Abstract:Cell and gene therapies (CGTs) are projected to transform healthcare precision in the biotherapeutics sector. However, for their true potential to be realised, advancements must be made to optimising their manufacture, such that CGT production is precise, reproducible and robust. This includes monitoring and control of complex cell culture conditions, such as extracellular and subcellular biochemical parameters, for which there are no readily available automated analytical systems. Biosensors, such as fluoresc… Show more
“…Current assessment systems are often limited by throughput of the products and many are destructive or sample altering in nature 15. Previous micro-sensory approaches focussed on miniaturising existing sensory elements, such as microelectrodes16 or fibre optic sensors,17 which can cause substantial damage to biological systems.…”
Elucidating the intracellular fate of silica-coated superparamagnetic iron nanoparticles (SiMAGs) using fluorescent pH-sensitive nanosensors, super-resolution fluorescence microscopy and particle counting flow cytometry.
“…Current assessment systems are often limited by throughput of the products and many are destructive or sample altering in nature 15. Previous micro-sensory approaches focussed on miniaturising existing sensory elements, such as microelectrodes16 or fibre optic sensors,17 which can cause substantial damage to biological systems.…”
Elucidating the intracellular fate of silica-coated superparamagnetic iron nanoparticles (SiMAGs) using fluorescent pH-sensitive nanosensors, super-resolution fluorescence microscopy and particle counting flow cytometry.
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