Autonomic innervation and plasma estradiol-17? and progesterone levels in rats with subcutaneous ovarian autografts

Chihal, H. J.; Weitsen, Howard A.; Stone, Scott; Peppler, R. D.

doi:10.1007/bf00220827

Cited by 6 publications

(5 citation statements)

References 10 publications

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“…A definite correlation was detected between the vaginal smear and plasma levels of steroid hormones in the castrated female rat with subcutaneous ovarian autographs. Chihal et al [27] identify that castrates which received ovarian autografts developed cycling vaginal smears and had increased estradiol (31.9 + / − 4.3 pg/ml) and decreased progesterone (18.3 + / − 1.9 ng/ml) levels. Also in the present study, there were seen cyclic variations in the vaginal irrigation samples at the group 1 and group 2.…”

Section: Discussionmentioning

confidence: 99%

A study of ovarian autotransplantation without vascular a pedicle in rats

Rişvanlı

Timurkan

Akpolat

et al. 2006

J Assist Reprod Genet

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Purpose: The study aimed to determine effects of FSH applications on follicle survival, development and hormone output and antigenicity of rat ovarian tissue autografts placed at subcutaneous or subperitoneal sites.Methods: A total of sixteen female rats were used in the study. The animals were divided into three groups. Ovaries were dissected and then transplanted under the peritoneum in the first group animals (n = 5) or under the skin in the second group animals (n = 6). And the animals in the third group (n = 5) were sham operated. Following operations, intramuscular injection of 8 IU of rhFSH were made daily to the animals in first and second groups from the first day of operation through thirty days. Vaginal irrigation samples were prepared daily from the animals for 30 days. The concentrations of serum estradiol and antiovarian antibodies in the blood were determined using ELISA on the last day Results: Results showed that cyclic variations were noticed in the samples of vaginal irrigation by day 30 in the animals of first and second groups. However, no significant differences were seen between groups. The concentration of blood serum estradiol was higher in the animals of first group. Decrease in numbers of primary follicles were found in the animals of second group and lesser corpus luteum were found in the animals of control group on the histopathological examinations of transplanted ovaries. All rats in the first and second groups were defined as seropositive for antiovarian antibodies. When the OD values were compared between first and second groups, it was identified that the OD values of rats in the first group was higher than it was seen in the second group.Conclusion: The ovarian transplantation without vascular pedicle in rats is characterized by follicular hyperplasia endocrinologically functional. Being seropositive of all rats in first and second groups in terms of antiovarian antibodies is an indicator to these antibodies does not affect the functions of transplanted ovaries. It is believed that the highness of OD values in the group which is transplanted beneath the peritoneum is based on the highness of estradiol concentrations in these animals.

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Section: Discussionmentioning

confidence: 99%

A study of ovarian autotransplantation without vascular a pedicle in rats

Rişvanlı

Timurkan

Akpolat

et al. 2006

J Assist Reprod Genet

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“…Ovaries autotransplanted to the neck of sheep are reported to contain higher than normal amounts of cholinesterase (Harrison, Heap & Silver, 1974). Chihal et al (1976) observed that subcutaneous ovarian autografts in rats exhibited more acetylcholinesterase activity while Jordan (1971) found control ovaries normally contained no cholinergic fibres. Bulmer (1965) observed high butyrylcholinesterase activity in the ovaries of several species, but only in the monkey was this apparently associated with nerve fibres.…”

Section: Ovarian Innervationmentioning

confidence: 99%

“…However, a difficulty precluding acceptance of this view is that the time taken for reinnervation and revasculation of ovarian grafts is similar to that taken for reestablishment of oestrous behaviour. Thus in a sheep reestablishment of oestrous behaviour after ovarian transplant during a pregnancy took approximately a year (Harrison et al, 1974), in the rat, 9 days (Harris & Eakin, 1949;Chihal et al, 1976) and in the Macaque monkey 6-8 weeks (Jaszczask & Hafez, 1963). In the rabbit following autografting of ovaries to the ovarian bursa or to the lateral abdominal wall a 10-17 day delay occurred before the reappearance of hormonal function.…”

