Autologous Transplantation of Adipose-Derived Stem Cells Enhances Skin Graft Survival and Wound Healing in Diabetic Rats

Zografou, Aggeliki; Papadopoulos, Othon; Tsigris, Christos; Kavantzas, Nikolaos; Michalopoulos, Efstathios; Chatzistamatiou, Theofanis; Papassavas, Andreas; Stavropoulou-Gioka, Catherine; Dontas, Ismene; Περρέα, Δέσποινα

doi:10.1097/sap.0b013e31826af01a

Cited by 105 publications

(62 citation statements)

References 37 publications

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“…PHBV scaffold maintained 39 the wound moisture and demonstrated enough mechanical properties to withstand wound contraction. 40 Also, exudate and inflammatory cell infiltration enhanced the degradation of the structure, and thus heal- 41 ing progression. After 28 days all the wounds were closed and the PHBV scaffold was completely 42 degraded.…”

mentioning

confidence: 98%

“…Overexpression of TGF-b1 results in excessive fibroblast 516 migration and myofibroblast differentiation, and therefore on the 517 deposition of a highly organized ECM over-accumulation and scar 518 formation[46,47]. On the other side, TGF-b3 inhibits scarring by519 promoting normal collagen organization[41,48]. For instance, the 520 fetal skin can heal without scars and this phenomenon is associ-521 ated with a decrease in TGF-b1 and TGF-b2 expression, accompa-522 nied by increased or prolonged TGF-b3 expression[48,49].Interestingly, the presence of PHBV scaffold reduced the gene 524 expression of TGF-b1 and the combination with ASCs also 525 increased TGF-b3 gene expression.…”

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confidence: 99%

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Polyhydroxybutyrate-co-hydroxyvalerate structures loaded with adipose stem cells promote skin healing with reduced scarring

et al. 2015

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confidence: 98%

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confidence: 99%

Polyhydroxybutyrate-co-hydroxyvalerate structures loaded with adipose stem cells promote skin healing with reduced scarring

et al. 2015

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“…Autologous ADSCs transplantation could enhance skin graft survival in diabetic rats through differentiation, vasculogenesis, and secretion of growth factors, such as VEGF and TGF-β3. This might represent a novel therapeutic approach in skin graft surgery for diabetic wounds [48] . MSCs combined with plastic surgery or skin graft therapy may be a promising therapeutic approach for improving radiation-induced skin and muscle damages [49] .…”

Section: Necrosismentioning

confidence: 99%

New advances in the mesenchymal stem cells therapy against skin flaps necrosis

Zhang¹

2014

WJSC

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Mesenchymal stem cells (MSCs), multipotential cells that reside within the bone marrow, can be induced to differentiate into various cells, such as osteoblasts, adipocytes, chondrocytes, vascular endothelial progenitor cells, and other cell types. MSCs are being widely studied as potential cell therapy agents due to their angiogenic properties, which have been well established by in vitro and in vivo researches. Within this context, MSCs therapy appears to hold substantial promise, particularly in the treatment of conditions involving skin grafts, pedicle flaps, as well as free flaps described in literatures. The purpose of this review is to report the new advances and mechanisms underlying MSCs therapy against skin flaps necrosis.

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“…Studies have been done in rats comparing qualitatively peritoneal and inguinal region tissues 19 Studies with models of grafts 26 , and flaps 27 using the inguinal region ADSC showed increased viability of the necrotic area, however the route of administration of ADSC was subcutaneously. In the present study, the route of administration was intravenous, finding similar results to those mentioned, which…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats

et al. 2014

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PURPOSE:To evaluate the viability of random pattern dorsal skin flaps in rats after injection of adipose-derived stem cells (ADSC). METHODS:Thirty five adult male Wistar EPM rats (weight 250-300 g) were distributed, at random, in two groups. I-Control (flap elevation with injection of saline solution) with fifteen animals and II-Experimental (flap elevation with injection of ADSC ) with fifteen animal. The ADSC were isolated from others five adult male rats. A dorsal skin flap measuring 10x4 cm was raised and a plastic barrier was placed between the flap and its bed in both groups and the injection (cells or saline solution) were perfomed immediately after the surgery. The percentage of flap necrosis was measured on the seventh postoperative day. RESULTS:The ADSC were able to replicate in our culture conditions. We also induced their adipogenic, osteogenic and chondrogenic differentiation to verify their mesenchymal stem cells potentiality in vitro. The results were statistically significant showing that the ADSC decreased the area of necrosis (p<0.05). CONCLUSIONS:The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro. The administration of adipose-derived stem cells was effective to increase the viability of the random random pattern dorsal skin flaps in rats.

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Autologous Transplantation of Adipose-Derived Stem Cells Enhances Skin Graft Survival and Wound Healing in Diabetic Rats

Cited by 105 publications

References 37 publications

Polyhydroxybutyrate-co-hydroxyvalerate structures loaded with adipose stem cells promote skin healing with reduced scarring

Polyhydroxybutyrate-co-hydroxyvalerate structures loaded with adipose stem cells promote skin healing with reduced scarring

New advances in the mesenchymal stem cells therapy against skin flaps necrosis

Adipose-derived stem cells (ADSC) in the viability of a random pattern dorsal skin flap in rats

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