Autonomous Parvovirus and Densovirus Gene Vectors

Corsini, Joe; Afanasiev, Boris N.; Maxwell, Ian H.; Carlson, Jonathan O.

doi:10.1016/s0065-3527(08)60738-1

Cited by 30 publications

(16 citation statements)

References 209 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cloning of the entire JcDNV genome into pBR322 led to a recombinant construct (pBRJ) that retained the capacity to produce infectious particles when injected into larvae of the sensitive host Spodoptera littoralis or transfected into lepidopteran cell lines (20,24,25). The availability of plasmids carrying an infectious sequence of either the JcDNV or the Aedes aegypti densovirus genome has prompted the study of densoviruses as expression vectors (1,2,5,10,16,31). The A. aegypti densovirus, a prototype of the Brevidensovirus genus, has been developed as a gene transfer vehicle that is able to transduce genes into mosquito larvae by typical routes of in-fection, opening the potential for gene introduction into natural populations (1,2).…”

Section: The Invertebrate Parvovirusmentioning

confidence: 99%

Junonia coenia Densovirus-Based Vectors for Stable Transgene Expression in Sf9 Cells: Influence of the Densovirus Sequences on Genomic Integration

Bossin

Fournier

Royer

et al. 2003

J Virol

View full text Add to dashboard Cite

show abstract

Section: The Invertebrate Parvovirusmentioning

confidence: 99%

Junonia coenia Densovirus-Based Vectors for Stable Transgene Expression in Sf9 Cells: Influence of the Densovirus Sequences on Genomic Integration

Bossin

Fournier

Royer

et al. 2003

J Virol

View full text Add to dashboard Cite

show abstract

“…3 They can also be used as vectors for gene delivery. 4,22,30 These viruses therefore have potential as therapeutic agents against cancer, either by exploiting their natural oncolytic properties or by using them for delivery of therapeutic genes. Prospects for in vivo use would be improved by viral modification conferring specific targeting of infection to human tumor cells.…”

Section: Discussionmentioning

confidence: 99%

“…Autonomous parvoviruses are small, icosahedral, single strand DNA viruses that replicate preferentially in tumor cells [1][2][3][4] and, unlike the adeno-associated parvovirus, AAV, do not require a helper virus for replication. Several members of the rodent group of autonomous parvoviruses replicate efficiently in human transformed cell lines.…”

Section: Introductionmentioning

confidence: 99%

Expansion of tropism of a feline parvovirus to target a human tumor cell line by display of an αv integrin binding peptide on the capsid

et al. 2001

View full text Add to dashboard Cite

The autonomous parvoviruses are small, non-enveloped, single strand DNA viruses. They occur in many species and they have oncolytic properties. We are modifying the capsid of feline panleukopenia virus (FPV), a parvovirus which normally infects feline cells, with the goal of targeting human tumor cells for potential cancer therapy. Using recombinant viruses transducing a luciferase reporter, we show that insertion of a cyclically constrained, integrin-binding peptide at an exposed position on the FPV capsid enables transduction of an ␣v integrin-expressing human rhabdomyosarcoma cell line (Rh18A). These cells were not transduced by virus

show abstract

“…The production of recombinant MVM(p) and autonomous parvovirus vectors in general is hampered by the low efficiency of recombinant virus production after transfection of permissive cells and by the generation of WT virus that prevents amplification in permissive cells (11). The WT virus is generated through homologous recombination between vector and helper DNAs.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Sequencing of Defective Particles Derived from the Autonomous Parvovirus Minute Virus of Mice for the Construction of Vectors with Minimal cis -Acting Sequences

Clément

Avalosse

Bakkouri

et al. 2001

J Virol

View full text Add to dashboard Cite

The production of wild-type-free stocks of recombinant parvovirus minute virus of mice [MVM(p)] is difficult due to the presence of homologous sequences in vector and helper genomes that cannot easily be eliminated from the overlapping coding sequences. We have therefore cloned and sequenced spontaneously occurring defective particles of MVM(p) with very small genomes to identify the minimal cis-acting sequences required for DNA amplification and virus production. One of them has lost all capsid-coding sequences but is still able to replicate in permissive cells when nonstructural proteins are provided in trans by a helper plasmid. Vectors derived from this particle produce stocks with no detectable wild-type MVM after cotransfection with new, matched, helper plasmids that present no homology downstream from the transgene.The autonomous parvovirus minute virus of mice [MVM(p)] is a lytic virus that replicates as an episome, preferentially in transformed cells. Given its oncotropism but also its innocuousness in the adult host and its resistance to extreme pH and temperatures, MVM(p) has been proposed as a vector for the gene therapy of cancer (34). The 5-kb single-stranded DNA genome of MVM(p) is organized into two overlapping transcription units. The early P4 promoter controls transcription of the nonstructural proteins, NS1 and NS2. The pleiotropic NS1 protein is responsible for the cytotoxic activity of MVM(p) in transformed cells (6, 10), is required for viral DNA amplification, and can positively or negatively affect the activity of homologous or heterologous promoters (26). NS1 also transactivates the second promoter, P38, thus allowing the synthesis of capsid proteins VP1 and VP2 at the end of the viral cycle (15).We have previously derived a recombinant parvovirus from MVM(p) that transfers the cDNA for human interleukin-2 (IL-2). This virus is defective since part of the genes coding for capsid proteins (VP) is deleted. Transducing recombinant virus can, however, be produced if VP proteins are provided in trans by a cotransfected helper plasmid (35) or from genes integrated into the host chromosome (packaging cells) (7, 17). However, these stocks are contaminated by wild-type (WT) virus, which renders impossible their amplification through serial infections. The WT virus is generated through homologous recombination between vector and helper DNAs. Ideally, removing all sequence homology between these DNA sequences should prevent the formation of WT virus. However, data from Astell et al. suggest that a cis-acting element, necessary for DNA replication, overlaps the capsid-coding genes (2). On the other hand, data obtained from the study of defective particles indicate that particles as small as 15% of the size of WT MVM(p) are able to replicate. These defective particles are generated spontaneously during high-multiplicity infections (19) and are selectively amplified during serial infections. They vary in size from 15 to 70% of that of WT MVM(p), but they always retain the two terminal palindromes of 1...

show abstract

Autonomous Parvovirus and Densovirus Gene Vectors

Cited by 30 publications

References 209 publications

Junonia coenia Densovirus-Based Vectors for Stable Transgene Expression in Sf9 Cells: Influence of the Densovirus Sequences on Genomic Integration

Junonia coenia Densovirus-Based Vectors for Stable Transgene Expression in Sf9 Cells: Influence of the Densovirus Sequences on Genomic Integration

Expansion of tropism of a feline parvovirus to target a human tumor cell line by display of an αv integrin binding peptide on the capsid

Cloning and Sequencing of Defective Particles Derived from the Autonomous Parvovirus Minute Virus of Mice for the Construction of Vectors with Minimal cis -Acting Sequences

Contact Info

Product

Resources

About