Autonomous Growth of Isolated Single
            <i>Listeria monocytogenes</i>
            and
            <i>Salmonella enterica</i>
            Serovar Typhimurium Cells in the Absence of Growth Factors and Intercellular Contact

Roeder, Barbara; Wagner, Martin; Rossmanith, Peter

doi:10.1128/aem.01506-09

Cited by 4 publications

(15 citation statements)

References 37 publications

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“…Micromanipulation has been applied to single bacterial cells since 1960s (Nossal et al, 1964;Wood, 1967). In mechanical micromanipulation, single cells are individually captured from a population and transferred using a micropipette (Anis et al, 2010(Anis et al, , 2011Ashida et al, 2010;Gao et al, 2011;Roeder et al, 2010;Shi et al, 2011;Teramoto et al, 2010;Tsang et al, 2006). The isolated single cells can be subsequently used for different applications such as cultivation or gene expression analysis.…”

Section: Micromanipulationmentioning

confidence: 99%

Measuring gene expression in single bacterial cells: recent advances in methods and micro-devices

Shi

Gao

Wang

et al. 2014

Critical Reviews in Biotechnology

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Populations of bacterial cells that grow under the same conditions and/or environments are often considered to be uniform and thus can be described by ensemble average values of their physiologic, phenotypic, genotypic or other parameters. However, recent evidence suggests that cell-to-cell differences at the gene expression level could be an order of magnitude greater than previously thought even for isogenic bacterial populations. Such gene expression or transcriptional-level heterogeneity determines not only the fate of individual bacterial cells in a population but could also affect the ultimate fate of the population itself. Although techniques for single-cell gene expression measurement in eukaryotic cells have been successfully implemented for a decade or so, they have only recently become available for single bacterial cells. This is due to the difficulty of efficient lysis of most bacterial cells, as well as short half-life time (low stability) of bacterial mRNA. In this article, we review the recent progress and challenges associated with analyzing gene expression levels in single bacterial cells using various semi-quantitative and quantitative methods. In addition, a review of the recent progress in applying microfluidic devices to isolate single bacterial cells for gene expression analysis is also included.

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Section: Micromanipulationmentioning

confidence: 99%

Measuring gene expression in single bacterial cells: recent advances in methods and micro-devices

Shi

Gao

Wang

et al. 2014

Critical Reviews in Biotechnology

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“…Nevertheless, results from the study by Schoder et al. [IAFP, 97th Annual Meeting, JFPRDR, 73(Sup), p. 93] indicate that this is sufficiently performed only in unselective media as also demonstrated by Roeder et al. (2010a).…”

Section: Introductionmentioning

confidence: 98%

“…Based on data derived from the investigation into dormant cells, growth factors have been investigated for several species (Kaprelyants and Kell 1996; Roeder et al. 2010a).…”

Section: Introductionmentioning

confidence: 99%

“…Roeder et al. (2010a) developed a method capable of serial manipulation of single prokaryotic cells under visual control.…”

Section: Introductionmentioning

confidence: 99%

“…The dilution factor obtained during the cell washing procedure was 1 × 10 −10 in this study. The results of the study carried out by Roeder et al. (2010a) suggest that single prokaryotic cells are capable of proliferation independently from growth factors and cellular contact.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The challenge to quantify Listeria monocytogenes- a model leading to new aspects in molecular biological food pathogen detection

Rossmanith

Wagner

2010

Journal of Applied Microbiology

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Summary In this work, we discuss the latest insights concerning advantages and disadvantages and the nature of microbiological and molecular methods for quantitative food pathogen detection. The assessment of molecular methods must be brought on a basis that considers the nature of molecular methods and their underlying mechanism. A potential approach to setting up the development, validation and structure of an analytical chain is presented based on quantitative real‐time PCR (qPCR). This is analysed exemplary on the basis of recent work using the model organism Listeria monocytogenes. Several prerequisites for successful quantitative detection of this pathogen will be discussed. In particular, sample preparation, controls for all methodical steps and the validation of the core assay qPCR are addressed, which constitute the basis for a reliable analytical detection chain for molecular biological pathogen detection from food. Microbiological methods are analysed based on growth of the single cell, which is the fundament of these traditional methods.

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Evolution of pH-sensitive transcription termination in Escherichia coli during adaptation to repeated long-term starvation

Worthan,

McCarthy,

Delaleau

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Fluctuating environments that consist of regular cycles of co-occurring stress are a common challenge faced by cellular populations. For a population to thrive in constantly changing conditions, an ability to coordinate a rapid cellular response is essential. Here, we identify a mutation conferring an arginine-to-histidine (Arg to His) substitution in the transcription terminator Rho. The rho R109H mutation frequently arose in Escherichia coli populations experimentally evolved under repeated long-term starvation conditions, during which the accumulation of metabolic waste followed by transfer into fresh media results in drastic environmental pH fluctuations associated with feast and famine. Metagenomic sequencing revealed that populations containing the rho mutation also possess putative loss-of-function mutations in ydcI , which encodes a recently characterized transcription factor associated with pH homeostasis. Genetic reconstructions of these mutations show that the rho allele confers plasticity via an alkaline-induced reduction of Rho function that, when found in tandem with a Δ ydcI allele, leads to intracellular alkalization and genetic assimilation of Rho mutant function. We further identify Arg to His substitutions at analogous sites in rho alleles from species that regularly experience neutral to alkaline pH fluctuations in their environments. Our results suggest that Arg to His substitutions in Rho may serve to rapidly coordinate complex physiological responses through pH sensing and shed light on how cellular populations use environmental cues to coordinate rapid responses to complex, fluctuating environments.

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Autonomous Growth of Isolated Single Listeria monocytogenes and Salmonella enterica Serovar Typhimurium Cells in the Absence of Growth Factors and Intercellular Contact

Cited by 4 publications

References 37 publications

Measuring gene expression in single bacterial cells: recent advances in methods and micro-devices

Measuring gene expression in single bacterial cells: recent advances in methods and micro-devices

The challenge to quantify Listeria monocytogenes- a model leading to new aspects in molecular biological food pathogen detection

Evolution of pH-sensitive transcription termination in Escherichia coli during adaptation to repeated long-term starvation

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