Automation of Immunoassays

Chan, Daniel W.

doi:10.1016/b978-012214730-2/50022-4

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…Bovine intestine ALP (BIALP) has the highest specific activity among mammalian ALPs. Therefore, it has been applied as a signaling enzyme in sensitive assays such as enzyme immunoassay (EIA), Western blotting analysis, nucleic acid hybridization assay, and polymerase chain reaction and has been used in diagnosis, immunology, and molecular biology [9][10][11]. We developed a fully automated random-accessible type EIA diagnosis system, AIA, with BIALP (Tosoh, Tokyo, Japan).…”

Section: Introductionmentioning

confidence: 99%

Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity

Sekiguchi

Hashida

Yasukawa

et al. 2011

Enzyme and Microbial Technology

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Bovine intestine alkaline phosphatase (BIALP) is widely used as a signaling enzyme in sensitive assays such as enzyme immunoassay (EIA). In this study, we evaluated the effects of various aminoalcohols and amines on the activity of BIALP in the hydrolysis of p-nitrophenyl phosphate (pNPP) at pH 9.8, at 20 °C. The k(cat) values at 0.05 M diethanolamine, 0.1 M triethanolamine, and 0.2 M N-methylethanolamine were 190±10, 840±30, and 500±10 s(-1), respectively. The k(cat) values increased with increasing concentrations of diethanolamine, triethanolamine, and N-methylethanolamine and reached 1240±60, 1450±30, and 2250±80 s(-1), respectively, at 1.0M. On the other hand, the k(cat) values at 0.05-1.0M ethanolamine, ethylamine, methylamine, and dimethylamine were in the range of 100-600 s(-1). These results indicate that diethanolamine, triethanolamine and N-methylethanolamine highly activate BIALP and might be suitable as a dilution buffer of BIALP in EIA. Interestingly, the K(m) values increased with increasing concentrations of diethanolamine and N-methylethanolamine, but not triethanolamine: the K(m) value at 1.0M diethanolamine (0.83±0.15 mM) was 12-fold higher than that at 0.05M (0.07±0.01 mM), and that at 1.0M N-methylethanolamine (2.53±0.20 mM) was 14-fold higher than that at 0.2M (0.18±0.02 mM), while that at 1.0M triethanolamine (0.31±0.01 mM) was similar as that at 0.2M (0.25±0.01 mM), suggesting that the mechanisms of BIALP activation are different between the aminoalcohols.

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Section: Introductionmentioning

confidence: 99%

Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity

Sekiguchi

Hashida

Yasukawa

et al. 2011

Enzyme and Microbial Technology

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“…2) as well as less restriction on utilisation-the main demonstration of this being the development of automated immunoassay analysers. 15,16 In this case the use of an enzyme was not designed to take advantage of its specificity but rather of its (hopefully) high turnover number leading to the possibility of signal amplification.…”

Section: Antibody Binding Reactionsmentioning

confidence: 99%

Application of chemistry to in vitro diagnostic tests

Price¹

2001

Chem. Soc. Rev.

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“…This broad subject spans IA technology, automation technology, and method evaluation, so coverage will be selective, emphasizing the interfaces among these areas. In view of the rapid evolution of commercial systems, and detailed descriptions available elsewhere, [1][2][3][4][5] discussion will be generic, with mention of specific systems only as examples.…”

Section: Use Of Immunoassays and Other Ligand-binding Assays In Clinimentioning

confidence: 99%

“…For IA, this can be accomplished not only with mechanical instruments, but also with self-enclosed single-test systems intended for point-of-care or home use. 1…”

Section: Definition and Scopementioning

confidence: 99%

The New Era of Automated Immunoassay

Bock

2000

American Journal of Clinical Pathology

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Use of immunoassays and other ligand-binding assays in clinical diagnosis has increased dramatically during the last several years. Despite impressive technical advances, "mass production" of these assays in a routine laboratory still presents many difficulties. This review of ligand-binding assay technology highlights some recent developments, emphasizing challenges and possible solutions for cost-effective patient care.

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Automation of Immunoassays

Cited by 7 publications

References 16 publications

Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity

Effects of amines and aminoalcohols on bovine intestine alkaline phosphatase activity

Application of chemistry to in vitro diagnostic tests

The New Era of Automated Immunoassay

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