Junonia coenia Densovirus-Based Vectors for Stable Transgene Expression in Sf9 Cells: Influence of the Densovirus Sequences on Genomic Integration

Abstract

The invertebrate parvovirus

The Encapsidated Genome of Microplitis demolitor Bracovirus Integrates into the Host Pseudoplusia includens

“…Currently, the only known animal DNA virus to exhibit site-directed integration is adenoassociated virus (AAV), where nonhomologous recombination into different locations of the AAVS1 domain in human chromosome 19 involves interactions between virus-encoded proteins, palindrome-forming terminal inverted repeats in the viral genome, and host cell machinery (9,18,19,24,44). The integration of another group of parvoviruses (densoviruses) that infect insects may also involve unknown host cell factors interacting with viral sequences (8).…”

Identification of Structural Proteins of <i>Bombyx mori</i> Parvo-Like Virus (China Zhenjiang Isolate)

“…An examination of the polypeptide obtained by Hayakawa et al [14] from the expression of the N-terminal 1,000 nucleotides of BmDNV-2 VD1-ORF1 indicated that the polypeptide's C-terminus is the basic region with an isoelectric point of around 8. 16. We speculated that this basic region had a similar function as histone in eukaryotic chromosomal organization and therefore was the viral nucleoprotein.…”

“…Indeed, the observation that several structural proteins translate from one ORF is very common in parvoviruses. For instance, VPs of densonucleosis viruses of Galleria mellonella , Junonia coenia and Mythimna loreyi are all translated from one ORF by a leaky scanning mechanism [15][16][17] .…”

A genomic BAC library and a new BAC-GFP vector to study the holocentric pest Spodoptera frugiperda

“…We also describe a new vector for use in transient transfection of cell lines of Spodoptera and related species. This vector is a pBAC3.6e vector derivative (Frengen et al, 1999) carrying a GFP marker under the control of an insect promoter, the densoviral P9 promoter (Bossin et al, 2003), which is functional in numerous Lepidoptera including B. mori. Since it is a BAC vector, it can accommodate large genomic inserts.…”