Autoinducer 2 of Fusobacterium nucleatum as a target molecule to inhibit biofilm formation of periodontopathogens

Jang, Young Eun; Choi, Yu-Jung; Lee, Sung-Hoon; Jun, Hye‐Kyoung; Choi, Bong‐Kyu

doi:10.1016/j.archoralbio.2012.04.016

Cited by 67 publications

(69 citation statements)

References 40 publications

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“…Several reports describe the inhibition of biofilm formation by S. mutans through the interference with the AI-2 QS system reducing significantly the biomass, the average depth, and the maximum depth of the biofilm [18]. However, the structure of the AI-2 produced by streptococci is unknown [22], more studies being needed to understand the interference of 20J extracts and other inhibitors with the AI-2 QS system in this species.…”

Section: Discussionmentioning

confidence: 99%

“…The mutation of the gene of luxS which codifies the enzyme required for AI-2 production affects biofilm formation in S. mutans [12,17]. The QS signal AI-2 seems to be relevant also in other oral pathogens such as Porphyromonas gingivalis, Aggregatibacteractinomycetencomitans Y4, Treponema denticola , and Tannerella forsythia [7,18], which indicates complex signalling networks in the oral biofilm. According to the hypothesis proposed by Kolenbrander et al [19], high levels of AI-2 would accelerate the growth of pathogens while reducing growth of commensal bacteria, contributing to subgingival plaque formation and maturation and leading to periodontal diseases.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition ofSteptococcus mutansbiofilm formation by extracts ofTenacibaculumsp. 20J, a bacterium with wide-spectrum quorum quenching activity

Muras

Mayer

Romero

et al. 2018

Journal of Oral Microbiology

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Background: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent the biofilm formation by Streptococcus mutans, a pathogen involved in tooth decay. Objective: To obtain inhibition of biofilm formation by S. mutans by extracts obtained from the marine bacterium Tenacibaculum sp. 20J interfering with the AI-2 quorum sensing system. Design: The AI-2 inhibitory activity was tested with the biosensors Vibrio harveyi BB170 and JMH597. S. mutans ATCC25175 biofilm formation was monitored using impedance real-time measurements with the xCELLigence system®, confocal laser microscopy, and the crystal violet quantification method. Results: The addition of the cell extract from Tenacibaculum sp. 20J reduced biofilm formation in S. mutans ATCC25175 by 40–50% compared to the control without significantly affecting growth. A decrease of almost 40% was also observed in S. oralis DSM20627 and S. dentisani 7747 biofilms. Conclusions: The ability of Tenacibaculum sp. 20J to interfere with AI-2 and inhibit biofilm formation in S. mutans was demonstrated. The results indicate that the inhibition of quorum sensing processes may constitute a suitable strategy for inhibiting dental plaque formation, although additional experiments using mixed biofilm models would be required.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition ofSteptococcus mutansbiofilm formation by extracts ofTenacibaculumsp. 20J, a bacterium with wide-spectrum quorum quenching activity

Muras

Mayer

Romero

et al. 2018

Journal of Oral Microbiology

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“…The luxS gene from A. actinomycetemcomitans is, for instance, able to complement a luxS mutation in P. gingivalis [18]. Furthermore, AI-2 produced by Fusobacterium nucleatum stimulates co-aggregation and the expression of adhesion molecules in P. gingivalis, Treponema denticola, and Tannerella forsythia [19].…”

Section: Luxs/ai-2 Regulated Inter-species Interactions Of P Gingivalismentioning

confidence: 98%

LuxS signaling in Porphyromonas gingivalis-host interactions

2015

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“…In addition to streptococcal AI-2, P. gingivlais can response to AI-2 signal secreted by A. actinomycetemcomitans (Fong et al, 2001). AI-2 secreted by F. nucleatum is able to elicit changes in the expression of the representative adhesins of the so-called “red-complex” ( P. gingivalis , T. denticola , and T. forsythia ), and thus, increase the colonization of these periodontopathogens in oral multispecies biofilms (Jang et al, 2013a). …”

Section: Microbial Intercellular Signalingmentioning

confidence: 99%

Intercellular communications in multispecies oral microbial communities

Guo

Shi

2014

Front. Microbiol.

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The oral cavity contains more than 700 microbial species that are engaged in extensive cell–cell interactions. These interactions contribute to the formation of highly structured multispecies communities, allow them to perform physiological functions, and induce synergistic pathogenesis. Co-adhesion between oral microbial species influences their colonization of oral cavity and effectuates, to a large extent, the temporal and spatial formation of highly organized polymicrobial community architecture. Individual species also compete and collaborate with other neighboring species through metabolic interactions, which not only modify the local microenvironment such as pH and the amount of oxygen, making it more suitable for the growth of other species, but also provide a metabolic framework for the participating microorganisms by maximizing their potential to extract energy from limited substrates. Direct physical contact of bacterial species with its neighboring co-habitants within microbial community could initiate signaling cascade and achieve modulation of gene expression in accordance with different species it is in contact with. In addition to communication through cell–cell contact, quorum sensing (QS) mediated by small signaling molecules such as competence-stimulating peptides (CSPs) and autoinducer-2 (AI-2), plays essential roles in bacterial physiology and ecology. This review will summarize the evidence that oral microbes participate in intercellular communications with co-inhabitants through cell contact-dependent physical interactions, metabolic interdependencies, as well as coordinative signaling systems to establish and maintain balanced microbial communities.

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Autoinducer 2 of Fusobacterium nucleatum as a target molecule to inhibit biofilm formation of periodontopathogens

Cited by 67 publications

References 40 publications

Inhibition ofSteptococcus mutansbiofilm formation by extracts ofTenacibaculumsp. 20J, a bacterium with wide-spectrum quorum quenching activity

Inhibition ofSteptococcus mutansbiofilm formation by extracts ofTenacibaculumsp. 20J, a bacterium with wide-spectrum quorum quenching activity

LuxS signaling in Porphyromonas gingivalis-host interactions

Intercellular communications in multispecies oral microbial communities

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