Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

Hamacher, Jürg; Arras, Margarete; Bootz, Frank; Weiß, Markus; Schramm, R.; Moehrlen, Ueli

doi:10.1258/la.2007.006068

Cited by 42 publications

(35 citation statements)

References 16 publications

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“…The results obtained from the correlation between necessary time to completion of intubation and the weight of the animal corroborates another study (HAMACHER et al, 2008), showing a negative correlation between these variables. Results are readily understood, since the higher the weight of the animal, the greater will be its oral cavity structures, thereby facilitating intubation.…”

Section: New Technique Intubationsupporting

confidence: 87%

“…Other simpler methods, not requiring specific materials, however, require more training and have questionable success rates, as well as injury history in the glottis, larynx and/or vocal cords of animals. Among them can be mentioned: blind intubation (STARK et al, 1981), making a handmade laryngoscope (ABRÃO et al, 1994), use of a specially-developed fiber optic laryngoscope (LINDEN et al, 2000), insertion of wedge in the oral region (JOU et al, 2000), transillumination method (LIZIO et al, 2001), performing of videoendoscopy (KONNO et al, 2014) and use of perpendicular (HAMACHER et al, 2008) or inclined plaque (KASTL et al, 2004;ALZABEN et al, 2009) …”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of lung function of rats using a new intubation technique and tracheostomy

Serra

Brito

Cavalcante

2017

Braz. J. Vet. Res. Anim. Sci.

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Several studies use intubation or tracheostomy for data collection in lung function of rats. Due to the difficulty of performing intubation, tracheostomy is most commonly used. Knowing this difficulty, this paper demonstrates a new way to perform the intubation technique and compares pulmonary function variables obtained using intubation or tracheostomy. For the analysis of our new technique of intubation, 30 rats were used, and the comparative analysis of lung function data obtained through the use of our technique of intubation and tracheostomy, 16 rats were used, divided into two groups. The T group underwent tracheostomy. The IT group underwent initial intubation and, awaiting recovery, a week later was again subjected to intubation group, which was then called IT-1W. Our intubation technique is performed with the aid of a wedge, inclined bed and percutaneous transillumination. No animals died during the intubation procedure, and about 77% of the animals were intubated at the first attempt. We did not obtain statistically significant differences in the pulmonary function variables between the groups. Our intubation technique is easily learned and reproduced. The implications of such a technique can be generalized to all laboratories dealing with intubation of rats.Keywords: Intubation. Tracheostomy. Lung function. ResumoVários estudos utilizam a intubação orotraqueal ou traqueostomia para coleta de dados na função pulmonar de ratos. Devido à dificuldade de realização da intubação, a traqueostomia é mais comumente utilizada. Conhecendo esta dificuldade, este trabalho demonstra uma nova maneira de realizar a técnica de intubação e compara as variáveis de função pulmonar obtidas por intubação ou traqueostomia. Para a análise de nossa nova técnica de intubação, foram utilizados 30 ratos, e para a análise comparativa dos dados da função pulmonar obtidos pelo uso de nossa técnica de intubação e traqueotomia, 16 ratos foram utilizados, divididos em dois grupos. O grupo T foi submetido a traqueostomia. O grupo de IT foi submetido inicialmente a intubação e, esperada sua recuperação, uma semana depois foi novamente submetido a intubação, que foi então chamado de grupo IT-1W. Nossa técnica de intubação é realizada com a ajuda de uma cunha, cama inclinada e transiluminação percutânea. Nenhum animal morreu durante o procedimento de intubação, e cerca de 77% dos animais foram intubados na primeira tentativa. Não foram obtidas diferenças estatisticamente significativas nas variáveis de função pulmonar entre os grupos. Nossa técnica de intubação é facilmente aprendida e reproduzida. As implicações de tal técnica podem ser generalizadas para todos os laboratórios que lidam com a intubação de ratos.Palavras-chave: Intubação. Traqueostomia. Função pulmonar. | 2Braz.

