNγ) within both CD4T and CD8T cells. L3 treated groups produce significantly higher B plasma cells and higher titers of anti-HA IgG and IgA with more neutralization capacity of the live virus than the N3 groups. Thus, in this study, we illustrate how the use of differentially charged lipid adjuvants in combination with influenza HA antigen, drives differential adaptive immune response patterns. While anionic adjuvants are inducing better humoral response than cationic adjuvants, the latter influence significantly higher cell-mediated immunity. This will pave the way forward in the selection of the adjuvants in the future development of vaccine formulation targeting specific groups of individuals having a deficit in one or the other arm of adaptive immunity.

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Section: Higher Population Percentage Of Cd4t Cell With the Altered S...mentioning

confidence: 72%

“…Flow cytometry on the splenic single cell suspension was done as described before [31], [32]. Euthanized mice were sacri ced on the 69th day post-infection and spleens were harvested out.…”

Section: Flow Cytometrymentioning

confidence: 99%

Anionic and Cationic Lipid Adjuvants individually induce distinct adaptive Th1/Th2-type immunity in enhancing post-intranasal immunization response against influenza

Sengupta

Al-Otaibi

Lottersberger

et al. 2022

Preprint

Self Cite

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“…Clearing blood-borne pathogens is a hallmark feature of the spleen. In this secondary lymphoid organ, pDCs and red pulp macrophages efficiently cleared malaria parasites after boosting autophagy with rapamycin (67,68). The treatment enhanced antigen presentation in these cells and shifted the cytokine and chemokines profile in vivo which recruited effector cells into the spleen and enhanced T cell responses.…”

Section: Therapeutic Approaches Against Infections By Modulating Automentioning

confidence: 98%

Targeting Autophagy in Innate Immune Cells: Angel or Demon During Infection and Vaccination?

Tao

Drexler

2020

Front. Immunol.

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Innate immune cells are the "doorkeepers" in the immune system and are important for the initiation of protective vaccine responses against infection. Being an essential regulatory component of the immune system in these cells, autophagy not only mediates pathogen clearance and cytokine production, but also balances the immune response by preventing harmful overreaction. Interestingly, recent literature indicates that autophagy is positively or negatively regulating the innate immune response in a cell type-specific manner. Moreover, autophagy serves as a bridge between innate and adaptive immunity. It is involved in antigen presentation by delivering pathogen compounds to B and T cells, which is important for effective immune protection. Upon infection, autophagy can also be hijacked by some pathogens for replication or evade host immune responses. As a result, autophagy seems like a double-edged sword to the immune response, strongly depending on the cell types involved and infection models used. In this review, the dual role of autophagy in regulating the immune system will be highlighted in various infection models with particular focus on dendritic cells, monocytes/macrophages and neutrophils. Targeting autophagy in these cells as for therapeutic application or prophylactic vaccination will be discussed considering both roles of autophagy, the "angel" enhancing innate immune responses, antigen presentation, pathogen clearance and dampening inflammation or the "demon" enabling viral replication and degrading innate immune components. A better understanding of this dual potential will help to utilize autophagy in innate immune cells in order to optimize vaccines or treatments against infectious diseases.

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“…The splenic plasmacytoid dendritic cells might act as a Trojan horse and carry the live Plasmodium within it. Rapamycin treatment reverses it by inducing an autophagic flux shift of the aggressive Th1 immune pathway, which is reported to incur host damage to a better well-balanced cytokine profile Th2 pathway (increase in IL10/TNF-α, regulatory T cell Treg/Th17) [ 73 ]. The IL-10 also has a role in cross-reactive memory response.…”

Section: Malaria and Inflammationmentioning

confidence: 99%

Mediterranean Diet: Lipids, Inflammation, and Malaria Infection

Silva

Moraes

Gonçalves-de-Albuquerque

2020

IJMS

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The Mediterranean diet (MedDiet) consists of consumption of vegetables and healthy oils and have beneficial effects on metabolic and inflammatory diseases. Our goal here is to discuss the role of fatty acid content in MedDiet, mostly omega-3, omega-6, and omega-9 on malaria. Malaria affects millions of people around the globe. The parasite Plasmodium causes the disease. The metabolic and inflammatory alterations in the severe forms have damaging consequences to the host. The lipid content in the MedDiet holds anti-inflammatory and pro-resolutive features in the host and have detrimental effects on the Plasmodium. The lipids from the diet impact the balance of pro- and anti-inflammation, thus, lipids intake from the diet is critical to parasite elimination and host tissue damage caused by an immune response. Herein, we go into the cellular and molecular mechanisms and targets of the MedDiet fatty acids in the host and the parasite, reviewing potential benefits of the MedDiet, on inflammation, malaria infection progression, and clinical outcome.

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Autophagic induction modulates splenic plasmacytoid dendritic cell mediated immune response in cerebral malarial infection model

Cited by 15 publications

References 47 publications

Anionic and Cationic Lipid Adjuvants individually induce distinct adaptive Th1/Th2-type immunity in enhancing post-intranasal immunization response against influenza

Anionic and Cationic Lipid Adjuvants individually induce distinct adaptive Th1/Th2-type immunity in enhancing post-intranasal immunization response against influenza

Targeting Autophagy in Innate Immune Cells: Angel or Demon During Infection and Vaccination?

Mediterranean Diet: Lipids, Inflammation, and Malaria Infection

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