Automated cervical precancerous cells screening system based on Fourier transform infrared spectroscopy features

Abstract

Fourier transform infrared (FTIR) spectroscopy technique can detect the abnormality of a cervical cell that occurs before the morphological change could be observed under the light microscope as employed in conventional techniques. This paper presents developed features extraction for an automated screening system for cervical precancerous cell based on the FTIR spectroscopy as a second opinion to pathologists. The automated system generally consists of the developed features extraction and classification stages. Signal processing techniques are used in the features extraction stage. Then, discriminant analysis and principal component analysis are employed to select dominant features for the classification process. The datasets of the cervical precancerous cells obtained from the feature selection process are classified using a hybrid multilayered perceptron network. The proposed system achieved 92% accuracy.

Investigation of human pancreatic cancer tissues by Fourier Transform Infrared Hyperspectral Imaging

“…By hyperspectral imaging of specific areas of tissue samples, it is possible to achieve a morpho‐chemical correlation between histological and vibrational analysis . The potential offered by FTIR‐HSI to obtain relevant and punctual biomolecular information at molecular level and to detect changes in the biochemical composition and conformation of the most relevant biomolecules on the same sample and at the same time has been already reported in previous studies , investigating prostate , cervix , breast , colon , lung , thyroid and skin tumoral lesions. In some cases, the FTIR‐HSI approach played a key role in detecting early stages of inflammatory and malignant diseases, helping to gain a deeper understanding of the etiologic nature of different pathologies, even before any detectable morphological evidence .…”

Clinical applications of infrared and Raman spectroscopy in the fields of cancer and infectious diseases

“…Three different spectroscopic studies using single cells from LBC samples investigated cervical precancer of low and high grades [64][65][66] . One study highlighted that cytological samples were suitable for analysis of fresh samples or after storage at À25 o C, but not À80 o C [64] .…”

“…After comparison of controls with high-grade precancer across all three studies, the sensitivity and specificity were found to be between 86-100% and 90-100% respectively; sensitivity and specificity for detecting low-grade lesions were 94% and 95-98% respectively. The authors proposed an automated screening system for cervical cancer [65] and also highlighted that histological assessment provided higher diagnostic accuracy in comparison to cytological assessment [66] .…”

Rapid intra‐operative diagnosis of kidney cancer by attenuated total reflection infrared spectroscopy of tissue smears

“…Alternatively to the spectral histopathology of tissue sections, spectral cytopathology was carried out for cancer detection in cells exfoliated from oral, nasopharyngeal cavities, the esophagus, the bladder and the cervix [25][26][27]. Such approach does not involve the freeze-drying procedure used for the tissue sections and employs fixatives (typically, solutions of alcohols) with minimal adverse influence [28,29].…”