Augmentation of Antibody Responses of Mice to Inhaled Protein Antigens by Simultaneously Inhaled Bacterial Lipopolysaccharides

Mizoguchi, Kenji; Nakashima, Izumi; Hasegawa, Yoshinori; Isobe, K; Nagase, Fumihiko; Kawashima, Kohei; Shimokata, Kaoru; Kato, Naoya

doi:10.1016/s0171-2985(86)80090-0

Cited by 8 publications

(8 citation statements)

References 17 publications

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“…When compared with tracheostomy or direct tracheal puncture (Su et al 2004), this method is faster, less invasive, not mutilating and probably less painful for the animal, thus representing an example of 'refined' airway management. As also recently shown with a radioactive tracer (Su et al 2004), the certainty of dosage and substance application is higher than in most alternatives, including: direct 'blind' transoral intratracheal injection (Vernooy et al 2001); or external surgical tracheal preparation and injection (Kujime & Natelson 1981) or non-surgical injection (Su et al 2004); intranasal application (Szarka et al 1997, Su et al 2004; aerosol chamber exposition (Mizoguchi et al 1986), otoscopic (DeLeonardis et al 1995, Hastings & Summers-Torres 1999, direct (Spoelstra et al 2007) or fibreoptic laryngoscopic (Costa et al 1986), or arthroscopic transoral tracheal application (Vergari et al 2003). The intraoral substance application is also used (Vlad et al 1970).…”

Section: Discussionmentioning

confidence: 92%

Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

et al. 2008

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SummaryAirway access is needed for a number of experimental animal models, and the majority of animal research is based on mouse models. Anatomical conditions in mice are small, and the narrow glottic opening allows intubation only with a subtle technique. We therefore developed a microscopic endotracheal intubation method with a wire guide technique in mice anaesthetized with halothane in oxygen. The mouse is hung perpendicularly with its incisors on a thread fixed on a vertical plate. The tongue is placed with a pair of forceps between the left hand's thumb and forefinger and slightly pulled, while the neck and thorax are positioned using the third and fourth fingers. By doing so, the neck can be slightly stretched, which allows optimal visualization of the larynx and the vocal cords. To ensure a safe intubation, a fine wire guide is placed under vision between the vocal cords and advanced about 5 mm into the trachea. An intravenous 22G Â 1 in. plastic or Teflon catheter is guided over this wire. In a series of 41 mice, between 21 and 38 g, the success rate for the first intubation attempt was .95%. Certainty of the judgement procedure was 100% and success rate was higher using the described method when compared with a transillumination method in a further series. The technique is safe, less invasive than tracheostomy and suitable for controlled ventilation and pulmonary substance application.

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Section: Discussionmentioning

confidence: 92%

Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

et al. 2008

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“…LPS is a major stimulant of the innate immune system through interactions with TLR-4, and it often exhibits potent adjuvant properties (29,34). Previously established immunological mechanisms involved with adjuvant function of LPS include the production of proinflammatory or immunoregulatory cytokines (7), the stimulation of antigen-presenting cells (APCs) to upregulate the expression of costimulatory molecules (6), and the control of dendritic cell migration (38).…”

Section: Discussionmentioning

confidence: 99%

“…, 2004 could also enhance the uptake and presentation of heterologous vaccine antigens to mucosal immune cells. Simultaneously, or in concert with the Ipa protein activity, the LPS component of Invaplex, a previously identified immunostimulator (29), may be capable of binding Toll-like receptor 4 (TLR-4) and stimulating cells of the innate immune system to release proinflammatory cytokines, which in turn influence adaptive immune responses.…”

mentioning

confidence: 99%

Mucosal Adjuvant Properties of theShigellaInvasin Complex

2006

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“…This is because LPS has been shown to act as an adjuvant for allergic contact sensitivity to haptens 40 and to be an adjuvant for immunoglobulin E responses when inhaled with antigen. [41][42][43][44] In addition, LPS acts synergistically with allergen resulting in a magnification of specific allergen-induced mediator release. [45][46][47] Thus, problems of LPS contamination on natural latex products are not only pyrogenicity, but may also be associated with other adverse reactions, such as tissue irritation and induction or enhancement of latex allergy.…”

Section: Discussionmentioning

confidence: 99%

Chemical and biological evaluation of endotoxin contamination on natural rubber latex products

Haishima

Murai

Nakagawa

et al. 2001

J. Biomed. Mater. Res.

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Relationship between pyrogenicity and bacterial endotoxin contamination on latex products was demonstrated by chemical analysis and biological assays. In commercially available latex products' surveillance, water extracts prepared from one surgical glove and two silicone elastomer-coated Foley catheters sterilized by gamma-irradiation were obviously pyrogenic in rabbits. The induced fever was monophasic at low dose of the pyrogenic extracts and biphasic at high dose. These extracts exhibited limulus amebocyte lysate gelation activity, and induced inflammatory cytokine (interleukin-1, interleukin-6, and tumor necrosis factor-alpha) production from MM6-CA8 human monocytoid cells. These biological properties, including pyrogenicity, completely disappeared by treating the pyrogenic extracts with endotoxin-adsorbent affinity column. Limulus amebocyte lysate activity and cytokine production from MM6-CA8 cells induced by the extracts were significantly decreased by endotoxin inhibitors, an active fragment peptide of an 18-kDa cationic antimicrobial protein and a synthetic lipid A B464 analogue. Furthermore, very small amounts of 2-keto-3-deoxyoctonate and 3-hydroxy fatty acid, which are common constituents of bacterial endotoxins, were detected by gas chromatography-mass spectrometry analysis of the pyrogenic extracts. These findings clearly showed that the pyrogenicity found in these latex products originated from endotoxins contaminating the products.

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Augmentation of Antibody Responses of Mice to Inhaled Protein Antigens by Simultaneously Inhaled Bacterial Lipopolysaccharides

Cited by 8 publications

References 17 publications

Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

Mucosal Adjuvant Properties of theShigellaInvasin Complex

Chemical and biological evaluation of endotoxin contamination on natural rubber latex products

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