Automation of the protein crystallography beamline X06DA at the Swiss Light Source

Wang, M.; Schneider, Roman; Pradervand, Claude; Glettig, Wayne; Roccamante, Mauro; Tanaka, Takashi; Panepucci, Ezequiel; Isenegger, A.; Zimoch, Elke; Kalt, H.; Baechli, H.; Chen, Q.; Schulze-Briese, Clemens

doi:10.1107/s0108767308094373

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This resulted in a mini -goniometer head version 3 (MK3) that is fully compatible with the MD2 and MD2M suite of diffractometers installed on many MX beamlines (Cipriani et al, 2007;Brockhauser et al, 2007). Indeed, similar miniaturized -goniometer instruments have also been developed elsewhere (Wang et al, 2008;Shi et al, 2006;Rosenbaum & Westbrook, 1997;Rosenbaum et al, 2006). Four MK3s are now permanently installed and available on the EMBL-ESRF Joint Structural Biology Group beamlines at the ESRF.…”

Section: Introductionmentioning

confidence: 99%

The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments

Brockhauser

Ravelli

McCarthy

2013

Acta Crystallogr D Biol Cryst

View full text Add to dashboard Cite

Most macromolecular crystallography (MX) diffraction experiments at synchrotrons use a single-axis goniometer. This markedly contrasts with small-molecule crystallography, in which the majority of the diffraction data are collected using multi-axis goniometers. A novel miniaturized -goniometer head, the MK3, has been developed to allow macromolecular crystals to be aligned. It is available on the majority of the structural biology beamlines at the ESRF, as well as elsewhere. In addition, the Strategy for the Alignment of Crystals (STAC) software package has been developed to facilitate the use of the MK3 and other similar devices. Use of the MK3 and STAC is streamlined by their incorporation into online analysis tools such as EDNA. The current use of STAC and MK3 on the MX beamlines at the ESRF is discussed. It is shown that the alignment of macromolecular crystals can result in improved diffraction data quality compared with data obtained from randomly aligned crystals.

show abstract

Section: Introductionmentioning

confidence: 99%

The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments

Brockhauser

Ravelli

McCarthy

2013

Acta Crystallogr D Biol Cryst

View full text Add to dashboard Cite

show abstract

“…Other beamlines and tube-based X-ray scanners have implemented automated systems for sample management and exchange; specifically, the tomography beamline of 2-BM at the Advanced Photon Source (De Carlo et al, 2006;Wang et al, 2001;De Carlo, 2010), several protein crystallography and diffraction beamlines (Olieric et al, 2009;Wang et al, 2008;De Carlo & Tieman, 2004;Yamamoto et al, 2005;Alzari et al, 2006;Moser et al, 2005), and some SCANCO mCT scanners (SCANCO Medical AG, 2009).…”

Section: Introductionmentioning

confidence: 99%

High-throughput full-automatic synchrotron-based tomographic microscopy

Mäder

Marone

Hintermüller

et al. 2011

J Synchrotron Radiat

View full text Add to dashboard Cite

At the TOMCAT (TOmographic Microscopy and Coherent rAdiology experimenTs) beamline of the Swiss Light Source with an energy range of 8-45 keV and voxel size from 0.37 mm to 7.4 mm, full tomographic datasets are typically acquired in 5 to 10 min. To exploit the speed of the system and enable high-throughput studies to be performed in a fully automatic manner, a package of automation tools has been developed. The samples are automatically exchanged, aligned, moved to the correct region of interest, and scanned. This task is accomplished through the coordination of Python scripts, a robot-based sample-exchange system, sample positioning motors and a CCD camera. The tools are suited for any samples that can be mounted on a standard SEM stub, and require no specific environmental conditions. Up to 60 samples can be analyzed at a time without user intervention. The throughput of the system is dependent on resolution, energy and sample size, but rates of four samples per hour have been achieved with 0.74 mm voxel size at 17.5 keV. The maximum intervention-free scanning time is theoretically unlimited, and in practice experiments have been running unattended as long as 53 h (the average beam time allocation at TOMCAT is 48 h per user). The system is the first fully automated high-throughput tomography station: mounting samples, finding regions of interest, scanning and reconstructing can be performed without user intervention. The system also includes many features which accelerate and simplify the process of tomographic microscopy.

show abstract

Automation of the protein crystallography beamline X06DA at the Swiss Light Source

Cited by 2 publications

References 0 publications

The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments

The use of a mini-κ goniometer head in macromolecular crystallography diffraction experiments

High-throughput full-automatic synchrotron-based tomographic microscopy

Contact Info

Product

Resources

About