Automated collection of double red blood cell units with a variable‐volume separation chamber

Abstract

BACKGROUND: Automated collection of blood components offers multiple advantages and has prompted development of portable devices. This study sought to document the biochemical and hematologic properties and in vivo recovery of red cells (RBCs) collected via a new device that employed a variable-volume centrifugal separation chamber. STUDY DESIGN AND METHODS:Normal subjects (n = 153) donated 2 units of RBCs via an automated blood collection system (Cymbal, Haemonetics). Procedures were conducted with wall outlet power (n = 49) or the device's battery source (n = 104). Units were collected with or without leukoreduction filtration and were stored in AS-3 for 42 days. The units were assessed via standard biochemical and hematologic tests before and after storage, and 24 leukoreduced (LR) and 24 non-LR RBCs were radiolabeled on Day 42 with Na2

Double red cell concentrates -in vitro quality after delayed refrigeration

“…A number of different automated devices are capable of collecting red cells, either alone or concurrently with other blood components, and a number of studies on this have been previously reported (Greenwalt et al , 2002; Snyder et al , 2003; Picker et al , 2007; AuBuchon et al , 2008). A comprehensive study of the quality of leucoreduced RCC collected using the Amicus and Alyx separators (Fenwal, Deerfield, IL, USA), the MCS+ (Haemonetics, Braintree, MA, USA) and the Trima (CaridianBCT, Lakewood, CO, USA) found that the RCC met all regulatory requirements during their storage period (Picker et al , 2007).…”

“…The Cymbal (Haemonetics) is a portable automated red cell collection device that allows the separation of variable volumes of blood in its centrifuge chamber. Leucoreduced RCC collected by the Cymbal using CP2D anticoagulant and stored in AS‐3 optimal additive solution have been shown to be of acceptable quality , as judged by in vitro analysis and in vivo recovery (AuBuchon et al , 2008) , but there are no published reports on the quality of RCC collected using the Cymbal with citrate, phosphate and dextrose (CPD‐50) anticoagulant and stored in saline, adenine, glucose and mannitol (SAGM) optimal additive solution, which is currently used for the collection of WB‐derived RCC in the United Kingdom. It was therefore necessary to conduct an investigation of the quality of Cymbal RCC stored in SAGM.…”

Clinical and Quality Evaluation of Red Blood Cell Units Collected Via Apheresis Versus Those Obtained Manually

“…7 Mean percentages of hemolysis for stored ARBC, as reported in the literature, ranged from .05% to .8%. 1,7,[10][11][12] This variation may likely be related to the different apheresis machines and to the different additive solutions that various study groups used.…”

Rejuvenation capacity of red blood cells in additive solutions over long‐term storage

“…Total Hb (Sysmex XE2100, Sysmex, Mundelein, IL) and spun hematocrit were determined for each tube. Protein was extracted from each tube with 8% perchloric acid method as previously described 16 . The extracted supernatants were frozen at less than −75°C until assayed.…”

“…Protein was extracted from each tube with 8% perchloric acid method as previously described. 16 The extracted supernatants were frozen at less than -75°C until assayed. The neutralized perchloric acid extracts were analyzed for ATP using the NADP+ reduction method of Beutler 17 automated for the Cobas Fara.…”