Authentication of equine DNA from highly processed donkey-hide glue (Colla Corii Asini) using SINE element

Lv, Pin; Zhao, Ying-Jie; Qi, Fei; Zhou, Xiang-Shan; You, Jinhua; Qin, Yufeng; Zhang, Yuanxing

doi:10.38212/2224-6614.2252

Cited by 5 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is in agreement with a report that ceramide inhibits melanogenesis by activation of ERK and inhibition of ERK and Akt causes an increase in pigmentation in human melanocytes [12]. Similarly, imidazole derivative and thaginin A can inhibit tyrosinase and activate the ERK pathway [19, [24][25][26].…”

Section: Discussionsupporting

confidence: 92%

Mechanisms of Anti-Melanogenic and Proliferative Effects of Colla Corii Asini (E’jiao)

2021

Internal Med Res Open J

View full text Add to dashboard Cite

Natural compounds have been reported for the development of cosmetic skin care products but it remains a challenge to find safe and useful ones. Colla corii asini (E'jiao), or donkey-hide gelatin (DHG) prepared by concentrating stewed Equus asinus L. donkey hide, has been useful in traditional Chinese medicine. However, its anti-melanogenic and skin care effects have not been explored. In this study, the biological effect of DHG was tested on murine B16-F10 melanocytes and human HaCaT keratinocytes. The cell signal pathway mechanisms by DHG were also examined by western blot assay. The results showed that DHG was not cytotoxic to cells and had an anti-melanogenic effect by inhibiting tyrosinase activity and reducing melanin contents in B16-F10 melanocytes. It also showed an anti-oxidative effect of reducing ROS generation in H 2 O 2 -stressed HaCaT keratinocytes. DHG dose-dependently enhanced phosphorylation of ERK, but reduced p-JNK and p-p38 proteins in B16-F10 cells under the UV stress. This is consistent with the results of other anti-melanogenic agents. Therefore, this suggested DHG had a potential for skin whitening and skin care applications.

show abstract

Section: Discussionsupporting

confidence: 92%

Mechanisms of Anti-Melanogenic and Proliferative Effects of Colla Corii Asini (E’jiao)

2021

Internal Med Res Open J

View full text Add to dashboard Cite

show abstract

“…For animal crude drugs, polymerase chain reaction (PCR) methods using species-speci c primers have been applied. [2][3][4] Direct sequencing methods using universal primers can target cytochrome b, [5][6][7] cytochrome c oxidase I (COI), 8,9 and 12S 5,10 and 16S 8,11 ribosome RNA genes. The development of molecular techniques has enabled identi cation of the origin of samples containing a mix of standard and nonstandard drugs.…”

Section: Introductionmentioning

confidence: 99%

A method for determining the animals of origin in crude drugs using MinION, a compact next-generation sequencer

Nakanishi

Takada

Yoneyama

et al. 2022

Preprint

View full text Add to dashboard Cite

We evaluated whether MinION, an inexpensive, portable sequencer, can be applied for identifying the animals of origin in crude drugs. Standard and nonstandard crude drugs with different species of origin were examined. In addition, the standards mixed with nonstandard samples were used. As a target gene, cytochrome c oxidase I was amplified and sequenced. The Fast mode results had a slightly lower match ratio than High-accuracy mode, but the animals of origin were correctly determined by BLAST for all samples. For antler velvet derived from Rangifer tarandus, even the sequences were aligned based on Cervus elaphus, the animal of origin was determined correctly. Minor contents could be detected from mixtures of two animals, if the mixtures contained at least 19:1 mtDNA when the coverage allele-fraction threshold was 0.05. By contrast, in Fast mode, two sequences could not be separated due to the low accuracy of the base-calling in each read. For field work, the species of origin of crude drugs could be identified, by only simple DNA extraction and library preparation. Therefore, MinION appears to be convenient tool for identifying the animal of origin in crude drugs.

show abstract

“…A colla corii asini ou donkey-hide gelatin, é uma gelatina extraída da pele da espécie Equus asinus, também chamada de ejiao. O produto é utilizado há mais de 2.000 anos na medicina tradicional chinesa nas terapias anti anêmia e antihemorrágica, além de consumido como alimento nutritivo que promove a saúde e vitalidade (Lv et al, 2011;Wu et al, 2007).…”

Section: Introductionunclassified

Panorama mundial das tecnologias referentes ao uso do ejiao por meio de busca em base de patentes

Fonseca

Almeida

Mariz

et al. 2021

RSD

View full text Add to dashboard Cite

O colla corii asini ou donkey-hide gelatin ou ejiao é uma gelatina obtida do colágeno da pele de jumentos, utilizado há milhares de anos com diversas finalidades na medicina tradicional chinesa. A demanda pelo ejiao, misturado a ervas e outros ingredientes da medicina chinesa, aumentou devido a ascensão econômica da população chinesa em geral. As peles de jumentos oriundas de países como o Quirguistão, o Brasil e o México seguem para a China para a produção do Ejiao. Nessa cadeia extrativista a África é o território mais afetado, tanto no número de animais abatidos quanto no impacto social e econômico. Este trabalho é um levantamento prospectivo de patentes sobre inovações e descobertas do uso terapêutico do ejiao. A pesquisa foi realizada em três bases de patentes e a partir destas foram recuperados o total de 1837 documentos sobre tecnologias que fazem uso do ejiao. Analisando a trajetória temporal das publicações na área, observa-se que as invenções tiveram início na década de 90 e atingiram seu ápice no ano de 2015, contudo observou-se uma recaída nos anos seguintes, a suposição é de que o declínio tenha sido ocasionado pela redução populacional dos asininos na China e nos países fornecedores de outras regiões do mundo, além não existência da cadeia produtiva de pele de jumentos.

show abstract

Authentication of equine DNA from highly processed donkey-hide glue (Colla Corii Asini) using SINE element

Cited by 5 publications

References 0 publications

Mechanisms of Anti-Melanogenic and Proliferative Effects of Colla Corii Asini (E’jiao)

Mechanisms of Anti-Melanogenic and Proliferative Effects of Colla Corii Asini (E’jiao)

A method for determining the animals of origin in crude drugs using MinION, a compact next-generation sequencer

Panorama mundial das tecnologias referentes ao uso do ejiao por meio de busca em base de patentes

Contact Info

Product

Resources

About