Characterization of hydroxyaromatic compounds in vegetable oils by capillary electrophoresis with direct injection in an oil‐miscible KOH/propanol/methanol medium

Mendonça, Carla Rosane Barboza; Bica, Clara Isméria Damiani; Piatnicki, Clarisse Maria Sartori; Simó‐Alfonso, Ernesto Francisco; Ramis‐Ramos, Guillermo

doi:10.1002/elps.200410394

Cited by 13 publications

(14 citation statements)

References 56 publications

(68 reference statements)

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“…To carry out the different experiments of the Box-Behnken design, olive oil samples spiked with 40 μg mL −1 of oleuropein glucoside and tyrosol, 10 μg mL −1 of vanillic and gentisic acids, and 20 μg mL −1 of the rest of compounds were dissolved with 1-propanol in a 1:1 (v/v) ratio, in accordance with the work previously published by Mendonςa et al [34]. En each case, the conditions, the BGE composition, temperature, and injection time, indicated by the design were used and the UV and FL electropherograms were registered.…”

Section: Methodsmentioning

confidence: 99%

“…The concentration range of phenolic compounds in EVOO is very broad [44], HYTY and TY being two of the most important and major compounds in these samples [12,17,45]. The high content in these compounds allows carrying out their detection by direct injection into the capillary of the sample dissolved in 1-propanol, which others authors have previously performed [34]. Figure 6A shows the electropherograms corresponding to an olive oil/1-propanol 1:1 (v/v) mixture obtained in the previously optimized conditions.…”

Section: Identification Of the Peaks: Validationmentioning

confidence: 99%

“…Response surface methodology for the optimization of several injection and separation variables According to Mendonça et al [34], the presence of 1-propanol in the BGE enables the direct injection of EVOO into the capillary. In accordance with our previous studies, a higher percentage of 1-propanol in a BGE consisting of 1-propanol and methanol as solvents provided lower separation times.…”

Section: Selection Of the Cleaning Proceduresmentioning

confidence: 99%

“…These authors used a BGE containing malonate in methanol and, finally, proved that it is possible to determine some analytes without tedious pretreatment of the samples. In 2005, Mendonça et al [34] carried out the characterization of hydroxyaromatic compounds in vegetable oils using a BGE containing 40 mM KOH in a methanol/ 1-propanol mixture (15:85, v/v). The importance of this paper relies in the pretreatment of the sample that can be reduced since, in some cases, the oil samples were 1:1 diluted with 1-propanol and directly injected in the capillary.…”

Section: Introductionmentioning

confidence: 99%

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Development of a non-aqueous capillary electrophoresis method with UV–visible and fluorescence detection for phenolics compounds in olive oil

et al. 2012

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Response surface methodology has been applied to the optimization of a simple and rapid non-aqueous capillary electrophoresis method for the separation and determination of several phenolic compounds belonging to the different families present in olive oil. A Box-Behnken design was employed and a total of 27 experiments were performed using olive oil samples spiked with the phenols and injected directly in the capillary after dilution 1:1 with 1-propanol. Finally, the background electrolyte (BGE) was constituted of 25 mM boric acid and 18 mM KOH in a mixture of 74:26 (v/v) 1-propanol/methanol. The hydrophobicity of the BGE allows its miscibility with the olive oil and, as a consequence, the possibility of characterizing and determining these kinds of compounds in this sample without any pretreatment. A hydrodynamic injection (6 s, -30 mbar) was applied and the separation was carried out using 35 °C and +20 kV of separation temperature and voltage, respectively. A capillary with two detection windows for serial online UV and fluorescence detection was satisfactorily employed. The validation of the method was carried out by setting the calibration curves, and the figures of merit were finally obtained. A lineal relationship between the corrected peak area and concentration and limits of detection in the order of micrograms per milliliter were found.

