Automated assembly of microfluidic "lab-on-a-disc"

Berger, Marvin; Müller, Tobias; Voebel, Toni C.; Baum, Christoph; Glennon, Thomas; Mishra, Rohit; Kinahan, David J.; King, Damien; Ducrée, Jens; Brecher, Christian

doi:10.1117/12.2290348

Cited by 4 publications

(2 citation statements)

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“…Centrifugal microfluidic discs are able to perform multiple steps and are utilized in clinical chemistry, immunodiagnostics, cell handling, and molecular diagnostics, as well as in food, water, and soil analysis [12]. To facilitate the scale-up of such technologies, integrated systems and mass-manufacturing methods have also been implemented [14], thus allowing for high commercialization potential [15][16][17]. Most blood-related lab-on-a-disc devices are developed for the purpose of preparing cell-free plasma [18].…”

Section: Introductionmentioning

confidence: 99%

An Automated Centrifugal Microfluidic Platform for Efficient Multistep Blood Sample Preparation and Clean-Up towards Small Ion-Molecule Analysis

Hou,

Mishra,

Zhao

et al. 2023

Micromachines

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Sample preparation for mass spectroscopy typically involves several liquid and solid phase clean-ups, extractions, and other unit operations, which are labour-intensive and error-prone. We demonstrate a centrifugal microfluidic platform that automates the whole blood sample’s preparation and clean-up by combining traditional liquid-phase and multiple solid-phase extractions for applications in mass spectroscopy (MS)-based small molecule detection. Liquid phase extraction was performed using methanol to precipitate proteins in plasma separated from a blood sample under centrifugal force. The preloaded solid phase composed of C18 beads then removed lipids with a combination of silica particles, which further cleaned up any remaining proteins. We further integrated the application of this sample prep disc with matrix-assisted laser desorption/ionization (MALDI) MS by using glancing angle deposition films, which further cleaned up the processed sample by segregating the electrolyte background from the sample salts. Additionally, hydrophilic interaction liquid chromatography (HILIC) MS was employed for detecting targeted free amino acids. Therefore, several representative ionic metabolites, including several amino acids and organic acids from blood samples, were analysed by both MALDI-MS and HILIC-MS to demonstrate the performance of this sample preparation disc. The fully automated blood sample preparation procedure only took 35 mins, with a throughput of three parallel units.

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Section: Introductionmentioning

confidence: 99%

An Automated Centrifugal Microfluidic Platform for Efficient Multistep Blood Sample Preparation and Clean-Up towards Small Ion-Molecule Analysis

Hou,

Mishra,

Zhao

et al. 2023

Micromachines

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“…A critical limitation of event-triggered valving is that timing of their actuation depends on the dissolution time of the DFs and intervals for liquid transfer about the disc. While their dissolution time can be tailored by their formulation [ 62 , 63 , 64 ] and even used for reagent storage [ 65 , 66 ], resorting to commercially available (single-grade) DFs in microfluidic devices is beneficial toward mass manufacture [ 67 ]. To control timing of event-triggered valves, researchers have focused on alternative mechanisms such as hybrid event-triggered valves that are opened using supporting instrumentation [ 61 ].…”

Section: Introductionmentioning

confidence: 99%

Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves

et al. 2021

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Within microfluidic technologies, the centrifugal microfluidic “Lab-on-a-Disc” (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize ‘wet bench’ laboratory protocols. We present the combination of ‘event-triggered dissolvable film valves’ with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL−1 and limit of quantitation (LoQ) of 135.87 ng mL−1.

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