Autentikasi Daging Ayam Segar Dari Kontaminasi Daging Babi Menggunakan Gen Cyt-B Dengan Analisis Duplex- Polymerase Chain Reaction

Abstract

Halal is one of important aspects in consumer protection. Meat and processed meat products are food that should be controlled strictly because those are prone to be adulterated by pork contamination. Therefore, it is necessary to provide detection technique which is accurate, fast and cheap. The objective of this research was to identify the presence of impurities of pork meat on raw chicken meat using gene Cyt-b with duplex-PCR analysis. This research used six samples of raw chicken meat and raw pork. Raw chicken meat was bought from supermarkets in the city of Surakarta and raw pork was obtained from pig slaughterhouse. The percentage of raw pork contamination on raw chicken meat was designed as much as 1, 5, 10, and 25%, respectively. The DNA genome was isolated according to DNA isolation protocol from Genomic DNA Mini Kit. In addition, duplex-PCR was performed based on protocol of KAPA2G Fast Multiplex PCR kit. The data was descriptively analyzed by directly looking the DNA bands on the gel documentation apparatus. The result showed that specific DNA bands for chicken and pig were completely appeared on 1.5% of agarose gels. Duplex-PCR detect contamination of pork on raw meat of chicken at all contamination levels. This research proved that the duplex-PCR detect the contamination of pork until the level of 1%.

Development of mitochondrial 12S rRNA gene for identification of dog and rat in beef using multiplex PCR

“…This analysis performed to ensure that oligonucleotide primers designed in this study and other components of PCR were working together and they could be used as a marker for identification of dog and rat in food products containing beef and bovine derivatives. The studies in food authentication using PCR technology were reported using duplex PCR mitochondrial Cytochrome b gene to detect pork and chicken contamination in raw meats and meatballs using published primers (Ni'mah et al, 2016;Hertanto et al, 2017;Novianty et al, 2017). The use of mitochondrial 12S rRNA gene as a marker for species identification had been successfully conducted using sequence analysis of processed meats samples.…”

“…Meats adulteration is prohibited due to violation of consumer rights and rule of food safety, therefore, the effort to identify meat adulteration accurately is needed (Mahajan et al, 2010). In addition, Hertanto et al (2017) reported that contamination undesired species in meat products do not only happen intentionally but also accidentally. By using same meat grinder for different species without strict control will automatically contaminate meat.…”

“…Mitochondrial genome was well studied and widely used for species identification due to uniqueness of its nucleotide sequence. Previous studies successfully identified various species in the meats with and without temperature treatments by using multiplex PCR mitochondrial DNA cytochrome b gene (Matsunaga et al, 1999;Shin et al, 2006;Ni'mah et al, 2016;Hertanto et al, 2017). Irine et al (2013) also used cytochrome b gene to identify species of tiger, cat, and dog.…”

UJI COBA BEBERAPA KOMBINASI KOMPOSISI PAKAN TRENGGILING (Manis javanica Desmarest, 1822) DI PENANGKARAN

“…Keanekaragaman sifat dan ciri yang dimiliki trenggiling (Manis javanica Desmarest, 1822), menggambarkan potensi dan manfaat, diantara-nya adalah daging dan sisiknya yang diyakini sebagai obat dan makanan (Zainuddin, 2008;Hertanto, 2010). Menyadari akan keanekaragaman potensi, manfaat, dan nilai ekonomi yang dimiliki trenggiling, maka perlu dilakukan penelitian di penangkaran untuk memperoleh pengetahuan dan teknologi yang mendukung pelestarian serta pemanfaatannya secara tepat.…”