Augmentation of expression of immunocytes’ functions by seed extract of Ziziphus mauritiana (Lamk.)

Mishra, Tulika; Bhatia, Aruna

doi:10.1016/j.jep.2009.10.033

Cited by 26 publications

(13 citation statements)

References 20 publications

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“…Administration of ZMS at different doses increased the SOD and CAT levels in a dose-dependent manner indicating the protective function of ZMS. In our previous study [14], phytochemical analysis of the extract showed the presence of betulinic acid which is a known anticancer agent [11] and the antitumor and apoptosis inducing property of extract may be in part due to the presence of betulinic acid. It is well established that betulinic acid activates apoptosis independent of the death receptors (CD95/Fas), but induces the successive activation of caspase 9 and caspase 3 [46, 47].…”

Section: Discussionmentioning

confidence: 99%

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Anticancer Potential of Aqueous Ethanol Seed Extract of Ziziphus mauritiana against Cancer Cell Lines and Ehrlich Ascites Carcinoma

Mishra

Khullar

Bhatia

2010

Evidence-Based Complementary and Alternative Medicine

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Ziziphus mauritiana (Lamk.) is a fruit tree that has folkloric implications against many ailments and diseases. In the present study, anticancer potential of seed extract of Ziziphus mauritiana in vitro against different cell lines (HL-60, Molt-4, HeLa, and normal cell line HGF) by MTT assay as well as in vivo against Ehrich ascites carcinoma bearing Swiss albino mice was investigated. The extract was found to markedly inhibit the proliferation of HL-60 cells. Annexin and PI binding of treated HL-60 cells indicated apoptosis induction by extract in a dose-dependent manner. The cell cycle analysis revealed a prominent increase in sub Go population at concentration of 20 μg/ml and above. Agarose gel electrophoresis confirmed DNA fragmentation in HL-60 cells after 3 h incubation with extract. The extract also exhibited potent anticancer potential in vivo. Treatment of Ehrlich ascites carcinoma bearing Swiss albino mice with varied doses (100–800 mg/kg b.wt.) of plant extract significantly reduced tumor volume and viable tumor cell count and improved haemoglobin content, RBC count, mean survival time, tumor inhibition, and percentage life span. The enhanced antioxidant status in extract-treated animals was evident from decline in levels of lipid peroxidation and increased levels of glutathione, catalase, and superoxide dismutase.

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Section: Discussionmentioning

confidence: 99%

“…Plant sample has been kept in Voucher specimen DOB (305) PUP at Punjabi University, Patiala. Aqueous-ethanolic seed extract of Ziziphus mauritiana (ZMS) was prepared and standardized as described earlier [14]. …”

Section: Methodsmentioning

confidence: 99%

Anticancer Potential of Aqueous Ethanol Seed Extract of Ziziphus mauritiana against Cancer Cell Lines and Ehrlich Ascites Carcinoma

Mishra

Khullar

Bhatia

2010

Evidence-Based Complementary and Alternative Medicine

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“…In one case, an oral dose of 500 mg/kg was needed to see an effect in rodents (Sharma and Ray, 1997) equivalent to an intake of 25-50 g intake in humans. In a recent Journal of Ethnopharmacology (JEP) paper, an aqueous-ethanolic seed extract of Ziziphus mauritiana was studied in mice using 100-400 mg/kg of extract administred intraperitonially (Mishra and Bhatia, 2010). Clearly, such dosages are very high.…”

Section: A Matter Of Formulationmentioning

confidence: 99%

Plant immunostimulants—Scientific paradigm or myth?

Gertsch

Viveros–Paredes

Taylor

2011

Journal of Ethnopharmacology

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“…Acute toxicity was studied as described by Mishra and Bhatia [26]. The mice were given 20 to 200 mg of A. nidulans lectin/kg body weight orally and 20 to 200 mg/kg body weight, intraperitoneally.…”

Section: Acute Toxicitymentioning

confidence: 99%

“…Effectiveness of levamisole as an immune modulator is largely known and the present study also compares the immunomodulatory potential of the lectin to that of levamisole [27]. Based on previous work on immunomodulatory effect of levamisole [26,28], the dose of levamisole used was 2.5 mg/kg per day.…”

Section: Immunomodulatory Activity Assaymentioning

confidence: 99%

Immunomodulatory and Therapeutic Potential of a Mycelial Lectin from Aspergillus nidulans

Singh

Bhari

Rana

et al. 2011

Appl Biochem Biotechnol

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Lectins bind to surface receptors on target cells, and activate a cascade of events, eventually leading to altered immune status of host. The immunomodulatory potential of purified lectin from Aspergillus nidulans was evaluated in Swiss albino mice treated intraperitoneally with seven different doses of purified lectin. Lectin prevented BSA-induced Arthus reaction and systemic anaphylaxis. The enhanced functional ability of macrophages was evident from respiratory burst activity and nitric oxide production in splenocyte cultures. Interferon-gamma and interleukin-6 levels were significantly up-regulated in treated groups. Maximum stimulatory effect was observed at the dose of 1.5 mg/kg body weight. Therapeutic potential of A. nidulans lectin was assessed against trinitrobenzene sulfonic acid-induced ulcerative colitis in male Wistar rats. Rats pre-treated with 80 mg/kg body weight of purified lectin intraperitoneally prior to colitis induction showed lesser disease severity and recovery within 7 days, while rats post-treated with the same dose showed recovery in 11 days. The results demonstrate immunomodulatory effects of A. nidulans lectin in Swiss albino mice, resulting in improved immune status of the animals and unfold its curative effect against ulcerative colitis in rat model. This is the first report on immunomodulatory and therapeutic potential of a lectin from microfungi.

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Augmentation of expression of immunocytes’ functions by seed extract of Ziziphus mauritiana (Lamk.)

Cited by 26 publications

References 20 publications

Anticancer Potential of Aqueous Ethanol Seed Extract of Ziziphus mauritiana against Cancer Cell Lines and Ehrlich Ascites Carcinoma

Anticancer Potential of Aqueous Ethanol Seed Extract of Ziziphus mauritiana against Cancer Cell Lines and Ehrlich Ascites Carcinoma

Plant immunostimulants—Scientific paradigm or myth?

Immunomodulatory and Therapeutic Potential of a Mycelial Lectin from Aspergillus nidulans

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