Autofluorescence imaging for improved visualization of joint structures during arthroscopic surgery

Nguyen, Duy Tan; Horssen, Pepijn van; Derriks, Hans; Giessen, Martijn van de; Leeuwen, Ton G. van

doi:10.1186/s40634-017-0094-4

Cited by 5 publications

(4 citation statements)

References 44 publications

(51 reference statements)

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“…Despite the improved penetration depth of NIR imaging 51 , it is possible that the accumulation of FA-HA probes on cartilage tissue cannot be detected non-invasively. In that case, a fluorescence arthroscope may be used to image the probe accumulation on cartialge tissue in situ, which has been documented recently 52 .…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic Acid-Based Optical Probe for the Diagnosis of Human Osteoarthritic Cartilage

Hakamivala

Huang

et al. 2018

Nanotheranostics

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Osteoarthritis is typically caused by cartilage injury, followed by localized inflammatory responses and tissue deterioration. Early treatment of osteoarthritis is often impossible due to the lack of diagnostic options. Recent studies have supported that different imaging probes can be used for arthritis detection in mice. However, none of these diagnostic tools have been tested on human articular cartilage. To fill this gap, an optical imaging probe was developed to target activated macrophages and the accumulation of imaging probes on tissue was used to assess the severity of human osteoarthritis.Methods: The probe was fabricated using hyaluronic acid (HA) particles conjugated with near-infrared dye and folic acid (FA). The ability of the FA-HA probes to detect activated macrophages and quantify cartilage injury was evaluated using a cell culture model in vitro and human osteoarthritic cartilage explants ex vivo.Results: Our cell study results supported that the FA-HA probes are cell compatible (up to 0.5mg/mL) and can detect activated macrophages in 30 minutes. Using human articular cartilage, we verified the existence of activated macrophages on osteoarthritic cartilage with highly up-regulated expression of folate receptors (~13 folds by comparison with healthy control). In addition, we found that FA-HA probes had higher binding amounts (~3 folds) to osteoarthritic tissue than healthy ones. Histological analyses confirmed that there was a strong linear relationship (R=0.933) between the fluorescent intensity of tissue-associated probe and the extent of folate receptors on osteoarthritic cartilage. Finally, the co-localization of the imaging probe, folate receptors and cartilage degeneration on the tissue sections indicated the extraordinary accuracy and efficiency of this osteoarthritis diagnostic probe.Conclusions: Our results support the probe as an effective diagnostic tool to detect the area and severity of osteoarthritic human articular cartilage.

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Section: Discussionmentioning

confidence: 99%

Hyaluronic Acid-Based Optical Probe for the Diagnosis of Human Osteoarthritic Cartilage

Hakamivala

Huang

et al. 2018

Nanotheranostics

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“… 157 Mass spectrometry imaging enables the precise measurement of molecular masses in joint tissues, aiding biomarker identification. 158 Fluorescence spectroscopy is another method for studying specific biomarkers within joint tissues, 159 contributing to our understanding of their distribution and composition. Despite their potential, these techniques are not widely employed in clinical practice for OA.…”

Section: Therapeutic Potential Of Spatial Imaging In Oa Researchmentioning

confidence: 99%

Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications

Fan,

Sun,

Young

et al. 2024

Bone Res

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Osteoarthritis (OA) is a debilitating degenerative disease affecting multiple joint tissues, including cartilage, bone, synovium, and adipose tissues. OA presents diverse clinical phenotypes and distinct molecular endotypes, including inflammatory, metabolic, mechanical, genetic, and synovial variants. Consequently, innovative technologies are needed to support the development of effective diagnostic and precision therapeutic approaches. Traditional analysis of bulk OA tissue extracts has limitations due to technical constraints, causing challenges in the differentiation between various physiological and pathological phenotypes in joint tissues. This issue has led to standardization difficulties and hindered the success of clinical trials. Gaining insights into the spatial variations of the cellular and molecular structures in OA tissues, encompassing DNA, RNA, metabolites, and proteins, as well as their chemical properties, elemental composition, and mechanical attributes, can contribute to a more comprehensive understanding of the disease subtypes. Spatially resolved biology enables biologists to investigate cells within the context of their tissue microenvironment, providing a more holistic view of cellular function. Recent advances in innovative spatial biology techniques now allow intact tissue sections to be examined using various -omics lenses, such as genomics, transcriptomics, proteomics, and metabolomics, with spatial data. This fusion of approaches provides researchers with critical insights into the molecular composition and functions of the cells and tissues at precise spatial coordinates. Furthermore, advanced imaging techniques, including high-resolution microscopy, hyperspectral imaging, and mass spectrometry imaging, enable the visualization and analysis of the spatial distribution of biomolecules, cells, and tissues. Linking these molecular imaging outputs to conventional tissue histology can facilitate a more comprehensive characterization of disease phenotypes. This review summarizes the recent advancements in the molecular imaging modalities and methodologies for in-depth spatial analysis. It explores their applications, challenges, and potential opportunities in the field of OA. Additionally, this review provides a perspective on the potential research directions for these contemporary approaches that can meet the requirements of clinical diagnoses and the establishment of therapeutic targets for OA.

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“…Nguyen et al used arthroscopic autofluorescence imaging, assessed with excitation wavelengths scanned from 280 to 480 nm in 10 nm steps, and emissions assessed from 300 to 900 nm to improve the visualisation of the anterior cruciate ligament during reconstructive surgery, with a focus on improving contrast between the femoral insertion site and background using bovine and human knees [56]. A mean Dice coefficient of 0.84 was obtained for agreement between visible light and autofluorescence.…”

Section: Bone and Joint Healthmentioning

confidence: 99%

Clinical applications of non‐invasive multi and hyperspectral imaging of cell and tissue autofluorescence beyond oncology

Campbell

Mahbub

Habibalahi

et al. 2023

Journal of Biophotonics

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Hyperspectral and multispectral imaging of cell and tissue autofluorescence employs fluorescence imaging, without exogenous fluorophores, across multiple excitation/emission combinations (spectral channels). This produces an image stack where each pixel (matched by location) contains unique information about the sample's spectral properties. Analysis of this data enables access to a rich, molecularly specific data set from a broad range of cell‐native fluorophores (autofluorophores) directly reflective of biochemical status, without use of fixation or stains. This non‐invasive, non‐destructive technology has great potential to spare the collection of biopsies from sensitive regions. As both staining and biopsy may be impossible, or undesirable, depending on the context, this technology great diagnostic potential for clinical decision making. The main research focus has been on the identification of neoplastic tissues. However, advances have been made in diverse applications—including ophthalmology, cardiovascular health, neurology, infection, assisted reproduction technology and organ transplantation.

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Autofluorescence imaging for improved visualization of joint structures during arthroscopic surgery

Cited by 5 publications

References 44 publications

Hyaluronic Acid-Based Optical Probe for the Diagnosis of Human Osteoarthritic Cartilage

Hyaluronic Acid-Based Optical Probe for the Diagnosis of Human Osteoarthritic Cartilage

Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications

Clinical applications of non‐invasive multi and hyperspectral imaging of cell and tissue autofluorescence beyond oncology

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