Autoantibodies in Pemphigus Vulgaris

Immunofluorescence Studies on Addison’s Disease

“…From our experience over the last ten years with indirect IF in several clinical conditions such as chronic thyroiditis, myasthenia gravis and pemphigus [11,12,13], we have learned to avoid using sera in low dilutions and especially undiluted serum. A few pilot experi ments performed at the beginning of this study with sera diluted 1:3 gave a high incidence of nonspecific staining, leading us once again to the use of higher dilutions, starting with 1:10.…”

Absorption Studies on Antigen(s) of the Esophageal Mucosa Reactive with Autoantibodies of Pemphigus

“…The indirect I.F. staining technics and tests for activity and specificity of the sera were carried out by standard methods as previously described (1,2,4). Briefly, tissues were quick frozen in liquid nitrogen, sectioned at 4 //m in a cryostat, treated (unfixed) with the patient's serum and with a fluorescein conjugate of goat or rabbit anti-human IgG prepared according to standard methods (5).…”

“…Thus far, only stratified squamous epithelium has been demon strated to contain the reactive antigen (2). This report describes our initial studies on the extraction of antigcn(s) from the mucosa of human, monkey, and bovine esophagus.…”

Autoimmune Skin Diseases since 1963

“…Results were first pub lished in 1964 [9]. Only 1 year later bullous pemphigoid followed suit, demonstrating a basement membrane pattern of in vivo and in vitro binding of immunoglobulins [10]. About the same time, in 1966 we find the first hint of an autoimmune process in dermatitis herpetiformis, by Rudi Cormane of Amsterdam [11], elaborated in detail in 1969 by Jan van der Meer [12], Deposition of C3 in dermatitis herpetiformis was described in 1972 [ 13].…”