Automated paper-based devices by microfluidic timing-valve for competitive ELISA

Lai, Yu-Ting; Tsai, Jing‐Fa; Hsu, Jin‐Chen; Lu, Yen‐Wen

doi:10.1109/memsys.2017.7863663

Cited by 6 publications

(8 citation statements)

References 11 publications

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“…However, these valves required long response times and large volumes of actuation fluids. Lai et al [16] provided a design for a timing valve in their device that consisted of a surfactant and a wax barrier to provide appropriate time delays to sequentially handle the multiple fluidic operations of the device. Koo et al [17] used an electrowetting valve in which a dielectric material that is normally hydrophobic, is polarized and becomes hydrophilic when the valve is actuated with an applied potential difference.…”

Section: Introductionmentioning

confidence: 99%

Numerical and Experimental Modeling of Paper-Based Actuators

Kumar

Heidari-Bafroui

Faghri

et al. 2021

The 1st International Electronic Conference on Chemical Sensors and Analytical Chemistry

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Microfluidic paper-based analytical devices (μPADs) have witnessed a great extent of innovation over the past decade, developing new components and materials assisting the diagnosis of different diseases and sensing of a wide range of biological, chemical, optical, and electrochemical phenomena. The novel paper-based cantilever (PBC) actuator is one the major components that allows autonomous loading and control of multiple fluid reagents required for the accurate operation of paper-based microfluidic devices. This paper provides an extensive overview of numerical and experimental modeling of fluidically controlled PBC actuators for automation of the paper-based assay. The PBC model undergoing hygro-expansion utilizes quasi-static 2D fluid loaded structure governed by the Euler–Bernoulli beam theory for small and moderately large deflections. The solution for the model can avail the response of paper-based actuators for response deflection θ, within 0° to 10° under the assumption of insignificant cross-sectional deformation. The actuation of PBC obtained using a quasi-static theory shows that our results are consistent with quantitative experiments demonstrating the adequacy of models.

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Section: Introductionmentioning

confidence: 99%

Numerical and Experimental Modeling of Paper-Based Actuators

Kumar

Heidari-Bafroui

Faghri

et al. 2021

The 1st International Electronic Conference on Chemical Sensors and Analytical Chemistry

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“…Wax printing [60][61][62][63][64] Whatmann CHR # 1 Assembly of paper in pre-cut lamination sheets [65] PHB fibers Electrospinning and dip-coating with poly(MMA-co-MAA) [5] PHBV fibers [6] Nylon Dip-coating with poly(MMA-co-MAA) [37] Cellulose Photolithography [66] Filter paper Whatman # 42 Commercialized material with proprietary fabrication method~1 cm diameter circles cut into pendent disk shapes to avoid cross contamination [67] Whatman Fusion 5 TM paper Square shaped layers with surface area of 3 cm 2 [68,69] NC Wax printing µPAD with printed channels that function as timing valves [70] Inkjet printing [71] Whatmann CHR # 1 Flexographic printing Polystyrene printed paper (297 mm × 105 mm) with hydrophobic barriers [22] Polyester-backed paper, Whatmann CHR # 1, NC membrane Computer-controlled knife cutting Star, candelabra, and other structures [23] Aluminum foil-baked Whatmann CHR # 1 CO 2 laser cutting/engraving µPAD design with very small features and narrow barriers [72] Whatman CHR # 1 coated with functional polymers/copolymers Vapor-phase polymer deposition Integration of multiple advanced unit operations onto a single µPAD device [73] Whatman # 4 loaded with PEI microcapsules, BCIN, and NBT Suspension mixing followed by filtration Paper strips [74] Nylon # 6 fibers blended with PSBMA and PAA Electrospinning blended fiber deposition on a glass slip [50] PVDF nano-fiber membrane Western blotting platforms (6 cm × 8 cm) [52] Nylon # 6 fibers with Cu-Au nanoparticles Paper strips [75] PCL fiber membrane Folded and pressed sheet of membrane (25 mm × 40 mm) [51] Nomenclature: PHB = Poly(3-hydroxybutyrate; poly(MMA-co-MAA) = poly methylmetacrylate-co-methacrylic acid PHBV = Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); NC = nitrocellulose; PEI = poly(ethyleneimine); BCIN = 5-bromo-4-chloro-3-indolyl-α-D-N-acetylneuraminic acid; NTB = nitro blue tetrazolium; PVDF = Polyvinylidene fluoride; Cu-Au: Copper-gold; PCL = Poly(ε-caprolactone). CHR = chromatography.…”

