Augmentation of intramembranous bone in rabbit calvaria using an occlusive barrier in combination with demineralized bone matrix (DBM): A pilot study

Beltrán, Víctor; Engelke, Wilfried; Prieto, Ruth; Valdivia-Gandur, Iván; Navarro, Pablo; Manzanares, María Cristina; Borie, Eduardo; Fuentes, Ramón

doi:10.1016/j.ijsu.2014.03.010

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…This indicated that both materials increased the bone gain in CSBD. Although, these materials restored about 35% of the volume of surgically removed bone, they created an artificial bridge between the bony edges, preventing the ingrowth of tegument connective tissue into defect space and maintained the required volume in CSBD, as observed in ours previous study (Rocha et al, 2014) and by others studies on osteoconductive matrices (Beltrán et al, 2014; Xu et al, 2018). The newly formed bone was in close contact with the DBB and pBCP surface without interposition of connective tissue, demonstrating an excellent osteoconductive property of these materials, as already observed in other studies (Bighetti et al, 2020; Santos et al, 2018).…”

Section: Discussionmentioning

confidence: 56%

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

et al. 2020

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F1-protein fraction (F1) is a natural bioactive compound extracted from the rubber tree, Hevea brasiliensis, and has been recently studied for its therapeutic potential in wound healing. In this study, we investigated the concentration-dependent effects of F1 (0.01%, 0.025%, 0.05%, and 0.1%) incorporated into deproteinized bovine bone (DBB) and porous biphasic calcium phosphate (pBCP), on the repair of rat calvarial critical-size bone defects (CSBD). The defects were analyzed by 3Dmicrotomography and 2D-histomorphometry at 12 weeks postsurgery. The binding efficiency of F1 to pBCP (96.3 ± 1.4%) was higher than that to DBB (67.7 ± 3.3%). In vivo analysis showed a higher bone volume (BV) gain in all defects treated with DBB (except in 0.1% of F1) and pBCP (except in 0.05% and 0.1% of F1) compared to the CSBD without treatment/control group (9.96 ± 2.8 mm 3). DBB plus 0.025% F1 promoted the highest BV gain (29.7 ± 2.2 mm 3 , p < .0001) compared to DBB without F1 and DBB plus 0.01% and 0.1% of F1. In the pBCP group, incorporation of F1 did not promote bone gain when compared to pBCP without F1 (15.9 ± 4.2 mm 3 , p > .05). Additionally, a small BV occurred in defects treated with pBCP plus 0.1% F1 (10.4 ± 1.4 mm 3, p < .05). In conclusion, F1 showed a higher bone formation potential in combination with DBB than with pBCP, in a concentration-dependent manner. Incorporation of 0.25% F1 into DBB showed the best results with respect to bone formation/repair in CSBD. These results suggest that DBB plus 0.25% F1 can be used as a promising bioactive material for application in bone tissue engineering. K E Y W O R D S bone repair, deproteinized bovine bone, natural latex protein fraction, porous biphasic calcium phosphate, protein adsorption, rat calvarial critical-size bone defects 1 | INTRODUCTION Bone defects can heal spontaneously under suitable physiological and environmental conditions due to the bone's ability to repair (Cameron et al., 2013). However, large skeletal defects resulting from malformation, infection, trauma, or tumor resection are challenging problems in contemporary reconstructive surgery because the size of these defects exceed the bone's natural capacity to repair (Henkel et al.,

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Section: Discussionmentioning

confidence: 56%

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

et al. 2020

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“…The use of a matrix or a scaffold [19] into which cells can adhere plays a fundamental role in the regeneration process.…”

Section: Angiogenesis In Regenerationmentioning

confidence: 99%

Application of Bioreactor Concept and Modeling Techniques to Bone Regeneration and Augmentation Treatments

Deccó¹,

Zuchuat²

2016

Advanced Surfaces for Stem Cell Research

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During almost two decades of research in the field of Tissue Engineering and Regenerative Medicine, a variety of biomaterials and techniques have been developed, and a series of results and clinical evidence have been published to support and improve bone regeneration and augmentation processes. The latest techniques include the use of growth factors and platelet-rich plasma preparations in combination with natural or artificial scaffolds, membranes, and stem cells. However, the application of these concepts depends heavily on a favorable microenvironment for the regeneration, which is provided by the use of the bioreactor. The bioreactor is a system in which a microenvironment can be created to promote bone regeneration by providing favorable biomechanical conditions, protecting against infection, and allowing proper nutrition of the regenerating tissue by vascularization. The latest research in this field is very promising as preliminary studies have shown good results concerning bone quality and architecture. In relation to the bioreactor principle, computational modeling represents a very effective tool to predict bone ingrowth. In addition, based on the obtained results, the use of these data to prevent diseases in the human osseous-articular system is expected in the near future.

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“…To overcome these drawbacks, allogeneic or xenogeneic grafts or fillers such as alloplastic bone substitutes were developed and are used in combination with barrier membranes [6,7,8,9]. Generally, traumatic bone lesions involve the breaking of blood vessels, which triggers the coagulation cascade and the formation of a blood clot.…”

Section: Introductionmentioning

confidence: 99%

Bone Augmentation in Rabbit Tibia Using Microfixed Cobalt-Chromium Membranes with Whole Blood and Platelet-Rich Plasma

et al. 2015

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Background: Bone augmentation is a subject of intensive investigation in regenerative bone medicine and constitutes a clinical situation in which autogenous bone grafts or synthetic materials are used to aid new bone formation. Method: Based on a non-critical defect, Co-Cr barrier membranes were placed on six adult Fauve de Bourgogne rabbits, divided into two groups: whole blood and PRP. Three densitometric controls were performed during the experiment. The animals were euthanized at 30, 45, 60, and 110 days. The presence of newly formed bone was observed. Samples for histological studies were taken from the augmentation center. Results: External and internal bone tissue augmentation was observed in almost all cases. Significant differences between PRP- and whole blood–stimulated bone augmentation were not observed. At 60 days, bones with PRP presented higher angiogenesis, which may indicate more proliferation and cellular activity. Conclusion: PRP activates the bone regeneration process under optimized conditions by stimulation of osteoblast proliferation after six weeks, when a significant difference in cellular activity was observed. Membranes could stimulate bone augmentation at the site of placement and in the surrounding areas.

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Augmentation of intramembranous bone in rabbit calvaria using an occlusive barrier in combination with demineralized bone matrix (DBM): A pilot study

Cited by 5 publications

References 23 publications

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

Application of Bioreactor Concept and Modeling Techniques to Bone Regeneration and Augmentation Treatments

Bone Augmentation in Rabbit Tibia Using Microfixed Cobalt-Chromium Membranes with Whole Blood and Platelet-Rich Plasma

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