Semi-automated high throughput combinatorial solid-phase organic synthesis

Krchňák, Viktor

doi:10.1002/(sici)1097-0290(1998)61:3<135::aid-cc1>3.0.co;2-s

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What Is Combinatorial Chemistry1

Bis-heterocycles I1

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2011

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Cited by 7 publications

(2 citation statements)

References 18 publications

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“…5,6,32,33 A hybrid approach is the split-and-split technique. 34,35 Although combinatorial chemistry may be applied to a variety of areas of chemistry, to date, the majority of practical applications have been dedicated to drug discovery projects. Combinatorial chemistry is undoubtedly an efficient method for the preparation of compounds in a variety of library sizes.…”

Section: What Is Combinatorial Chemistrymentioning

confidence: 99%

Practical Aspects of Combinatorial Solid‐Phase Synthesis

Hlaváč¹,

Soural²,

Krchňák³

2011

Solid‐Phase Organic Synthesis

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Section: What Is Combinatorial Chemistrymentioning

confidence: 99%

Practical Aspects of Combinatorial Solid‐Phase Synthesis

Hlaváč¹,

Soural²,

Krchňák³

2011

Solid‐Phase Organic Synthesis

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“…The library of bis-heterocycles I was synthesized using the split-split approach23 using polypropylene syringes as reaction vessels on a manually operated Domino Block synthesizer 24. A full library of 2×2×2×2×2 = 32 compound using amino alcohols A1 and A2 , arylfluorides F1 and F2 , four spacers, haloketones H1 and H9 , and acids C2 and C4 was synthesized.…”

Section: Bis-heterocycles Imentioning

confidence: 99%

Combinatorial Libraries of Bis-heterocyclic Compounds with Skeletal Diversity

2008

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Screening for peptide affinity ligands on CIM monoliths

Pflegerl

Podgornik

Berger

et al. 2002

Biotech & Bioengineering

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Screening of peptide ligands for affinity chromatography usually involves incubation with the target protein in a batch system. In an additional step, peptides with fast binding kinetics have to be selected in respect to satisfactory performance under flow conditions on a support ensuring optimal three-dimensional presentation of the peptide. We have developed a rapid screening system based on peptide synthesis and screening on CIM((R)) disks. The disk size was minimized to fit into microplates usually applied for solid-phase extraction. In combination with a vacuum manifold, semi-automated peptide synthesis and screening for binding to a target protein under simulated chromatography conditions are possible. Various analytical methods can be applied for parallel and automated determination of the quantity, integrity, or activity of the target protein in the flow through or bound to the affinity support. This system also allows parallel screening for suitable chromatographic conditions like running buffer, washing, and elution conditions.

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Semi-automated high throughput combinatorial solid-phase organic synthesis

Cited by 7 publications

References 18 publications

Practical Aspects of Combinatorial Solid‐Phase Synthesis

Practical Aspects of Combinatorial Solid‐Phase Synthesis

Combinatorial Libraries of Bis-heterocyclic Compounds with Skeletal Diversity

Screening for peptide affinity ligands on CIM monoliths

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