Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells

Harrison, Richard P.; Chauhan, Veeren M.; Onion, David; Aylott, Jonathan W.; Sottile, Virginie

doi:10.1039/c8ra09089k

Cited by 7 publications

(12 citation statements)

References 45 publications

(46 reference statements)

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“…The use of fluorescently labelled MPs has advantages beyond their use for whole body imaging: they are amenable to fluorescent, confocal and super-resolution microscopy [10]. Such particles could be further developed as therapeutic agents, acting both as cell anchors and as cell activators, as their coating or formulation could be functionalised to also provide a biological benefit to host cells [55].…”

Section: Discussionmentioning

confidence: 99%

“…Iron oxide nanoparticles have been developed as contrast agents employed for advanced MRI imaging in vivo [4,5,6], and more recently as theranostics mediators, enabling the application of targeted hyperthermia for tumour treatment [7]. Their cytocompatibility and capacity for efficient cellular uptake also made them applicable to in vitro studies, facilitated by the availability of commercial fluorescently tagged particles compatible with confocal and advanced super-resolution imaging [8,9,10]. Efficient spontaneous uptake of MPIO has been documented in a range of cell types including human stem cells, and was recently shown to be compatible with nanoparticle-based intracellular sensing [10].…”

Section: Introductionmentioning

confidence: 99%

“…Their cytocompatibility and capacity for efficient cellular uptake also made them applicable to in vitro studies, facilitated by the availability of commercial fluorescently tagged particles compatible with confocal and advanced super-resolution imaging [8,9,10]. Efficient spontaneous uptake of MPIO has been documented in a range of cell types including human stem cells, and was recently shown to be compatible with nanoparticle-based intracellular sensing [10]. Beyond their use as contrast agent, internalised iron oxide particles can also confer magneto-responsiveness to cells, thereby providing a means to remotely affect cell behaviour through exposure to magnetic force [11].…”

Section: Introductionmentioning

confidence: 99%

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Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration

et al. 2019

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The success of cell therapy approaches is greatly dependent on the ability to precisely deliver and monitor transplanted stem cell grafts at treated sites. Iron oxide particles, traditionally used in vivo for magnetic resonance imaging (MRI), have been shown to also represent a safe and efficient in vitro labelling agent for mesenchymal stem cells (MSCs). Here, stem cells were labelled with magnetic particles, and their resulting response to magnetic forces was studied using 2D and 3D models. Labelled cells exhibited magnetic responsiveness, which promoted localised retention and patterned cell seeding when exposed to magnet arrangements in vitro. Directed migration was observed in 2D culture when adherent cells were exposed to a magnetic field, and also when cells were seeded into a 3D gel. Finally, a model of cell injection into the rodent leg was used to test the enhanced localised retention of labelled stem cells when applying magnetic forces, using whole body imaging to confirm the potential use of magnetic particles in strategies seeking to better control cell distribution for in vivo cell delivery.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration

et al. 2019

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“…So, in this context, these are superior to gadolinium-based contrast agents [ 28 ]. IONP-labelling of MSCs has been tested in-vitro, and labelled MSCs have been successfully detected by MRI and tested in animal models of disease [ 29 ], where the combination of IONP-labelling of MSCs and MRI has shown huge diagnostic and therapeutic potential [ 30 ]. Therefore, before reinventing the wheel and exploring new approaches, it would be beneficial to revisit the field and assess IONP’s potential in improving MSC detection and homing post-administration.…”

Section: Rationalementioning

confidence: 99%

“…After degradation and metabolism, iron inside these particles is incorporated into the cellular iron pool. Total cellular/tissue iron content is increased, which facilitates detection via MRI [ 27 , 28 , 30 , 33 ] …”

Section: Fundamentals Of Labelling Mscs With Ionpsmentioning

confidence: 99%

Iron Oxide Nanoparticles in Mesenchymal Stem Cell Detection and Therapy

Mehta

2022

Stem Cell Rev and Rep

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Mesenchymal stem cells (MSCs) exhibit regenerative and reparative properties. However, most MSC-related studies remain to be translated for regular clinical usage, partly due to challenges in pre-transplantation cell labelling and post-transplantation cell tracking. Amidst this, there are growing concerns over the toxicity of commonly used gadolinium-based contrast agents that mediate in-vivo cell detection via MRI. This urges to search for equally effective but less toxic alternatives that would facilitate and enhance MSC detection post-administration and provide therapeutic benefits in-vivo. MSCs labelled with iron oxide nanoparticles (IONPs) have shown promising results in-vitro and in-vivo. Thus, it would be useful to revisit these studies before inventing new labelling approaches. Aiming to inform regenerative medicine and augment clinical applications of IONP-labelled MSCs, this review collates and critically evaluates the utility of IONPs in enhancing MSC detection and therapeutics. It explains the rationale, principle, and advantages of labelling MSCs with IONPs, and describes IONP-induced intracellular alterations and consequent cellular manifestations. By exemplifying clinical pathologies, it examines contextual in-vitro, animal, and clinical studies that used IONP-labelled bone marrow-, umbilical cord-, adipose tissue- and dental pulp-derived MSCs. It compiles and discusses studies involving MSC-labelling of IONPs in combinations with carbohydrates (Venofer, ferumoxytol, dextran, glucosamine), non-carbohydrate polymers [poly(L-lysine), poly(lactide-co-glycolide), poly(L-lactide), polydopamine], elements (ruthenium, selenium, gold, zinc), compounds/stains (silica, polyethylene glycol, fluorophore, rhodamine B, DAPI, Prussian blue), DNA, Fibroblast growth Factor-2 and the drug doxorubicin. Furthermore, IONP-labelling of MSC exosomes is reviewed. Also, limitations of IONP-labelling are addressed and methods of tackling those challenges are suggested. Graphical Abstract

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Exploring the future of regenerative medicine: Unveiling the potential of optical microscopy for structural and functional imaging of stem cells

Raju,

Nayak,

Acharya

et al. 2024

Journal of Biophotonics

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Regenerative medicine, which utilizes stem cells for tissue and organ repair, holds immense promise in healthcare. A comprehensive understanding of stem cell characteristics is crucial to unlock their potential. This study explores the pivotal role of optical microscopy in advancing regenerative medicine as a potent tool for stem cell research. Advanced optical microscopy techniques enable an in‐depth examination of stem cell behavior, morphology, and functionality. The review encompasses current optical microscopy, elucidating its capabilities and constraints in stem cell imaging, while also shedding light on emerging technologies for improved stem cell visualization. Optical microscopy, complemented by techniques like fluorescence and multiphoton imaging, enhances our comprehension of stem cell dynamics. The introduction of label‐free imaging facilitates noninvasive, real‐time stem cell monitoring without external dyes or markers. By pushing the boundaries of optical microscopy, researchers reveal the intricate cellular mechanisms underpinning regenerative processes, thereby advancing more effective therapeutic strategies. The current study not only outlines the future of regenerative medicine but also underscores the pivotal role of optical microscopy in both structural and functional stem cell imaging.

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Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells

Abstract: Elucidating the intracellular fate of silica-coated superparamagnetic iron nanoparticles (SiMAGs) using fluorescent pH-sensitive nanosensors, super-resolution fluorescence microscopy and particle counting flow cytometry.

Cited by 7 publications

References 45 publications

Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration

Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration

Iron Oxide Nanoparticles in Mesenchymal Stem Cell Detection and Therapy

Exploring the future of regenerative medicine: Unveiling the potential of optical microscopy for structural and functional imaging of stem cells

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