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Section: Methodsmentioning

confidence: 99%

Section: Identification Of the Peaks: Validationmentioning

confidence: 99%

Section: Selection Of the Cleaning Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of a non-aqueous capillary electrophoresis method with UV–visible and fluorescence detection for phenolics compounds in olive oil

et al. 2012

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“…The analytical applications of NACE have been principally focused on pharmaceutical and environmentally important analytes, although NACE methodologies have been also used successfully to determine compounds like phenols 17–21, theaflavins 22, benzoic and sorbic acids 23.…”

Section: Introductionmentioning

confidence: 99%

NACE‐ESI‐TOF MS to reveal phenolic compounds from olive oil: Introducing enriched olive oil directly inside capillary

2009

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Most CE methods for the analysis of phenols from olive oil use an aqueous electrolyte separation medium, although the importance of NACE is obvious, as this kind of CE seems to be more compatible with the hydrophobic olive oil matrix and could facilitate its direct injection. In the current work we develop a method involving SPE and NACE coupled to ESI-TOF MS. All the CE and ESI-TOF MS parameters were optimized in order to maximize the number of phenolic compounds detected and the sensitivity in their determination. Electrophoretic separation was carried out using a CE buffer system consisting of 25 mM NH(4)OAc/AcH in methanol/ACN (1/1 v/v) at an apparent pH value of 5.0. We studied in depth the effect of the nature and concentration of different electrolytes dissolved in different organic solvents and other experimental and instrumental CE variables. The results were compared with those obtained by CZE (with aqueous buffers) coupled to ESI-TOF MS; both methods offered to the analyst the chance to study phenolic compounds of different families (such as phenolic alcohols, lignans, complex phenols, flavonoids, etc.) from virgin olive oil by injecting methanolic extracts with efficient and fast CE separations. In the case of NACE method, we also studied the direct injection of the investigated matrix introducing a plug of olive oil directly into the capillary.

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Effect of the extraction solvent polarity on the sesame seeds oil composition

Tir

Dutta

Badjah‐Hadj‐Ahmed

2012

Euro J Lipid Sci & Tech

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This study highlights the effect of solvent polarity on the yield (Y%) and properties of oil extracted from Algerian sesame seeds. Extractions were carried out under Soxhlet conditions with the following solvents: hexane (Hx), ethanol (Eth), acetone (Ac), dichloromethane (Di), isopropanol (Iso), hexane:isopropanol (Hx:Iso), and chloroform:methanol (Chf:Me). The sesame oil yield obtained using different solvents ranged from 28.86 to 52.83%. Fatty acids and sterols analyses were performed by GC on capillary column. γ‐Tocopherol was the major tocochromanol compound detected by HPLC‐fluorescence. Fourteen fatty acids were identified, with the predominance of oleic and linoleic acids. The main sterol in sesame oil was β‐sitosterol, followed by stigmasterol, campesterol, and Δ5‐avenasterol which were present in lower concentrations. High correlations were found between arachidic, gadoleic, behenic, and lignoceric acids concentrations; these results were explained by the metabolic biosynthesis pathway of the biologically active long‐chain PUFA by successive elongation and desaturation. Principal component analysis (PCA) of the data obtained from sesame oil composition enabled an easy comparison of the different extraction solvents, and correlated their properties with the most characteristic components of the extracted oils with a view to understand solvent–oil interaction, and to establish the effects of extracting solvent on such oil composition.Practical applications: This study showed that the choice of solvent depends largely on the desired fraction to be extracted. Sesame oil was better extracted with less‐polar solvents but membrane‐associated lipids are more polar and require polar solvents capable of breaking hydrogen bonds or electrostatic forces. Owing to the differences in solvent capacity, the fatty acids, sterols, and tocopherols extracted along with the oil vary, leading to differences in the quality of the extracted oil. The results obtained in this study could be applied in industrial extraction to encourage the use of alternative extraction solvents.

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Characterization of hydroxyaromatic compounds in vegetable oils by capillary electrophoresis with direct injection in an oil‐miscible KOH/propanol/methanol medium

Cited by 13 publications

References 56 publications

Development of a non-aqueous capillary electrophoresis method with UV–visible and fluorescence detection for phenolics compounds in olive oil

Development of a non-aqueous capillary electrophoresis method with UV–visible and fluorescence detection for phenolics compounds in olive oil

NACE‐ESI‐TOF MS to reveal phenolic compounds from olive oil: Introducing enriched olive oil directly inside capillary

Effect of the extraction solvent polarity on the sesame seeds oil composition

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