Automated study on kinetics and biosensing of glow-type luminescence reaction via digital microfluidics–chemiluminescence

Abstract

Automated manipulation of discrete droplets by digital microfluidics (DMF) combined with chemiluminescence (CL) is promising to achieve automated and sensitive biosensing and bioanalysis.

High sensitivity and automatic chemiluminescence detection of glucose and lactate using a spin-disc paper-based device

“…They can be classified based on the flow control mechanisms into flow injection devices with the differential pressure flow mode, 15 microfluidic paper-based analytical devices (μPADs) driven by capillary force, 16 microchip capillary electrophoresis (MCE) devices using electrokinetic flow, 17 and digital microfluidics (DMF) employing electric fields for droplet control. 18 μPADs have been widely exploited to detect glucose, 19-21 lactate, 16,22 or H 2 O 2 23 using CL due to their advantages in fluid flow control, low cost, and disposability. The capability in fluid flow enabled by the capillary force facilitated the easy control of multiple reagents and reaction sequences on paper devices.…”

“…25 However, the chemiluminescence reaction exhibits flashtype kinetic characteristics, where emitted light sharply increases to its maximum and then rapidly decreases within less than one second. 18 The above delay method was limited in rapid sample introduction since the lateral flow requires time to reach the next reaction region. Therefore, quick contact between the oxidation products and the following reagents for chemiluminescence reading is critical.…”

“…They can be classified based on the flow control mechanisms into flow injection devices with the differential pressure flow mode, 15 microfluidic paper-based analytical devices (μPADs) driven by capillary force, 16 microchip capillary electrophoresis (MCE) devices using electrokinetic flow, 17 and digital microfluidics (DMF) employing electric fields for droplet control. 18…”

Review—Trends on the Development of Non-Enzymatic Electrochemical Sensors Modified with Molecularly Imprinted Polymers for the Quantification of Glucose

“…25 Therefore, it is necessary a continuous glucose monitoring in physiological fluids to avoid complications related to anormal levels of glycemia. In this sense, spectrophotometry, [26][27][28] chromatography, [29][30][31] electrophoresis, 32,33 fluorescence, 34,35 and chemiluminescence 36,37 have been reported as traditional analytical techniques for glucose quantification; unfortunately, these ones need sample pretreatment, sophisticated instrumentation, and the use of excessive organic solvents. 38,39 Methodologies based on electrochemistry represent a viable alternative in the quantification of glucose due to their simplicity, low-cost, rapid response, and easy implementation, overcoming the drawbacks mentioned above.…”