Section: Whatman #mentioning

confidence: 99%

“…Limited to tests with a low number of false negative outputs [63] Reduced sample volume, higher sensitivity, shorter assay time, and lower fabrication cost Low stability and short shelf life, which are typical drawbacks of the wax printing technique [64] Equipped with timing valve that provides an opportunity for multiple-step performance in assay procedure Similar discrimination capability than conventional ELISA without any improvement was reported; the method has to be further assessed [70] Inkjet printing technique was applied to fabricate a µPAD equipped with timing valves for hCG detection Simple, straightforward, and low cost fabrication technique by using inkjet printing…”

Section: Whatman # 1 Mimicked 96-well Plate For Extracellular Vesiclementioning

confidence: 99%

“…Detection via smart devices has rapidly penetrated the worldwide market introducing tremendous opportunities for the on-site processing of data, instead of sample transfer to a centralized clinical facility (Table 2) [2,[57][58][59][60][61][62][63][64][65][66][67][68][69][70][71]128,139]. With their storage capacity, smart devices serve clinical practice well, enabling information to be collected and stored to a much greater extent than previously possible.…”

Section: Readout Outcomesmentioning

confidence: 99%

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Paper and Fiber-Based Bio-Diagnostic Platforms: Current Challenges and Future Needs

2017

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Abstract:In this perspective article, some of the latest paper and fiber-based bio-analytical platforms are summarized, along with their fabrication strategies, the processing behind the product development, and the embedded systems in which paper or fiber materials were integrated. The article also reviews bio-recognition applications of paper/fiber-based devices, the detected analytes of interest, applied detection techniques, the related evaluation parameters, the type and duration of the assays, as well as the advantages and disadvantages of each technique. Moreover, some of the existing challenges of utilizing paper and/or fiber materials are discussed. These include control over the physical characteristics (porosity, permeability, wettability) and the chemical properties (surface functionality) of paper/fiber materials are discussed. Other aspects of the review focus on shelf life, the multi-functionality of the platforms, readout strategies, and other challenges that have to be addressed in order to obtain reliable detection outcomes.

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“…Experiments were conducted at least three times for each concentration of both surfactants. (a) is reproduced with permission from reference[32].…”

mentioning

confidence: 99%

Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA

et al. 2019

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Paper-based technologies have been drawing increasing attentions in the biosensor field due to their economical, ecofriendly, and easy-to-fabricate features. In this paper, we present a time-delay valve mechanism to automate a series of procedures for conducting competitive enzyme-linked immunosorbent assay (ELISA) on a paper-based device. The mechanism employs a controllable time-delay valve, which has surfactants to dissolve the hydrophobic barriers, in a fluid pathway. The valves can regulate the liquid and sequentially deliver the sample flow for automating ELISA procedures in microchannels. Competitive ELISA is achieved in a single step once the sample, or small molecule pesticide (e.g., Imidacloprid), is applied onto the paper-based device with a comparable sensitivity to plate-based competitive ELISA. The results further demonstrate the appositeness of using paper-based devices with the valve designs for on-the-go ELISA detection in agriculture and biomedical applications.

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Automated paper-based devices by microfluidic timing-valve for competitive ELISA

Cited by 6 publications

References 11 publications

Numerical and Experimental Modeling of Paper-Based Actuators

Numerical and Experimental Modeling of Paper-Based Actuators

Paper and Fiber-Based Bio-Diagnostic Platforms: Current Challenges and Future Needs

Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA

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