In vitro dynamic fertilization by using EWOD device

Abstract

This research demonstrates the in vitro fertilization (IVF) technique by using the digital microfluidic (DMF) system. The DMF device has been proved with biocompatibility and used in this research for mouse gametes in vitro fertilization and embryos culture based on the dispersed droplet form. Dynamically moving, cell culture and observation can be achieved by the time-lapse microscopy in an incubator. The fertilization rate of IVF on DMF is 34.8%, and about 25% inseminated embryos dynamically cultured on DMF chip could develop into 8-cell stage. The result indicated that the DMF system has the potential used for assisted reproductive technology.