Autologous Epidermal Cultures and Narrow-Band Ultraviolet B in the Surgical Treatment of Vitiligo

Pianigiani, Elisa; Risulo, Massimiliano; Andreassi, A.; Taddeucci, Paolo; Ierardi, Francesca; Andreassi, Lucio

doi:10.1111/j.1524-4725.2005.31036

Cited by 23 publications

(20 citation statements)

References 21 publications

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“…From a small donor skin sample, an epidermal suspension is made by 0.25% trypsin digestion and seeded in culture flasks; epidermal sheets are harvested 3 weeks later from the culture vessel and transplanted onto depigmented recipient sites previously denuded with liquid nitrogen freezing (Falabella et al., 1989), superficial dermabrasion, CO 2 lasers, pulsed Erbium‐YAG lasers (Kaufmann et al., 1998) (Guerra et al., 2003) or diathermo‐surgery (Guerra et al., 2000). A hyaluronic artificial matrix for growing keratinocytes and melanocytes, has also been used with success (Andreassi et al., 1998; Pianigiani et al., 2005).…”

Section: Surgical Methodsmentioning

confidence: 99%

Update on skin repigmentation therapies in vitiligo

Falabella

Barona

2009

Pigment Cell Melanoma Res

136

121

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SummaryTreatment for vitiligo is difficult and prolonged. Nevertheless, at present considerable knowledge accumulated during several decades on the pathogenic mechanisms, revealed important clues for designing new strategies to improve vitiligo depigmentation. With available medical therapies, high repigmentation percentages mostly on facial and neck lesions are achieved, although they are less effective on trunk and limbs and poor on the acral parts of the extremities. Narrow band UVB and psoralens and UVA are the two most important treatments for generalized vitiligo affecting more than 10-20% of the cutaneous surface, and topical corticosteroids, or calcineurin inhibitors are the most valuable treatments for localized vitiligo. Persistence of achieved regimentation is variable and an undefined percentage of patients may have variable recurrence.When vitiligo becomes refractory, surgical methods may improve depigmentation as effectively as with medical therapy; in segmental (unilateral) or long standing, non-segmental (bilateral) stable vitiligo, repigmentation with surgical methods is usually permanent.

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Section: Surgical Methodsmentioning

confidence: 99%

Update on skin repigmentation therapies in vitiligo

Falabella

Barona

2009

Pigment Cell Melanoma Res

136

121

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“…52 Surgical techniques-restricted to stable vitiligo and used only when medical therapies have failed-have shown promising results, especially when followed by UVB or UVA exposure. [53][54][55] A. Considerations About the Use of Vitamin D Analogs in Vitiligo Therapy…”

Section: Therapeutic Approaches In Vitiligomentioning

confidence: 99%

New insights on therapy with vitamin D analogs targeting the intracellular pathways that control repigmentation in human vitiligo

Birlea¹,

Costin

Norris³

2009

Medicinal Research Reviews

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Vitiligo is a progressive depigmenting disorder affecting 0.5-2% of the population worldwide in which destruction of functional melanocytes from epidermis is a composite of multiple processes influencing melanocyte function and survival. A number of studies have recently reported that the treatment with vitamin D compounds or their combination with ultraviolet light or corticosteroids enhances repigmentation in vitiligo; however, the causal relationship at the cellular and molecular levels has not so far been investigated. This review details the main intracellular pathways through which vitamin D ligands alone or in different combinations may contribute to pigment restoration in vitiligo, outlines the most recent achievements in vitiligo treatment using vitamin D analogs, and compares their efficacy with other treatment regimens in vitiligo. Based on the reviewed literature and data, we foresee the need for designing new vitamin D compounds to achieve maximal therapeutic efficacy. Such compounds should have high selectivity for melanocytes, stimulatory effects on melanogenesis, and minimal suppressive effects on melanocyte growth. An approach combining clinical investigations and focused research on the cellular and molecular mechanisms of vitiligo repigmentation will lead to finding better treatments for this disease.

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“…[3–8] The major advantage of non-cultured cell suspension and culture-expanded cell suspension is that they permit the treatment of the affected area many folds larger than the donor area. [9–11] However, culture techniques employed for the expansion of the cell number are time consuming, expensive, and also require trained manpower and well-equipped tissue culture laboratory. In addition, there are concerns about the safety of the techniques owing to the xenobiotic properties of some of the additives in the culture medium.…”

Section: Introductionmentioning

confidence: 99%

Extracted hair follicle outer root sheath cell suspension for pigment cell restoration in vitiligo

Kumar

Mohanty

Sahni

et al. 2013

J Cutan Aesthet Surg

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Vitiligo surgery has come up a long way from punch skin grafts to epidermal cell suspension and latest to the extracted hair follicle outer root sheath cell suspension (EHF-ORS-CS) transplantation. The progressive development from one technique to the other is always in a quest for the best. In the latest development- EHF-ORS-CS, which is an enriched source of follicular inactive melanocyte (melanocyte stem cells), seems to be a good addition to the prevailing cell-based therapies for vitiligo; however, need to be explored further in larger, and preferably randomized blinded studies. This review discusses the principle, technical details, and stem cell composition of hair follicular outer root sheath cell suspension.

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Autologous Epidermal Cultures and Narrow-Band Ultraviolet B in the Surgical Treatment of Vitiligo

Abstract: This therapy can be considered for the treatment of stable vitiligo (especially focal and segmental) resistant to standard therapies. Their results are encouraging from the clinical and esthetic point of view, although the treatment is costly and highly specialized.

Cited by 23 publications

References 21 publications

Update on skin repigmentation therapies in vitiligo

Update on skin repigmentation therapies in vitiligo

New insights on therapy with vitamin D analogs targeting the intracellular pathways that control repigmentation in human vitiligo

Extracted hair follicle outer root sheath cell suspension for pigment cell restoration in vitiligo

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