Australia (Hepatitis-Associated) Antigen: Physicochemical And Immunological Characteristics

Recovery of Australia Antigen from Human Plasma Products Separated by a Modified Cohn Fractionation

“…Thus fibrinogen has often been incriminated in this context [6,8,151 at least before it became customary t o subject this fraction to repeated washings during manufacture. Fraction I11 (thrombin) and fraction IV and, The association of the Australia (or SH-) antigen to serum hepatitis is now rather convincingly established (see reviews by LE BOUVIER and MCCOLLUM [13] and b y ZUCKERMAN et al [19]). However, the identity of the infectious agent has n o t yet been settled.…”

The Genome of Hepatitis B Virus

“…Several particulate structures in serum are known to carry HBsAg determinants on their surfaces; the antigen has not been found in a non particulate or low molecular-weight form in serum (4,55). It is well established that the surface of the HBsAg-bearing p�rticles is antigenically complex.…”

Glycoproteins Associated with Hepatitis B Antigen

“…A marked heterogeneity has been described for HB Ag, including variations in morphology, antigenic properties [1] and biophysical and biochemical properties [2], Previous chemical studies of H B Ag have concentrated on the nature of the constitutive polypeptides of purified particles and on the nature of the associated lipids [3.4], PAGE studies from this laboratory using I25l-labeled HB Ag protein revealed six polypeptides with molecular weights ranging from 10,000 to 39,000 [2], By use of Coomassie Brilliant Blue stain for protein, G erin [5] described 3-4 additional proteins ranging in molecular weight from 55,000 to 95,000. The binding of HB Ag by Concanavalin A (Con A) suggested that the antigen contained glycoproteins [6], In this study we have examined the possi bility that some of the HB Ag proteins may be glycosylated.…”