Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water

Heub, Sarah; Tscharner, Noe; Monnier, V.; Kehl, Florian; Dittrich, Petra S.; Follonier, S.; Barbe, Laurent

doi:10.1016/j.chroma.2014.12.076

Cited by 20 publications

(11 citation statements)

References 32 publications

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“…Moreover, the instrument has a small footprint (25 cm x 15 cm x 10 cm) and low-cost compared to typical manual or automated SPE setups. 13 If needed, the throughput for multiple sample analysis can therefore be increased by using a few devices in parallel. Another possibility would be to design a method for smaller volumes of sample and eluent, which would reduce the time needed for the procedure.…”

Section: Discussionmentioning

confidence: 99%

“…Automation of the procedure helps to reduce human-related errors. In our previous work 13 we introduced our prototype for automated SPE, and our method was applied to the analysis of the natural hormone 17β-estradiol in sea water samples. With the present video we would like to highlight the technical advantages of our method compared to traditional off-line and on-line SPE, and its particular compatibility with detection by immuno-reactions.…”

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confidence: 99%

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A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Heub

Tscharner

Kehl

et al. 2016

JoVE

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A new method for solid phase extraction (SPE) of environmental water samples is proposed. The developed prototype is cost-efficient and user friendly, and enables to perform rapid, automated and simple SPE. The pre-concentrated solution is compatible with analysis by immunoassay, with a low organic solvent content. A method is described for the extraction and pre-concentration of natural hormone 17β-estradiol in 100 ml water samples. Reverse phase SPE is performed with octadecyl-silica sorbent and elution is done with 200 µl of methanol 50% v/v. Eluent is diluted by adding di-water to lower the amount of methanol. After preparing manually the SPE column, the overall procedure is performed automatically within 1 hr. At the end of the process, estradiol concentration is measured by using a commercial enzyme-linked immune-sorbent assay (ELISA). 100-fold pre-concentration is achieved and the methanol content in only 10% v/v. Full recoveries of the molecule are achieved with 1 ng/L spiked de-ionized and synthetic sea water samples. Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Heub

Tscharner

Kehl

et al. 2016

JoVE

Self Cite

View full text Add to dashboard Cite

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“…Therefore, it is vital to establish a sensitive and reliable method for the E2 detection [11]. Liquid chromatography and/or gas chromatography/mass spectrometry [12,13] and HPLC have been used for the detection of E2. However, these chromatographic methods cannot meet requirements in the perspectives of time [14], cost, lower sensitivity and the need for trained people [15].…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted biomimetic surface plasmon resonance sensor for hormone detection

Türkoğlu¹,

Bakhshpour²,

Deni̇zli̇³

2019

Biointerface Res Appl Chem

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In this study, 17β-estradiol (E2) was performed by surface plasmon resonance (SPR) based on molecularly imprinted technique. In the first, SPR sensor was prepared by the formation of E2 imprinted poly(2-hydroxyetyl methacrylate) / (N-methacryloyl-(L)- leucine methyl esters) (PHEMALM) on the modified gold surface of SPR chip. Also, non-imprinted SPR chip was prepared by the same method at the E2 imprinted PHEMALM (E2-MIPHEMALM-SPR) chip without E2. E2-MIPHEMALM-SPR and non-imprinted (NIPHEMALM-SPR) chips were characterized with Fourier transform infrared spectrophotometry (FTIR), ellipsometry, atomic force microscopy (AFM) and contact angle measurements. After that, SPR chips were completed to SPR system to investigate kinetic properties for E2. The sensing ability of E2-MIPHEMALM-SPR chip was investigated with 20-10000 ng/mL concentrations of 17βestradiol solutions. The E2-MIPHEMALM-SPR chip was showed more selectivity for 17β-estradiol than NIPHEMALM-SPR chip. To show the selectivity of E2-MIPHEMALM-SPR chip competitive adsorption of 17β-estradiol, cholesterol and stigmasterol were investigated. E2-MIPHEMALM-SPR chip was investigated ten times with same concentrations of 17β-estradiol solution to show reuse of the chip. The results showed that the E2-MIPHEMALM-SPR chip has high selectivity for 17β-estradiol.

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“…EDCs are introduced into the aquatic environment by human activities, both domestic and industrial . Most EDCs characteristics are persistence in the environment . Thus, they can enter the food chain and adversely affect humans.…”

Section: Introductionmentioning

confidence: 99%

Polypyrrole‐coated alginate/magnetite nanoparticles composite sorbent for the extraction of endocrine‐disrupting compounds

Bunkoed

Nurerk

Wannapob

et al. 2016

J of Separation Science

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Magnetite nanoparticles incorporated into alginate beads and coated with a polypyrrole adsorbent were prepared (polypyrrole/Fe3 O4 /alginate bead) and used as an effective magnetic solid-phase extraction sorbent for the extraction and enrichment of endocrine-disrupting compounds (estriol, β-estradiol and bisphenol A) in water samples. The determination of the extracted endocrine-disrupting compounds was performed using high-performance liquid chromatography with a fluorescence detector. The effect of various parameters on the extraction efficiency of endocrine disrupting compounds were investigated and optimized including the type and amount of sorbent, sample pH, extraction time, stirring speed, and desorption conditions. Under optimum conditions, the calibration curves were linear in the concentration range of 0.5-100.0 μg/L, and the limit of detection was 0.5 μg/L. The developed method showed a high extraction efficiency, the recoveries were in the range of 90.5 ± 4.1 to 98.2 ± 5.5%. The developed sorbent was easy to prepare, was cost-effective, robust, and provided a good reproducibility (RSDs < 5%), and could be reused 16 times. The developed method was successfully applied for the determination of endocrine-disrupting compounds in water samples.

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Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water

Cited by 20 publications

References 32 publications

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Molecularly imprinted biomimetic surface plasmon resonance sensor for hormone detection

Polypyrrole‐coated alginate/magnetite nanoparticles composite sorbent for the extraction of endocrine‐disrupting compounds

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