Augmented cytotoxicity using the physical adsorption of Poloxamer 188 on allicin-loaded gelatin nanoparticles

Abstract

Objectives The aim of this work was to study the effect of the physically adsorbed Poloxamer 188 coating polymer on the cytotoxic activity of allicin-loaded gelatin nanoparticles. Methods The double desolvation method was utilised to prepare the nanoparticles which were characterised for particle size (PS), polydispersity index (PDI) and zeta potential and visualised using transmission electron microscopy. The coating density of the used polymer was determined using 1H-nuclear magnetic resonance (1H-NMR); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to evaluate the cytotoxicity on HepG-2 cell lines. Key findings The particles were spherical possessing a PS of 714 ± 25.21 nm and a PDI of 0.663 ± 0.143. These results together with the 1H-NMR results analysis confirmed the efficient coating of Poloxamer 188. The coating of particles rendered them more cytotoxic, scoring an IC50 of 6.736 µm (2-folds lower than the uncoated counter parts and 4-folds lesser than the allicin solution), and apt for cancer-targeting. Moreover, the prepared nanoparticles were stable to gamma-sterilisation and to a storage of 12 months. Conclusions Augmented cytotoxicity on HepG-2 cell lines was obtained using the physical adsorption of an abundant and relatively cheap material, Poloxamer 188, on allicin-loaded gelatin nanoparticles.

Bioavailability, Health Benefits, and Delivery Systems of Allicin: A Review

“…80 Ossama et al prepared allicin-loaded gelatin nanoparticles with poloxamer 188 coating by a double-desolvation method with a particle size of 714 ± 25.21 nm, a PDI of 0.663 ± 0.143, and an EE of 39.32% ± 1.3. 81 The cytotoxicity against HepG2 cells was also enhanced by 3-fold compared with free unformulated allicin. Hashemy et al prepared allicin-loaded chitosan−egg phospholipid nanoparticles (AC-PLCF-NPs) modified with polyethylene glycol and folic acid by a self-assembling method.…”

“…Nanoparticles are nanoscale materials (1–100 nm) with a high surface area and reactivity that can be employed for food delivery to enhance the bioavailability, functionality, quality, and safety of food products by offering high loading capacity and stability, sustained controlled-release properties, and the ability to transport hydrophilic and lipophilic nutrients across cell membranes and biological barriers. , Fan et al prepared allicin-loaded polylactic acid–glycolic acid nanoparticles by a water–oil–water emulsion method with high encapsulation efficiency (EE) and drug loading, as well as a steady controlled-release rate of 75.0 ± 79.20% in an acidic environment and 14.0 ± 67.44% in a neutral environment . Ossama et al prepared allicin-loaded gelatin nanoparticles with poloxamer 188 coating by a double-desolvation method with a particle size of 714 ± 25.21 nm, a PDI of 0.663 ± 0.143, and an EE of 39.32% ± 1.3 . The cytotoxicity against HepG2 cells was also enhanced by 3-fold compared with free unformulated allicin.…”

PEG 400:Trehalose Coating Enhances Curcumin-Loaded PLGA Nanoparticle Internalization in Neuronal Cells

“…We performed the surface modification of BNPs and CNPs using the classic adsorption method, adjusted from the procedure settled by Ossama et al [ 17 , 28 ]. BNPs or CNPs were resuspended in solutions with increasing equal concentrations of PEG and trehalose (2.5, 6.5, 8.0, and 12.5% v/v and w / v ), respectively, for 12, 24, and 48 h, at room temperature.…”

Combinatorial chemotherapy via poloxamer 188 surface-modified PLGA nanoparticles that traverse the blood-brain-barrier in a glioblastoma model

“…Specifically, poloxamer188 (P188) and polysurbate80 are coatings which have been used on NPs for CNS targeted delivery [16]. P188 is an inexpensive and non-ionic surfactant [17], which enhances the internalization of NPs into cells and allows NPs to transfer across the BBB [18]. In this regard, it has been suggested that P188-coated NPs can adsorb apolipoprotein E and interact with LDL receptors on endothelial cells [18].…”