Automatic sample preparation in commercial capillary-electrophoresis equipment

Santos, Bricio; Valcárcel, Miguel; Simonet, B.M.; Ríos, Ángel

doi:10.1016/j.trac.2006.07.008

Cited by 33 publications

(23 citation statements)

References 30 publications

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“…The same type of sorbent, monolithic column with Cu(II) iminodiacetic acid functional groups has been developed for preconcentration of low-abundance peptides and proteins from complex biological samples [53]. The practical considerations that permit coupling or integration of sample treatment devices into commercial CE apparatuses are treated in the recent review [54] where the off-line and on-line strategies are critically compared and the integrated in-capillary, in-vial, or in-replenishment-system methodologies for SPME and LLE are described.…”

Section: Preconcentration and Preseparationmentioning

confidence: 99%

Recent developments in CE and CEC of peptides

Kašička

2007

Electrophoresis

118

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The article brings a comprehensive survey of recent developments and applications of high-performance capillary electromigration methods, zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. New approaches to the theoretical description and experimental verification of electromigration behavior of peptides and to methodology of their separations, such as sample preparation, adsorption suppression, and detection, are presented. Novel developments in individual CE and CEC modes are shown and several types of their applications to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Preconcentration and Preseparationmentioning

confidence: 99%

Recent developments in CE and CEC of peptides

Kašička

2007

Electrophoresis

118

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“…Inserting the split-flow interface into the CE equipment required introducing some reversible modifications in order to prevent the vial elevator from colliding with the interface. A detailed description of such modifications is provided elsewhere [14,21]. A T-piece was used to ground the flow system with an electrical wire.…”

Section: Manifoldmentioning

confidence: 99%

“…The latter two are especially interesting for routine laboratories as they simplify the analyst's work and reduce errors due to human intervention. Interested readers are referred to a general overview for a detailed description of their implementation in routine laboratories [14].…”

Section: Introductionmentioning

confidence: 99%

Electrical field‐assisted solid‐phase extraction coupled on‐line to capillary electrophoresis‐mass spectrometry

et al. 2008

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A substantial demand currently exists for analytical methods affording the determination of very low concentrations of analytes in complex matrices, such as those of environmental and biological samples, as simply as possible. However, the pretreatment of complex samples, which is unavoidable prior to CE-MS analysis, is usually complicated and time-consuming. In this work, we used voltage-assisted SPE for the first time as an alternative to conventional treatments for preconcentrating and purifying analytes. To this end, we used a simple flow system coupled on-line to CE-MS equipment. The system is quite robust and provides reproducible peak areas (the precision ranges from 2.5 to 3.8%). Also, it provides increased sensitivity affording the determination of trace amounts (nanogram per liter levels) of analytes in only a few milliliters of sample. The proposed system was applied to the determination of members of two compound families (viz. tetracyclines and amines).

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“…In 2006, some members of our research group presented an interesting paper to support the transfer of advances from CE research laboratories to routine laboratories. They focused their research on the integration of sample treatment devices into commercial CE equipment [13]. In 2005, Castañeda et al [14] reviewed some analytical approaches to demonstrate the analytical usefulness of CE in routine food analysis.…”

Section: Introductionmentioning

confidence: 99%

Thirty years of capillary electrophoresis in food analysis laboratories: Potential applications

2011

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CE has generated considerable interest in the research community since instruments were introduced by different trading companies in the 1990s. Nowadays, CE is popular due to its simplicity, speed, highly efficient separations and minimal solvent and reagent consumption; it can also be included as a useful technique in the nanotechnology field and it covers a wide range of specific applications in different fields (chemical, pharmaceutical, genetic, clinical, food and environmental). CE has been very well evaluated in research laboratories for several years, and different new approaches to improve sensitivity (one of the main drawbacks of CE) and robustness have been proposed. However, this technique is still not well accepted in routine laboratories for food analysis. Researching in data bases, it is easy to find several electrophoretic methods to determine different groups of analytes and sometimes they are compared in terms of sensitivity, selectivity, precision and applicability with other separation techniques. Although these papers frequently prove the potential of this methodology in spiked samples, it is not common to find a discussion of the well-known complexity of the matrices to extract analytes from the sample and/or to study the interferences in the target analytes. Summarizing, the majority of CE scientific papers focus primarily on the effects upon the separation of the analytes while ignoring their behavior if these analytes are presented in real samples.

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Automatic sample preparation in commercial capillary-electrophoresis equipment

Cited by 33 publications

References 30 publications

Recent developments in CE and CEC of peptides

Recent developments in CE and CEC of peptides

Electrical field‐assisted solid‐phase extraction coupled on‐line to capillary electrophoresis‐mass spectrometry

Thirty years of capillary electrophoresis in food analysis laboratories: Potential applications

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