Automated vitrification of mammalian embryos on a digital microfluidic device

Pyne, Derek; Liu, Jun; Abdelgawad, Mohamed; Sun, Yu

doi:10.1109/memsys.2014.6765769

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…However, microfluidics is, at its very core, developed for handling very small volumes. Recent reviews and studies have been published that can help the ART community to address problems in effective cryopreservation of sperm from microTESE samples by utilizing microfluidic technology [Zhang et al 2012;Zhang et al 2011b;Song et al 2009;Li et al 2010;Pyne and Liu 2014]. Some of these works that seem promising for single or low number sperm cryopreservation are discussed below.…”

Section: Microfluidics For Sperm Cryopreservationmentioning

confidence: 99%

Microfluidics: The future of microdissection TESE?

Samuel

Badamjav

Murphy

et al. 2016

Systems Biology in Reproductive Medicine

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Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic technology and single-cell Raman spectroscopy.

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Section: Microfluidics For Sperm Cryopreservationmentioning

confidence: 99%

Microfluidics: The future of microdissection TESE?

Samuel

Badamjav

Murphy

et al. 2016

Systems Biology in Reproductive Medicine

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“…Derek et al have reported the development of a DMF for the vitrification of mammalian embryos for applications in clinical IVF. 34 Now, our research team is also using the DMF platform in IVF researches, and this is the reason why the mouse embryos were used in this experiment for droplets manipulation. This high-quality dielectric DMF chip has so far proved its potential for chemical and biomedical sample processing; we expect to use this device in future research on IVF.…”

Section: Proposed Ewod System For Biomedical Applicationsmentioning

confidence: 99%

Improving the dielectric properties of an electrowetting-on-dielectric microfluidic device with a low-pressure chemical vapor deposited Si3N4 dielectric layer

2015

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Dielectric breakdown is a common problem in a digital microfluidic system, which limits its application in chemical or biomedical applications. We propose a new fabrication of an electrowetting-on-dielectric (EWOD) device using Si 3 N 4 deposited by low-pressure chemical vapor deposition (LPCVD) as a dielectric layer. This material exhibits a greater relative permittivity, purity, uniformity, and biocompatibility than polymeric films. These properties also increase the breakdown voltage of a dielectric layer and increase the stability of an EWOD system when applied in biomedical research. Medium droplets with mouse embryos were manipulated in this manner. The electrical properties of the Si 3 N 4 dielectric layer-breakdown voltage, refractive index, relative permittivity, and variation of contact angle with input voltage-were investigated and compared with a traditional Si 3 N 4 dielectric layer deposited as a plasma-enhanced chemical vapor deposition to confirm the potential of LPCVD Si 3 N 4 applied as the dielectric layer of an EWOD digital microfluidic system. V C 2015 AIP Publishing LLC. [http://dx

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“…In 2012, Wheeler's research team also provided an upside-down cell culture method by creating regions of cell culture on the top cap of a parallel-plate DMF chip [5]. Derek G. et al in 2014 has presented the development of a DMF for the vitrification of mammalian embryos for clinical in vitro fertilization (IVF) applications [6].…”

Section: Introductionmentioning

confidence: 99%

In vitro dynamic fertilization by using EWOD device

Chung

Shen

Chung

et al. 2015

2015 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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This research demonstrates the in vitro fertilization (IVF) technique by using the digital microfluidic (DMF) system. The DMF device has been proved with biocompatibility and used in this research for mouse gametes in vitro fertilization and embryos culture based on the dispersed droplet form. Dynamically moving, cell culture and observation can be achieved by the time-lapse microscopy in an incubator. The fertilization rate of IVF on DMF is 34.8%, and about 25% inseminated embryos dynamically cultured on DMF chip could develop into 8-cell stage. The result indicated that the DMF system has the potential used for assisted reproductive technology.

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Automated vitrification of mammalian embryos on a digital microfluidic device

Cited by 5 publications

References 7 publications

Microfluidics: The future of microdissection TESE?

Microfluidics: The future of microdissection TESE?

Improving the dielectric properties of an electrowetting-on-dielectric microfluidic device with a low-pressure chemical vapor deposited Si3N4 dielectric layer

In vitro dynamic fertilization by using EWOD device

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