Automated analysis of vitamin E isomers in vegetable oils by continuous membrane extraction and liquid chromatography–electrochemical detection

Sánchez-Pérez, A.; Delgado-Zamarreño, M.M.; Bustamante-Rangel, Myriam; Hernández-Méndez, J.

doi:10.1016/s0021-9673(00)00314-9

Cited by 44 publications

(27 citation statements)

References 18 publications

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“…However, the concentration of alpha-tocopherol was higher than the value obtained in Costa Rica (69 mg/kg) (Monge-Rojas & Campos, 2011). Regarding the concentration of gamma-tocopherol found in the corn oil, it was verified that this is lower than the concentration found in the literature and similar to that obtained by Monge-Rojas and Campos (2011) (220 mg/kg) (Sánchez-Pérez et al, 2000;Branco, 2009;Franke et al, 2007;Gliszczyńska-Świgło et al, 2007;Kamal-Eldin, 2006;Schwartz et al, 2008;Lima & Gonçalves, 1997;Ramadan & Wahdan, 2012).…”

Section: Discussionsupporting

confidence: 79%

“…However, it was lower than the value found in Egypt (417.0 mg/ kg) (Ramadan & Wahdan, 2012), in the United States (222.1 mg/ kg) (Franke et al, 2007), Brasil (240.0 mg/kg) (Branco, 2009) (Sánchez-Pérez et al, 2000). However, the concentration of alpha-tocopherol was higher than the value obtained in Costa Rica (69 mg/kg) (Monge-Rojas & Campos, 2011).…”

Section: Discussionmentioning

confidence: 54%

“…The control of vitamin E levels in foods is also of great importance to ensure that the daily ingestion is adequate, as this is an essential factor for good health (Sánchez-Pérez et al, 2000). In order to adjust the ingestion of nutrients by the population it is important to know the main food-sources, as well as the most consumed foods (Batista et al, 2007).…”

Section: Determination Of Alpha-and Gamma-tocopherol Using High Perfomentioning

confidence: 99%

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Alpha-tocopherol and gamma-tocopherol concentration in vegetable oils

et al. 2014

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 54%

Section: Determination Of Alpha-and Gamma-tocopherol Using High Perfomentioning

confidence: 99%

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Alpha-tocopherol and gamma-tocopherol concentration in vegetable oils

et al. 2014

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“…α-Tocopherol is absent in sesame oil and δ-tocopherol in olive oil. The results obtained are generally in agreement with the literature data (Berasategi et al, 2012;Rammell and Hoogenboom, 1985;Sanchez-Perez et al, 2000). Lack of separation of β-and γ-tocopherols in the case of edible oils introduce rather small error in quantification of these isomers (Gliszczynska-Swiglo and Sikorska, 2004).…”

Section: Quantification Of Tocopherols In Edible Plant Oilssupporting

confidence: 89%

Tocopherol Content in Vegetable Oils Using a Rapid HPLC Fluorescence Detection Method

Bele

Matea

Răducu

et al. 2013

Not Bot Hort Agrobot Cluj

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A quick and direct method based on reversed phase high performance liquid chromatography with fluorescence detector for measuring tocopherols (α , β + γ and δ) has been developed. Oils are diluted in methanol: hexane: tetrahydrofuran (neither previous extraction of tocopherols nor saponification procedure are required) and after being vortexed and centrifuged, an aliquot of the overlay was injected directly into an Alltima C 18 column. Acetonitrile and methanol (50: 50) mixture was used as a mobile phase with a flow rate of 1 mL min -1 . Quantification of tocopherols was performed by fluorescence detector at 290 nm excitation wavelength and 325 nm emission wavelength. Tocopherols were separated at 25°C in less than 10 min after injection. The method has good limit of detection (9 ng g -1 for α-tocopherol and 8 ng g -1 for β-, γ-and δ-tocopherols) and reproducibility (CV< 2.9 %). This method can be used to assess the influence of genetic modification of oil seeds on the distribution of tocopherols or the effect of tocopherols on the oxidative stability of edible plant oils.

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“…Derivatization of biophenols is not required in HPLC, which provides faster, and more sensitive, selective and robust methods than GC [9,10,[25][26][27]. Sample preparation in RP-HPLC can be carried out either with saponification followed by extraction to remove the saponificated material [6,[27][28][29][30][31], or without saponification, thus by directly extracting the fraction of interest with an adequate solvent [7], or by using a membrane extractor mounted online with the chromatograph [27].…”

Section: Introductionmentioning

confidence: 99%

Characterization of hydroxyaromatic compounds in vegetable oils by capillary electrophoresis with direct injection in an oil‐miscible KOH/propanol/methanol medium

et al. 2005

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The separation of hydroxyaromatic compounds in vegetable oils, including synthetic antioxidants (3-tert-butyl-4-hydroxyanisol and 2,6-di-tert-butyl-4-hydroxytoluene), E-vitamers and other natural oil components, by nonaqueous capillary electrophoresis in an oil-miscible background electrolyte (BGE) was investigated. The BGE contained 40 mM KOH in a methanol/1-propanol (PrOH) mixture (15:85 v/v). The oil samples were 1:1 diluted with PrOH and directly injected in the capillary. Under negative polarity (cathode at the injection end), the anionic solutes moved faster than the electroosmotic flow, being well-resolved among them and from the triacylglycerols. Using virgin palm, extra virgin olive, wheat germ, virgin soybean and other oils, the capability of the procedure to quickly yield a characteristic profile of the biophenols present in the sample was demonstrated. The alpha-, (beta + gamma)- (as unresolved pair) and delta-tocopherols of a soybean oil sample were quantified.

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Automated analysis of vitamin E isomers in vegetable oils by continuous membrane extraction and liquid chromatography–electrochemical detection

Cited by 44 publications

References 18 publications

Alpha-tocopherol and gamma-tocopherol concentration in vegetable oils

Alpha-tocopherol and gamma-tocopherol concentration in vegetable oils

Tocopherol Content in Vegetable Oils Using a Rapid HPLC Fluorescence Detection Method

Characterization of hydroxyaromatic compounds in vegetable oils by capillary electrophoresis with direct injection in an oil‐miscible KOH/propanol/methanol medium

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