Extracted hair follicle outer root sheath cell suspension for pigment cell restoration in vitiligo

Kumar, Anil; Mohanty, Sujata; Sahni, Kanika; Kumar, Rajesh; Gupta, Somesh

doi:10.4103/0974-2077.112679

Cited by 25 publications

(15 citation statements)

References 49 publications

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“…The results of the follicular unit ORS suspension showed stable repigmentation in over 75% patients at 1 year. [ 61 ] Similar results are reported with ORS-derived melanocyte (plucked hair) suspension technique as well. [ 62 ] The technique, though simple, is skill-dependent to harvest in FUE technique.…”

Section: Melanocyte Enriched Cellular Grafting Techniques In Vitiligosupporting

confidence: 79%

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Novel expansion techniques for skin grafts

Kadam

2016

Indian J Plast Surg

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The quest for skin expansion is not restricted to cover a large area alone, but to produce acceptable uniform surfaces, robust engraftment to withstand mechanical shear and infection, with a minimal donor morbidity. Ease of the technique, shorter healing period and reproducible results are essential parameters to adopt novel techniques. Significant advances seen in four fronts of autologous grafting are: (1) Dermal–epidermal graft expansion techniques, (2) epidermal graft harvests technique, (3) melanocyte-rich basal cell therapy for vitiligo and (4) robust and faster autologous cell cultures. Meek's original concept that the sum of perimeter of smaller grafts is larger than the harvested graft, and smaller the graft size, the greater is the potential for regeneration is witnessed in newer modification. Further, as graft size becomes smaller or minced, these micrografts can survive on the wound bed exudate irrespective of their dermal orientation. Expansion produced by 4 mm × 4 mm sized Meek micrografts is 10-folds, similarly 0.8 mm × 0.8 mm size micrografts produce 100-fold expansion, which becomes 700-fold with pixel grafts of 0.3 mm × 0.3 mm size. Fractional skin harvest is another new technique with 700 μ size full thickness graft. These provide instant autologous non-cultured graft to cover extensive areas with similar quality of engraftment surface as split skin grafts. Newer tools for epidermal blister graft harvest quickly, with uniform size to produce 7-fold expansions with reproducible results. In addition, donor area heals faster with minimal scar. Melanocyte-rich cell suspension is utilised in vitiligo surgery tapping the potential of hair root melanocytes. Further advances in the cell culture to reduce the cultivation time and provide stronger epidermal sheets with dermal carrier are seen in trials.

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Section: Melanocyte Enriched Cellular Grafting Techniques In Vitiligosupporting

confidence: 79%

“…The adaptation FUE technique is based on the evidence that they contain more potential melanocytic ‘stem cells’ than plucked hair follicle and its successful application in hair transplantation in contrast to the plucked hair transplants. [ 61 ]…”

Section: Melanocyte Enriched Cellular Grafting Techniques In Vitiligomentioning

confidence: 99%

Novel expansion techniques for skin grafts

Kadam

2016

Indian J Plast Surg

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“…[6] Surgical treatment is indicated in stable disease not responding to medical treatment. Surgical modalities are divided into tissue grafts and cellular grafts.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of efficacy of epidermal melanocyte transfer versus hair follicular melanocyte transfer in stable vitiligo

Donaparthi

Chopra

2016

Indian J Dermatol

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Background:Vitiligo surgery has come up a long way from punch skin grafts to epidermal cell suspension and latest to the extracted hair follicle outer root sheath cell suspension (EHFORSCS) transplantation. The progressive development from one technique to the other is always on a quest for the best. In the latest development, EHFORSCS, which is an enriched source of follicular inactive melanocyte (melanocyte stem cells), seems to be a good addition to the prevailing cell-based therapies for vitiligo. However, it needs to be explored further in larger, clinical trials.Methodology:A total of 11 patients with sixty stable vitiligo sites attending dermatology outpatient department were included for the open-labeled, prospective, comparative study. The sites were sequentially distributed into two groups of thirty each. Sites of one group were subjected to epidermal melanocyte transfer (EMT) and the others to hair follicular melanocyte transfer (HFMT). Response to treatment was evaluated on the basis of degree of repigmentation; final evaluation of area of involvement was done after completion of 6 months.Results:At the end of 6 months, repigmentation >90% was observed in 83.33% patches of EMT group and 43.33% in HFMT group. Repigmentation >75% was observed in 90% of patches in Group A and 43.34% of patches in Group B, respectively. There was statistically significant difference in the overall pigmentation between these two groups.Conclusion:Both noncultured autologous epidermal cell suspension transfer and noncultured EHFORSCS transfer are safe and effective surgical modalities in the management of stable vitiligo though EMT has shown a better response in the present study. Outer root sheath cell suspension transfer is a novel, minimally invasive technique in its nascent stage in the surgical management of vitiligo which requires further larger clinical trials for evaluation of its efficacy.

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“…The skin stem cells (SSCs) are in clinical set up for a long period of time and many cell based applications are there for the management of vitiligo, burn and other pigmentary disorders. Some investigators have used hair follicle stem cells (HFSC) for cell based clinical needs, especially in vitiligo 14 15 16 . We under-took this preliminary study to evaluate the HFSCs in comparison to SSCs to establish a candidate cell for the differentiation into melanocyte and neuronal lineage.…”

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confidence: 99%

Hair & skin derived progenitor cells: In search of a candidate cell for regenerative medicine

et al. 2016

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Background & objectives:Skin is an established tissue source for cell based therapy. The hair follicle has been introduced later as a tissue source for cell based therapy. The ease of tissue harvest and multipotent nature of the resident stem cells in skin and hair follicle has promoted basic and clinical research in this area. This study was conducted to evaluate skin stem cells (SSCs) and hair follicle stem cells (HFSCs) as candidate cells appropriate for neuronal and melanocyte lineage differentiation.Methods:In this study, SSCs and hair follicle stem cells (HFSCs) were expanded in vitro by explant culture method and were compared in terms of proliferative potential and stemness; differentiation potential into melanocytes and neuronal lineage.Results:SSCs were found to be more proliferative in comparison to HFSCs, however, telomerase activity was more in HFSCs in comparison to SSCs. Capacity to differentiate into two lineages of ectoderm origin (neuronal and melanocyte) was found to be different. HFSCs cells showed more propensities towards melanocyte lineage, whereas SSCs were more inclined towards neuronal lineage.Interpretation & conclusions:The study showed that SSCs had differential advantage over the HFSCs for neuronal cell differentiation, whereas, the HFSCs were better source for melanocytic differentiation.

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Extracted hair follicle outer root sheath cell suspension for pigment cell restoration in vitiligo

Cited by 25 publications

References 49 publications

Novel expansion techniques for skin grafts

Novel expansion techniques for skin grafts

Comparative study of efficacy of epidermal melanocyte transfer versus hair follicular melanocyte transfer in stable vitiligo

Hair & skin derived progenitor cells: In search of a candidate cell for regenerative medicine

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