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Section: New Technique Intubationsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Comparative analysis of lung function of rats using a new intubation technique and tracheostomy

Serra

Brito

Cavalcante

2017

Braz. J. Vet. Res. Anim. Sci.

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“…Increasing this pull slightly will straighten the visualization path so the vocal cords can be seen. Hamacher, et al, described a unique intubation system with microscopic visualization 4 . Their online video of this intubation is excellent and very instructive, although the means of positioning the head and neck is not entirely clear from the video and figure.…”

Section: Discussionmentioning

confidence: 99%

“…Such a technique has considerable utility in doing repeat pulmonary function or bronchoalveolar lavage in individual mice in longitudinal studies 2 . Since that original paper, there have been several other papers that have described different approaches to mouse intubation [3][4][5][6][7][8][9] . While all of these methods can be used successfully, they usually require considerable training or cost.…”

Section: Introductionmentioning

confidence: 99%

A Simple Method of Mouse Lung Intubation

Das

MacDonald

Chang

et al. 2013

JoVE

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A simple procedure to intubate mice for pulmonary function measurements would have several advantages in longitudinal studies with limited numbers or expensive animal. One of the reasons that this is not done more routinely is that it is relatively difficult, despite there being several published studies that describe ways to achieve it. In this paper we demonstrate a procedure that eliminates one of the major hurdles associated with this intubation, that of visualizing the trachea during the entire time of intubation. The approach uses a 0.5 mm fiberoptic light source that serves as an introducer to direct the intubation cannula into the mouse trachea. We show that it is possible to use this procedure to measure lung mechanics in individual mice over a time course of at least several weeks. The technique can be set up with relatively little expense and expertise, and it can be routinely accomplished with relatively little training. This should make it possible for any laboratory to routinely carry out this intubation, thereby allowing longitudinal studies in individual mice, thereby minimizing the number of mice needed and increasing the statistical power by using each mouse as its own control. Video LinkThe video component of this article can be found at

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“…Since that original paper, there have been several other papers that have described different approaches to mouse intubation [1][2][3][4][5][6][7][8][9] . While all of these methods can be used successfully, they usually require considerable training, and are often not without a nontrivial failure rate.…”

Section: Introductionmentioning

confidence: 99%

Instillation and Fixation Methods Useful in Mouse Lung Cancer Research

Limjunyawong

Mock²,

Mitzner

2015

JoVE

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The ability to instill live agents, cells, or chemicals directly into the lung without injuring or killing the mice is an important tool in lung cancer research. Although there are a number of methods that have been published showing how to intubate mice for pulmonary function measurements, none are without potential problems for rapid tracheal instillation in large cohorts of mice. In the present paper, a simple and quick method is described that enables an investigator to carry out such instillations in an efficient manner. The method does not require any special tools or lighting and can be learned with very little practice. It involves anesthetizing a mouse, making a small incision in the neck to visualize the trachea, and then inserting an intravenous catheter directly. The small incision is quickly closed with tissue adhesive, and the mice are allowed to recover. A skilled student or technician can do instillations at an average rate of 2 min/mouse. Once the cancer is established, there is frequently a need for quantitative histologic analysis of the lungs. Traditionally pathologists usually do not bother to standardize lung inflation during fixation, and analyses are often based on a scoring system that can be quite subjective. While this may sometime be sufficiently adequate for gross estimates of the size of a lung tumor, any proper stereological quantification of lung structure or cells requires a reproducible fixation procedure and subsequent lung volume measurement. Here we describe simple reliable procedures for both fixing the lungs under pressure and then accurately measuring the fixed lung volume. The only requirement is a laboratory balance that is accurate over a range of 1 mg-300 g. The procedures presented here thus could greatly improve the ability to create, treat, and analyze lung cancers in mice. Video LinkThe video component of this article can be found at

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Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination

Cited by 42 publications

References 16 publications

Comparative analysis of lung function of rats using a new intubation technique and tracheostomy

Comparative analysis of lung function of rats using a new intubation technique and tracheostomy

A Simple Method of Mouse Lung Intubation

Instillation and Fixation Methods Useful in Mouse Lung Cancer Research

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