Section: Innervation Of the Ovarian Ligamentmentioning

confidence: 99%

“…The atypically high levels of acetylcholinesterase associated with ovarian autografts (Harrison et al, 1974;Chihal, et al, 1976) add a further complication. In the experiments of Chihal et al (1976), using rats, the first metoestrous after grafting occurred 9 days after surgery, at which time autonomic innervation remained very variable and large nerve fascicles not seen in control ovaries were present. In these experiments no positive evidence of ovulation such as extruded ova were found; indeed many grafts displayed luteal tissue in which ova were entrapped.…”

Section: Effects Of Ovarian Denervation Transplan Ration and Drugs Mmentioning

confidence: 99%

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The Sympathetic Innervation of the Mammalian Ovary. A Review of Pharmacological and Histological Studies

Mohsin

Pennefather

1979

Clin Exp Pharmacol Physiol

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The a i m of this article is to review the literature concerning the nature and function of the sympathetic innervation of the mammalian ovary. We focus our attention upon evidence relating to suggestions that ovarian smooth muscle participates in ovulatory events, and that such participation may be modulated by the local neural supply. Guttmacher & Guttmacher (1921) mentioned that this possibility was first suggested nearly 70 years before they observed smooth muscle-like cells in the follicular walls of sow ovaries and found that isolated follicular walls were contracted by physostigmine and relaxed by adrenaline. However, Claessen (1 947), using polarisation microscopy, was unable to confirm the presence of smooth muscle in the apices of follicles from a number of species, and interest in the Guttmachers' findings temporarily lapsed.In 1967, Jacobowitz & Wallach reported the findings of a histological study of ovaries from cats, monkeys and humans in which they observed catecholamine fluorescence associated with smooth muscle-like cells in the stroma. In 1969, Rocereto, Jacobowitz & Wallach demonstrated that the cat isolated ovary exhibited spontaneous mechanical activity and was reactive to catecholamines. These investigations together with those of Owman & Sjoberg (1966) and Sjoberg (1967) initiated a resurgence of interest in the possibility that the adrenergic innervation of the ovary may play a functional role in ovarian processes; in particular that it might affect the contractility of smooth muscle and so participate in ovulation.With the development of techniques such as electron microscopy and fluorescence histochemistry, together with greater sophistication of pharmacodynamic analysis, an increasing number of publications concerned with the role of smooth muscle and adrenergic innervation in the ovary has appeared in the recent literature.Reference to some of this work is found in the reviews of Marshall (1972, 1973) t and Bell (1972). A comprehensive review devoted specifically to the roles of catecholamines and nerves in ovulation was published by Bahr, Kao & Nalbandov in 1974. Their review described the sympathetic innervation of avian as well as of mammalian ovaries and the possible function of such innervation. They considered evidence relating to several possible roles, including modification of contraction of ovarian smooth muscle, participation in ovulation, and/or control of the ovarian vasculature, and concluded that nerves play some role in ovulation, but that it is not clear how this effect is mediated.

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“…Capps, Lawrence & Burden (1978) showed that stimulation of the ovarian plexus nerve in hypophysectomized rats induced morphological changes in the interstitial gland characteristic of active steroidogenic cells. Moreover, plasma progesterone concentrations were decreased on the first day of metoestrus that occurred in ovariectomized female rats bearing subcutaneous ovarian grafts (Chihal, Weitsen, Stone & Peppier, 1976). These responses of interstitial gland cells to denervation and stimulation provided morphological evidence for a functional role for the adrenergic nerves to the ovarian compartment.…”

Section: Introductionmentioning

confidence: 97%

Evidence for direct neural control of ovarian steroidogenesis in rats

Weiss¹,

Dail²,

Ratner³

1982

Reproduction

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Electrical stimulation of the superior ovarian nerve of intact anaesthetized dioestrous rats for 30 min reduced ovarian progesterone levels, even when papaverine and propranolol were also given. The administration of phentolamine (an alpha receptor antagonist) before stimulation reversed this effect. The results suggest that a neural control of ovarian steroidogenesis may be either excitatory through the stimulation of beta receptors or inhibitory through the stimulation of alpha receptors.

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Autonomic innervation and plasma estradiol-17? and progesterone levels in rats with subcutaneous ovarian autografts

Cited by 6 publications

References 10 publications

A study of ovarian autotransplantation without vascular a pedicle in rats

A study of ovarian autotransplantation without vascular a pedicle in rats

The Sympathetic Innervation of the Mammalian Ovary. A Review of Pharmacological and Histological Studies

Evidence for direct neural control of ovarian steroidogenesis in rats

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