Autologous keratinocytes cultured on benzylester hyaluronic acid membranes in the treatment of chronic full-thickness ulcers

Hollander, Dirk A.; Stein, Monika; Bernd, August; Windolf, Joachim; Pannike, A.

doi:10.12968/jowc.1999.8.7.25893

Cited by 25 publications

(10 citation statements)

References 34 publications

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“…To our knowledge, this stem cell source is previously unreported, representing a promising cell source for activating scaffolds for tissue regeneration. Previous results showed that autologous cells were more effective than heterologous cells in scaffold bioactivation for dermal regeneration (Hollander et al, 1999;Wisser and Steffes, 2003). Here we show that SGSCs are easy to isolate, giving rise to an adherent cell population that can be robustly expanded ex vivo (Figure 1b), allowing one to obtain therapeutic numbers of autologous cells in clinical time frames.…”

Section: Discussionsupporting

confidence: 50%

The Use of Human Sweat Gland–Derived Stem Cells for Enhancing Vascularization during Dermal Regeneration

Danner

Kremer

Petschnik

et al. 2012

Journal of Investigative Dermatology

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Vascularization is a key process in tissue engineering and regeneration and represents one of the most important issues in the field of regenerative medicine. Thus, several strategies to improve vascularization are currently under clinical evaluation. In this study, stem cells derived from human sweat glands were isolated, characterized, seeded in collagen scaffolds, and engrafted in a mouse full skin defect model for dermal regeneration. Results showed that these cells exhibit high proliferation rates and express stem cell and differentiation markers. Moreover, cells responded to angiogenic environments by increasing their migration (P<0.001) and proliferation (P<0.05) capacity and forming capillary-like structures. After seeding in the scaffolds, cells distributed homogeneously, interacting directly with the scaffold, and released bioactive molecules involved in angiogenesis, immune response, and tissue remodeling. In vivo, scaffolds containing cells were used to induce dermal regeneration. Here we have found that the presence of the cells significantly improved vascularization (P<0.001). As autologous sweat gland-derived stem cells are easy to obtain, exhibit a good proliferation capacity, and improve vascularization during dermal regeneration, we suggest that the combined use of sweat gland-derived stem cells and scaffolds for dermal regeneration might improve dermal regeneration in future clinical settings.

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Section: Discussionsupporting

confidence: 50%

The Use of Human Sweat Gland–Derived Stem Cells for Enhancing Vascularization during Dermal Regeneration

Danner

Kremer

Petschnik

et al. 2012

Journal of Investigative Dermatology

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“…Recently, research efforts have shifted from multilayered differentiated cultured skin equivalents toward transplantation of: (1) single‐cell suspension of autologous cultured keratinocytes 28–32 or combined cultured keratinocytes and fibroblasts, 33 or (2) monolayers of autologous keratinocytes cultured on biodegradable 34,35 or synthetic membranes 36 …”

mentioning

confidence: 99%

Autologous cultured keratinocytes on porcine gelatin microbeads effectively heal chronic venous leg ulcers

Liu

Hafner

Dragieva

et al. 2004

Wound Repair Regeneration

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We have established a specific bioreactor microcarrier cell culture system using porcine gelatin microbeads as carriers to produce autologous keratinocytes on a large scale. Moreover, we have shown that autologous keratinocytes can be cultured on porcine collagen pads, thereby forming a single cell layer. The objective of this study was to compare efficacy and safety of autologous cultured keratinocytes on microbeads and collagen pads in the treatment of chronic wounds. Fifteen patients with recalcitrant venous leg ulcers were assigned to three groups in a single-center, prospective, uncontrolled study: five underwent a single treatment with keratinocyte monolayers on collagen pads (group 1); another five received a single grafting with keratinocyte-microbeads (group 2); and the last five received multiple, consecutive applications of keratinocyte-microbeads 3 days apart (group 3). All patients were followed for up to 12 weeks. By 12 weeks, there was a mean reduction in the initial wound area of 50, 83, and 97 percent in the three groups, respectively. The changes in wound size were statistically significant between the first and third groups (p= 0.0003). Keratinocyte-microbeads proved to be more effective than keratinocyte monolayers on collagen pads when the former were applied every 3 days. Rapid availability within 10-13 days after skin biopsy and easy handling represent particular advantages.

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“…With the exception of hyaluronan, all of these (heparan sulfate, chondroitin sulfate, keratan sulfate, and dermatan sulfate) are sulfated branch-chained molecules that are conjugated to proteins in the form of proteoglycans. Scaffolds based on chondroitin sulfate (145,146) and hyaluronan (147,148) have been used for growing keratinocyte autografts. Besides purified ECM molecules, heterogenous mixtures of ECM components can also be used to promote keratinocyte differentiation in vitro.…”

Section: Naturally Occurring and Artificially Synthesized Extracellulmentioning

confidence: 99%

Directing stem cells into the keratinocyte lineage in vitro

Heng

Cao

Liu

et al. 2005

Experimental Dermatology

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A major area of research in regenerative medicine is the potential application of stem cells in skin grafting and tissue engineering. This would require well defined and efficient protocols for directing the commitment and differentiation of stem cells into the keratinocyte lineage, together with their selective purification and proliferation in vitro. The development of such protocols would reduce the likelihood of spontaneous differentiation of stem cells into divergent lineages upon transplantation, as well as reduce the risk of teratoma formation in the case of embryonic stem cells. Additionally, such protocols could provide useful in vitro models for studying skin tissue biology, as well as facilitate the genetic manipulation of stem cells for therapeutic applications. The development of pharmacokinetic and cytotoxicity/genotoxicity screening tests for skin-related biomaterials and drugs could also utilize protocols developed for the commitment and differentiation of stem cells into the keratinocyte lineage. Hence, this review critically examines the various strategies that could be employed to direct the commitment and differentiation of stem cells into the keratinocyte lineage in vitro.

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Autologous keratinocytes cultured on benzylester hyaluronic acid membranes in the treatment of chronic full-thickness ulcers

Cited by 25 publications

References 34 publications

The Use of Human Sweat Gland–Derived Stem Cells for Enhancing Vascularization during Dermal Regeneration

The Use of Human Sweat Gland–Derived Stem Cells for Enhancing Vascularization during Dermal Regeneration

Autologous cultured keratinocytes on porcine gelatin microbeads effectively heal chronic venous leg ulcers

Directing stem cells into the keratinocyte lineage in vitro

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