Current microfluidic paper-based devices lack crucial components for fluid manipulation. We created a fluidic diode fabricated entirely on a single layer of paper to control the wicking of fluids. The fluidic diode is a two-terminal component that promotes or stops wicking along a paper channel. We further constructed a trigger and a delay valve based on the fluidic diode. Furthermore, we demonstrated a high-level functional circuit, consisting of a diode and a delay valve, to manipulate two fluids in a sequential manner. Our study provides new, transformative tools to manipulate fluid in microfluidic paper-based devices.
Currently, the Lateral flow Immunoassays (LFIAs) are not able to perform complex multi-step immunodetection tests because of their inability to introduce multiple reagents in a controlled manner to the detection area autonomously. In this research, a point-of-care (POC) paper-based lateral flow immunosensor was developed incorporating a novel microfluidic valve technology. Layers of paper and tape were used to create a three-dimensional structure to form the fluidic network. Unlike the existing LFIAs, multiple directional valves are embedded in the test strip layers to control the order and the timing of mixing for the sample and multiple reagents. In this paper, we report a four-valve device which autonomously directs three different fluids to flow sequentially over the detection area. As proof of concept, a three-step alkaline phosphatase based Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol with Rabbit IgG as the model analyte was conducted to prove the suitability of the device for immunoassays. The detection limit of about 4.8 fm was obtained.
This paper presents an experimental investigation of laminar gas flow through microchannels. The independent variables: relative surface roughness, Knudsen number and Mach number were systematically varied to determine their influence on the friction factor. The microchannels were etched into silicon wafers, capped with glass, and have hydraulic diameters between 5 and 96 μm. The pressure was measured at seven locations along the channel length to determine local values of Knudsen number, Mach number and friction factor. All measurements were made in the laminar flow regime with Reynolds numbers ranging from 0.1 to 1000. The results show close agreement for the friction factor in the limiting case of low Ma and low Kn with the incompressible continuum flow theory. The effect of compressibility is observed to have a mild (8 percent) increase in the friction factor as the Mach number approaches 0.35. A 50 percent decrease in the friction factor was seen as the Knudsen number was increased to 0.15. Finally, the influence of surface roughness on the friction factor was shown to be insignificant for both continuum and slip flow regimes.
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