Aurintricarboxylic acid as a nuclease inhibitor in fungal protoplasts

Ramón, Daniel; Ferrer, Sergi; Vicente, Eduardo; Uruburu, F.

doi:10.1111/j.1574-6968.1986.tb01657.x

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…ATA inhibits a variety of enzymes that process nucleic acids, including several RNA polymerases,30•31 reverse transcriptases,32-34 DNA polymerases,32•35-38 and nucleases. [39][40][41][42][43][44][45][46][47] The fact that ATA inhibits these protein-nucleic acid interactions by binding to the protein, not the nucleic acid,35•40 would lead one to suspect that the present cosalane analogues act by binding to IN as opposed to the DNA substrate. At physiological pH, the carboxylate anions present in ATA, cosalane (1), and the cosalane analogues may bind electrostatically to the protonated basic amino acid residues present in the C-terminal, DNA-binding domain of .…”

Section: Discussionmentioning

confidence: 99%

Cosalane Analogs with Enhanced Potencies as Inhibitors of HIV-1 Protease and Integrase

Cushman

Golebiewski²,

Pommier³

et al. 1995

J. Med. Chem.

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Several new analogues of the novel anti-HIV agent cosalane have been synthesized and evaluated as inhibitors of HIV-1 integrase and protease, HIV-1 replication, HIV-1 and HIV-2 cytopathicity, HIV-1- and HIV-2-mediated syncytium formation, and cytopathicity of a variety of human pathogenic viruses. The congeners displayed enhanced potencies relative to cosalane itself as inhibitors of HIV-1 integrase and protease. The two most potent analogues against HIV-1 integrase displayed IC50 values of 2.2 microM, while the three most potent compounds against HIV-1 protease had IC50 values in the 0.35-0.39 microM range. In addition to its activity against HIV-1 and HIV-2 cytopathicity, cosalane inhibited the cytopathic effects of herpes simplex virus-1, herpes simplex virus-2, and human cytomegalovirus at concentrations that were well below the cytotoxic concentrations. Potentially useful antiviral activities were also revealed for some of the new cosalane congeners against influenza virus, Junin virus, and Tacaribe virus.

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Section: Discussionmentioning

confidence: 99%

Cosalane Analogs with Enhanced Potencies as Inhibitors of HIV-1 Protease and Integrase

Cushman

Golebiewski²,

Pommier³

et al. 1995

J. Med. Chem.

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“…The protoplasts were transformed by thawing a frozen suspension and adding a nuclease inhibitor, aurintricarboxylic acid, to a final concentration of 2 mM (24). Vector DNA in 10 ,ul of TE (18) was added to 200 IlI of the protoplast suspension and incubated for 30 min on ice.…”

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confidence: 99%

In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids

1990

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Particular combinations of fungal strains and transformation vectors allow for fungal rearrangement of normally integrative plasmids, resulting in the creation of linear self-replicating plasmids in Fusarium oxysporum. The rearrangement results in the addition of fungal DNA, including telomere consensus sequences, to plasmid termini. The mechanism by which this rearrangement occurs is unclear, but it has similarities to extrachromosomal gene amplification. A DNA fragment which allows for linear autonomous replication upon reintroduction to the fungus was subcloned and sequenced. This DNA sequence contains the repeated telomeric sequence TTAGGG flanked by a region of twofold symmetry consisting primarily of pUC12 DNA. Isolation and identification of this sequence is the first step toward development of vectors that function as artificial chromosomes in filamentous fungi. This sequence was shown to promote autonomous replication and enhance transformation in several strains of F. oxysporum, Nectria haematococca, and Cryphonectria parasitica.

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Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L.

Barandiaran¹,

Pietro

Martín³

1998

Plant Cell Reports

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Aurintricarboxylic acid as a nuclease inhibitor in fungal protoplasts

Abstract: Aurintricarboxylic acid (ATA), a potent nuclease inhibitor, has been shown to prevent foreign DNA degradation in transformation of Penicillium chrysogenum and Saccharomyces cerevisiae. ATA may be useful not only in vitro but also in vivo.

Cited by 5 publications

References 14 publications

Cosalane Analogs with Enhanced Potencies as Inhibitors of HIV-1 Protease and Integrase

Cosalane Analogs with Enhanced Potencies as Inhibitors of HIV-1 Protease and Integrase

In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids

Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L.

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