Automated sample preparation facilitated by PhyNexus MEA purification system for oligosaccharide mapping of glycoproteins

Prater, Bradley D.; Anumula, Kalyan R.; Hutchins, Jeff

doi:10.1016/j.ab.2007.07.011

Cited by 30 publications

(22 citation statements)

References 15 publications

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“…Since MCC has similar properties to cotton, it would be more suitable than DPA-6S for the clean-up of biological samples that were directly derived after digestion. A previously reported labeling method was used in this study after digestion of glycoproteins [27], [32]. Labeling reagent was directly added to the released N -glycan samples that contained detergent, buffer and PNGase F. In addition to the above advantage, MCC is also easy to obtain and handle.…”

Section: Resultsmentioning

confidence: 99%

Purification of Derivatized Oligosaccharides by Solid Phase Extraction for Glycomic Analysis

Zhang

Feng

et al. 2014

PLoS ONE

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Profiling of glycans released from proteins is very complex and important. To enhance the detection sensitivity, chemical derivatization is required for the analysis of carbohydrates. Due to the interference of excess reagents, a simple and reliable purification method is usually necessary for the derivatized oligosaccharides. Various SPE based methods have been applied for the clean-up process. To demonstrate the differences among these methods, seven types of self-packed SPE cartridges were systematically compared in this study. The optimized conditions were determined for each type of cartridge and it was found that microcrystalline cellulose was the most appropriate SPE material for the purification of derivatized oligosaccharide. Normal phase HPLC analysis of the derivatized maltoheptaose was realized with a detection limit of 0.12 pmol (S N−1 = 3) and a recovery over 70%. With the optimized SPE method, relative quantification analysis of N-glycans from model glycoproteins were carried out accurately and over 40 N-glycans from human serum samples were determined regardless of the isomers. Due to the high stability and sensitivity, microcrystalline cellulose cartridge showed potential applications in glycomics analysis.

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Section: Resultsmentioning

confidence: 99%

Purification of Derivatized Oligosaccharides by Solid Phase Extraction for Glycomic Analysis

Zhang

Feng

et al. 2014

PLoS ONE

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“…Polyamide S6 and nylon filter disks have been used for 2-aminobenzoic acid (2-AA, 16) derivatized carbohydrates [133], Oasis HLB cartridges from Waters have been employed for glycans derivatized with 3-(acetylamino)-6-aminoacridine (AA-Ac, 17) or 2-aminoacridone (AMAC, 18) and graphitized carbon has been used for carbohydrates labelled with 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS, 19) [134] (further discussion of these specific derivatives is presented below). Gel filtration has been favoured by Hase and co-workers, [135] and phenol/chloroform extraction is one of the more unusual methods [136].…”

Section: Methodsmentioning

confidence: 99%

Derivatization of carbohydrates for analysis by chromatography; electrophoresis and mass spectrometry

Harvey¹

2011

Journal of Chromatography B

217

156

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“…Commonly utilized purification strategies are SPE with different stationary phase materials (e.g. C18, HILIC, and PGC), paper chromatography (Bigge et al, 1995), filtration (Hase, 1994; Prater, Anumula, & Hutchins, 2007), dialysis and precipitation (Pabst, Kolarich, Poltl, Dalik, Lubec, Hofinger et al, 2009). Unfortunately, the introduction of an additional purification step reduces sample preparation throughput and creates the potential for sample loss.…”

Section: 2 Sample Handling In Glycomicsmentioning

confidence: 99%

Quantitative Glycomics

Veillon

Zhou

Mechref

2017

Methods in Enzymology

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Glycosylation is one of the most common and essential protein modifications. Glycans conjugated to biomolecules modulate the function of such molecules through both direct recognition of glycan structures and indirect mechanisms that involve the control of protein turnover rates, stability, and conformation. The biological attributes of glycans in numerous biological processes and implications in a number of diseases highlight the necessity for comprehensive characterization of protein glycosylation. This chapter review cutting-edge-methods and tools developed to facilitated quantitative glycomics. The chapter highlight the different methods employed for the release and purification of glycans from biological samples. The most effective labeling methods developed for sensitive quantitative glycomics are also described and discussed. The chromatographic approaches that have been used effectively in glycomics are also highlighted.

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Automated sample preparation facilitated by PhyNexus MEA purification system for oligosaccharide mapping of glycoproteins

Cited by 30 publications

References 15 publications

Purification of Derivatized Oligosaccharides by Solid Phase Extraction for Glycomic Analysis

Purification of Derivatized Oligosaccharides by Solid Phase Extraction for Glycomic Analysis

Derivatization of carbohydrates for analysis by chromatography; electrophoresis and mass spectrometry

Quantitative Glycomics

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