Autologous derived, platelet-rich plasma gel in the treatment of nonhealing diabetic foot ulcer: a case report

Slesaczeck, Torsten; Paetzold, Hartmut; Nanning, Thomas; Reichel, Andreas; Barthel, Andreas; Bornstein, Stefan R.; Weck, M

doi:10.1177/2042018812438213

Cited by 12 publications

(8 citation statements)

References 9 publications

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“…Human derived autologous platelet-rich plasma (PRP) is also a promising wound healing treatment, due to the mitogenic and chemoattracting effects exerted by growth factors released at high concentrations after platelet α -granules degranulation [ 12 ]. Some case reports and few clinical trials [ 13 – 15 ] showed positive effects of PRP on reepithelialization of nonhealing wounds.…”

Section: Introductionmentioning

confidence: 99%

Hepatocyte Growth Factor Effects on Mesenchymal Stem Cells Derived from Human Arteries: A Novel Strategy to Accelerate Vascular Ulcer Wound Healing

Valente

Ciavarella

Pasanisi

et al. 2016

Stem Cells International

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Vascular ulcers are a serious complication of peripheral vascular disease, especially in diabetics. Several approaches to treat the wounds are proposed but they show poor outcomes and require long healing times. Hepatocyte Growth Factor/Scatter Factor (HGF/SF) is a pleiotropic cytokine exerting many biological activities through the c-Met receptor. This study was aimed at verifying whether HGF/SF influences proliferation, migration, and angiogenesis on mesenchymal stem cells isolated from human arteries (hVW-MSCs). hVW-MSCs were exposed to NIBSC HGF/SF (2.5, 5, 10, and 70 ng/mL) from 6 hrs to 7 days. HGF and c-MET mRNA and protein expression, cell proliferation (Alamar Blue and Ki–67 assay), migration (scratch and transwell assays), and angiogenesis (Matrigel) were investigated. hVW-MSCs displayed stemness features and expressed HGF and c-MET. HGF/SF did not increase hVW-MSC proliferation, whereas it enhanced the cell migration, the formation of capillary-like structures, and the expression of angiogenic markers (vWF, CD31, and KDR). The HGF/SF effects on hVW-MSC migration and angiogenic potential are of great interest to accelerate wound healing process. Local delivery of HGF/SF could therefore improve the healing of unresponsive vascular ulcers.

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Section: Introductionmentioning

confidence: 99%

Hepatocyte Growth Factor Effects on Mesenchymal Stem Cells Derived from Human Arteries: A Novel Strategy to Accelerate Vascular Ulcer Wound Healing

Valente

Ciavarella

Pasanisi

et al. 2016

Stem Cells International

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“…PRPT is reserved as a second-line therapy similar to HBO, especially in the treatment of refractory wounds [63, 102]. …”

Section: Discussionmentioning

confidence: 99%

“…After that time, the healing function is taken over by macrophages. Experimental and clinical studies demonstrated the most profound accelerating effect on wound healing in the 3rd week after PRP application [55, 102]. …”

Section: Autologous Platelet-rich Plasma Therapy (Prpt)mentioning

confidence: 99%

“…Preliminary results of basic studies and preclinical and clinical trials have not been confirmed in large controlled studies. Available analyses in case reports [62, 67], case series [59, 64], randomised controlled studies [57, 60], cohort studies [58], and metaanalyses [65, 66] are based on small samples. However, they indicate that PRPT applied for nonhealing DFU is a more effective method compared to local conventional therapy.…”

Section: Autologous Platelet-rich Plasma Therapy (Prpt)mentioning

confidence: 99%

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Adjunct Methods of the Standard Diabetic Foot Ulceration Therapy

Waniczek

Kozowicz

Muc-Wierzgoń

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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The outcome of management of diabetic foot ulceration (DFU) is poor and insufficient. DFU therapy includes the standard management as debridement of the wound, revascularization procedures, off-loading of the ulcer and antibacterial actions, and supplementation of growth factors and cytokines, leading to stimulation of granulation, epidermization, and angiogenesis. The aim of the present review is to summarize the adjunct methods of the standard DFU therapy as hyperbaric oxygen therapy (HBOT), maggot therapy (MT), and platelet-rich plasma therapy (PRPT). The results of preclinical and clinical trials indicated that the methods may reduce time of therapy, short-term morbidity, and the risk of major amputation.

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“…PRP has been investigated by a number of disciplines including oral and maxillofacial reconstruction [52], cardiology [54], podiatry [55], and ophthalmology in addition to orthopedics [56,57]. Anitua et al [58] performed an in vitro analysis of cytokines and growth factor production in the presence of platelet-rich and platelet-poor preparations on tendon healing and found a significant difference favoring the platelet-rich clot.…”

Section: Platelet Rich Plasmamentioning

confidence: 99%

Augmentation techniques for isolated meniscal tears

Taylor

Rodeo

2013

Curr Rev Musculoskelet Med

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Meniscal tears are relatively common injuries sustained by athletes and non-athletes alike and have far reaching functional and financial implications. Studies have clearly demonstrated the important biomechanical role played by the meniscus. Long-term follow-up studies of post-menisectomy patients show a predisposition toward the development of degenerative arthritic changes. As such, substantial efforts have been made by researchers and clinicians to understand the cellular and molecular basis of meniscal healing. Proinflammatory cytokines have been shown to have a catabolic effect on meniscal healing. In vitro and some limited in vivo studies have shown a proliferative and anabolic response to various growth factors. Surgical techniques that have been developed to stimulate a healing response include mechanical abrasion, fibrin clot application, growth factor application, and attempts at meniscal neovascularization. This article discusses various augmentation techniques for meniscal repair and reviews the current literature with regard to fibrin clot, platelet rich plasma, proinflammatory cytokines, and application of growth factors.

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Autologous derived, platelet-rich plasma gel in the treatment of nonhealing diabetic foot ulcer: a case report

Cited by 12 publications

References 9 publications

Hepatocyte Growth Factor Effects on Mesenchymal Stem Cells Derived from Human Arteries: A Novel Strategy to Accelerate Vascular Ulcer Wound Healing

Hepatocyte Growth Factor Effects on Mesenchymal Stem Cells Derived from Human Arteries: A Novel Strategy to Accelerate Vascular Ulcer Wound Healing

Adjunct Methods of the Standard Diabetic Foot Ulceration Therapy

Augmentation techniques for isolated meniscal tears

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