Automated solid-phase organic synthesis in micro-plate wells. Synthesis of N-(alkoxy-acyl)amino alcohols

Search for optimal coupling reagent in multiple peptide synthesizer

“…The plate was capped and shaken on the plate shaker for 3 h. The suspension was transferred by a multichannel pipettor to filter plate (Orochem Technologies, Lombard, IL, USA). The eluate in the deep well plate (VWR) was precipitated by ether (600 L); after standing in the refrigerator for 3 h, the pellet was formed by centrifugation, the supernatant removed by a surface suction device, 3,18 and the pellet was resuspended in ether (600 L) and centrifuged again. The process of supernatant removal and resuspension was repeated three times.…”

Practical Aspects of Combinatorial Solid‐Phase Synthesis

“…In an alternative procedure, the resin beads are allowed to settle and the solvent above the resin bed is aspirated using an aspirator. 134 Solvent delivery into 96 wells is carried out using a 96-well dispenser (www.torviq.com).…”

“…Multiple synthesis in a continuous flow manner was also later reported. [134][135][136][137] For continuous peptide synthesis, a multiple automated robotic synthesizer (MARS) was designed and constructed by Selectide Corporation. 138 Its main feature was a timing procedure for handling multiple synthetic tasks that eliminated any unnecessary respite time and kept the robot arm continuously in operation.…”

Semi-automated high throughput combinatorial solid-phase organic synthesis

“…N-(Alkoxyacyl)amino acids were synthesized as previously described 16 (Scheme 1). The first two combinatorial steps were performed on 12 domino blocks, each housing 12 syringes (144 reaction vessels).…”

“…Our concept is based on the "split only" strategy using parallel synthesis in domino blocks 15 and micro plates. 16 …”