Auswirkungen der Leukozytendepletion auf die Lagerungsqualität von Vollblut

Neumeister, Birgid; Grauer, M; Koch, Matthias; Hörnlein, Rainer F; Dinkelmann, S.; Bernhard, R. D.; Haap, Michael; Northoff, Hinnak

doi:10.1007/s001010050496

Cited by 5 publications

(6 citation statements)

References 10 publications

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“…The coagulation factors V and VIII decreased to about 20% of normal in group WB and were considerably better preserved in group ldWB. Similar or slightly higher concentrations were found in other studies after the same time of storage [14,30,31]. Fibrin monomers and prothrombin fragments F1-2 show that there is little evidence for activation of the (terminal) coagulation cascade in most units of groups WB and PRC/FFP; in some there is a moderate increase.…”

Section: Discussionsupporting

confidence: 70%

“…The attending anesthetist and orthopedic surgeons, however, were not aware of the major aim of the study (homologous transfusion rate) but were informed about the minor aim only (parameters of quality of the stored blood). Bias usually does not extend beyond the major aim of the study and therefore is not expected to influ- [30,31,47]. ATP content of the erythrocytes was similar in all groups and within the range previously described or higher [30,48,49].…”

Section: Discussionmentioning

confidence: 76%

“…Permissible shelf time was utilized to the extent feasible in clinical routine, surgery was carried out in the 5th, 6th and 7th week in groups WB, ldWB and PRC/FFP, respectively. For group ldWB the recommended storage time of 5 weeks was exceeded by 1 week since recent studies show that parameters indicating quality of red cells are well preserved at storage times up to 49 days [30,31]. The transfusion of autologous plasma for volume substitution -and not for replacement of coagulation factors -is not in ac- The quality parameters determined on days 35, 42 and 49 in the unit donated first (first 10 patients in each group) are shown in table 3.…”

Section: Discussionmentioning

confidence: 99%

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The Type of Storage of Autologous Blood Does Not Influence Homologous Transfusion Requirements in Hip Arthroplasty

Lorentz¹,

Klever²,

Dempfle³

et al. 2001

Transfus Med Hemother

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Background: The new German regulations for hemotherapy require the use of autologous blood as buffy coat-poor packed red cells (PRC) and fresh frozen plasma (FFP) or as leukocytedepleted whole blood (ldWB). There are no data, however, documenting reduced allogeneic transfusion rates and/or a better outcome with PRC/FFP or ldWB. We investigated whether storage as processed blood results in a different rate of homologous transfusion in patients undergoing surgery for hip arthroplasty. Patients and Methods: After informed consent 94 patients (48 men, 46 women, age 36–78 years) scheduled for hip arthroplasty and predepositing autologous blood were allocated at random to either of three groups: group WB (predeposit stored as unprocessed whole blood, surgery in the 5th week after the first donation), group ldWB (leukocyte-depleted whole blood, surgery in week 6) and group PRC/FFP (packed red cells and fresh frozen plasma, surgery in week 7). Three units were requested, deferral hemoglobin concentration was 11 g/dl. Results: Hemoglobin concentration prior to donation was measured as 13.8 ± 1.0, 14.2 plusmn; 1.1, 14.0 plusmn; 1.1 g/dl in groups WB, ldWB and PRC/FFP, respectively (mean plusmn; standard deviation); hemoglobin concentration prior to surgery as 13.0 plusmn; 1.3*, 13.5 plusmn; 1.4, 13.8 plusmn; 1.1* g/dl (*p < 0,05). Blood loss amounted to 2,270 (970–3,760), 1,850 (930–3,680) and 1,680 (480–3,390) ml (median, range) (n.s.). Two patients in group WB, 2 in group ldWB, and 1 in group PRC/FFP required allogeneic transfusion (n.s.). Conclusions: The type of storage of autologous predeposit does not seem to influence the allogeneic transfusion rate in hip arthroplasty. The patients in groups ldWB and PRC/FFP were allowed more time for erythropoiesis, but red cells were lost due to processing of the blood. The data do not support the concept to leukocyte-deplete autologous blood or to produce components.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

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The Type of Storage of Autologous Blood Does Not Influence Homologous Transfusion Requirements in Hip Arthroplasty

Lorentz¹,

Klever²,

Dempfle³

et al. 2001

Transfus Med Hemother

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“…Auf die Konzentration des freien Hb und die Hämolyserate hatte die Leukozytendepletion in unserer Untersuchung keinen gravierenden Einfluû; unsere Werte für LKDPL-VB sind jedoch niedriger als die von Neumeister et al [21], was für den schonenden Umgang mit den Blutkonserven (-Proben) sprechen könnte. Laktat-und pH-Werte (Anstieg, Abfall) sind ebenfalls vergleichbar.…”

Section: Biochemieunclassified

Die Qualität von Vollblut in Abhängigkeit von Herstellungsverfahren (Leukozytendepletion) und Lagerungsdauer. Bedeutung für die operationsvorbereitende Eigenblutspende*

Bormann¹,

Wirtz²,

Weiler³

et al. 2000

Anasthesiol Intensivmed Notfallmed Schmerzthe

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Several parameters indicating quality of stored blood were constant in whole blood independent of the kind of preparation during a storage of 49 days. This is in contrast to the main part of specific scientific communications. A beneficial influence of leukocyte depletion was observed for some coagulation parameters whereas increasing characteristics of activated coagulation in CPDA1-stored whole blood at the end of storage time had to be observed. The preparation of whole CPDA1-blood is recommended for autologous predonation, if storage time does not exceed 30 days. Storage time of > 40 days seems to be possible for autologous whole blood after filtration for leucocyte depletion.

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“…Leucocyte-depleted whole blood was prepared using the in-line pre-storage filter system Leukotrap A1 (Pall, Muttenz, Switzerland) which included the WBF TM leucocyte removal filter. This system has previously been validated [21]and analysis of random specimens always showed <10 6 leucocytes/l after filtration. Pairs of specimens were taken prior to and following filtration and collected into polypropylene tubes (Sarstedt, Sevelen, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

et al. 2000

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Background and Objectives: Leucocyte depletion improves the quality of stored blood units. We have studied its role on blood viscosity. Materials and Methods: Viscosity of CPDA-1 blood units was measured in a Couette viscometer at shear rates of 94.5 and 0.1 s^–1 prior to and following filtration with the Leukotrap^® A1 system on day 0 and after 21 days at +4°C. Results: On day 0, high but not low shear viscosity was significantly decreased. The red blood cell morphology was unaffected. On day 21, blood viscosity was increased similarly for unfiltered and filtered samples at both shear rates. The echinocytosis observed after storage correlated with the increase in viscosity. Conclusion: Leucocyte depletion is associated with a decrease in high shear viscosity. This effect is, however, completely lost after 21 days.

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Auswirkungen der Leukozytendepletion auf die Lagerungsqualität von Vollblut

Abstract: The Leukotrap A1 whole blood filter system is an interesting option for hospitals lacking the technical capacity to separate whole blood into components. The clinical suitability remains to be investigated by means of clinical studies.

Cited by 5 publications

References 10 publications

The Type of Storage of Autologous Blood Does Not Influence Homologous Transfusion Requirements in Hip Arthroplasty

The Type of Storage of Autologous Blood Does Not Influence Homologous Transfusion Requirements in Hip Arthroplasty

Die Qualität von Vollblut in Abhängigkeit von Herstellungsverfahren (Leukozytendepletion) und Lagerungsdauer. Bedeutung für die operationsvorbereitende Eigenblutspende*

Effects of Leucocyte Depletion on Rheologic Properties of Human CPDA-1 Blood

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