Autologous platelet-rich fibrin stimulates canine periodontal regeneration

Abstract

Platelet-rich fibrin (PRF) provides a scaffold for cell migration and growth factors for promoting wound healing and tissue regeneration. Here, we report using PRF in periodontal healing after open flap debridement (OFD) in canine periodontitis. A split-mouth design was performed in twenty dogs. Forty periodontitis surgical sites were randomly categorized into 2 groups; OFD alone and OFD with PRF treatment. Clinical parameters of periodontal pocket depth, gingival index, and the cemento-enamel junction-alveolar bone levels/root length ratio were improved in the OFD + PRF group. The OFD + pRf group also demonstrated a dramatically decreased inflammatory score compared with the OFD group. Collagen accumulation was improved in the OFD + PRF group at later time points compared with baseline. PRF application also significantly reduced inflammatory cytokine expression (TNFA and IL1B), and promoted the expression of collagen production-related genes (COL1A1, COL3A1, and TIMP1) and growth factors (PDGFB, TGFB1, and VEGFA). These findings suggest that PRF combined with OFD provides a new strategy to enhance the overall improvement of canine periodontitis treatment outcomes, especially in terms of inflammation and soft tissue healing. Therefore, PRF use in treating periodontitis could play an important role as a regenerative material to improve canine periodontitis treatment.

Effects of Autologous Platelet-Rich Fibrin in Post-Extraction Alveolar Sockets: A Randomized, Controlled Split-Mouth Trial in Dogs with Spontaneous Periodontal Disease

“…Some laboratory studies were carried out on platelets and leukocyte contents, and on the amount of GFs in both standard and advanced PRF in humans [11,12,[60][61][62]. Several studies have reported the amount of GFs in canine PRF; however, to the authors' knowledge, a complete characterization of the canine PRF has not yet been performed [46,52,63].…”

“…In some studies, the PRF has been tested on the healing of canine extraction sockets and in peri-implant sites, however all of these studies were experimental; therefore, lesions and tooth extractions were specially created in healthy dogs, which were used animal models for preclinical purposes [45][46][47][48][49][50][51][52][53].…”

Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro

“…Autologous platelet rich fibrin was chosen to improve periodontal regeneration 26 , 27 . The study in dogs demonstrated that the combination of autologous PRF with open flap debridement improved periodontal pocket depth and cemento-enamel-junction-alveolar bone levels/root length ratio 15 . In addition, the inflammatory score was decreased in open flap debridement with PRF application compared with the control 15 , implying the beneficial utilization of PRF in periodontal regenerative treatment.…”

“…The preparation of PRF is an easy and cost-effective way in clinical use in human study. Our previous report demonstrated the use of autologous PRF in periodontal regenerative treatment in dogs 15 . However, a high-volume collection of autologous blood in small dog breeds has technical difficulty.…”

Biomimetic Aspects of Oral and Dentofacial Regeneration

“…In patients with chronic periodontitis, the use of 1% metformin and PRF showed more pocket depth reduction and relative attachment level than either group alone [ 283 ]. Along with open-flap debridement, PRF has been shown to increase canine periodontitis treatment outcomes in animal studies [ 284 ].…”