Augmentation and starvation of calcium, magnesium, phosphate on lipid production of Scenedesmus obliquus

Esakkimuthu, Sivakumar; Krishnamurthy, Venkatesan; Govindarajan, Ramkumar; Swaminathan, K.

doi:10.1016/j.biombioe.2016.03.019

Cited by 54 publications

(27 citation statements)

References 41 publications

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“…Nitrogen is a critical element in the formation of proteins, lipids, and nucleic acids (Guschina and Harwood, 2013), while phosphate is essential for the synthesis of ATP, phospholipids, and the backbone of DNA and RNA (Andersen, 2013). In our studies, both S. obliquus and C. vulgaris showed a complete consumption of nitrogen and a partial consumption of phosphorus in Bold and Bristol culture media (particularly faster consumption by S. obliquus), which favored a rapid accumulation of lipids (Esakkimuthu et al, 2016;Kiran et al, 2016;Lari et al, 2016). Prasad and coworkers (2018) indicated that the uptake of phosphorus and nitrogen is linked.…”

Section: Discussionmentioning

confidence: 51%

“…Micronutrients play a critical role in the metabolic functions of algae, since these elements present in the media act as co-enzymes or energy carriers (Juneja et al, 2013). A concentration of calcium and magnesium below 50 mg/l was shown to promote lipid accumulation in S. obliquus, although the biomass concentration was not significantly affected (Esakkimuthu et al, 2016). Similarly, in the present study, the growth of S. obliquus in the AAP medium with low concentrations of calcium and magnesium (Table 1) resulted in the highest percentage of lipid accumulation, compared to the percentages obtained with the algae cultured on Bristol and Bold media.…”

Section: Discussionmentioning

confidence: 99%

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Towards the production of microalgae biofuels: the effect of the culture medium on lipid deposition

García¹,

Rangel-Basto²,

Barajas-Solano³

et al. 2019

bta

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Microalgae have been studied in recent years for various reasons, one among them being their potential in the production of biofuels. Biofuels are considered as a renewable source that does not compete with the food industry, and their production results in a reduced environmental footprint. The objective of the present study was to evaluate the different culture conditions that influence the productivity of the microalgae Chlorella vulgaris and Scenedesmus obliquus as a potential source for the production of biodiesel. Bold, Bristol, and modified Algal Assay Procedure (AAP) culture media were evaluated to determine which of them can provide the best conditions that favor biomass production and lipid deposition. The results indicated that S. obliquus cultivated in Bold medium exhibited a higher production of biomass after 30 days (1.730 g/l), while C. vulgaris showed a higher production in the modified AAP medium (1.880 g/l). Although the cultures grown in the AAP medium showed a more significant percentage of biomass comprising lipids than those in the other two media, between the two microalgae, the highest final lipid production was obtained by growing S. obliquus in Bold medium (0.685 g/l, 39.60% of biomass). N, P, Fe, and Mo were the elements added to the growth media, and depending on their concentration in the media, there were differences in the production of lipids. Thus, it can be concluded that S. obliquus cultivated in Bold medium has the highest potential in the production of lipids for biodiesel, but it is necessary to further optimize the cultivation of biomass to obtain a greater quantity of lipids, while interfering as little as possible in its synthesis capacity.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Towards the production of microalgae biofuels: the effect of the culture medium on lipid deposition

García¹,

Rangel-Basto²,

Barajas-Solano³

et al. 2019

bta

View full text Add to dashboard Cite

show abstract

“…The inefficiency of Soxhlet extraction was also reported by other studies. 21 The least efficient solvent for extraction was chloroform, indicating that most of the lipids present in the biomass of S. ecornis are best extracted by intermediate polarity in nature. Esakkimuthu et al 22 reported the use of chloroform-methanol (2:1) mixture as being more efficient in extracting total lipids from Scenedesmus obliquus microalgae.…”

Section: Resultsmentioning

confidence: 99%

FATTY ACID ETHYL ESTERS FROM MICROALGAE OF Scenedesmus ecornis BY ENZYMATIC AND ACID CATALYSIS

Almeida¹,

Araújo²,

Florentino³

et al. 2017

Quim. Nova

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Microalgae are an indispensable food source for the various growth stages of mollusks, crustaceans, and several fish species. Using a microalgae biomass present in the Amazonian ecosystem (Macapá-AP), we study extraction methods for fatty acid such as solvent extraction (magnetic stirring and/or Soxhlet) and/or hydrolysis (acid and/or enzymatic catalysis) followed by esterification and/or direct transesterification. Extraction of crude triacylglycerides by mechanical stirring at room temperature was more efficient than continuous reflux (Soxhlet). Subsequently, the lipid extract was subject to transesterification with ethanol and CAL-B as a biocatalyst, leading to production of fatty acid ethyl esters (FAEE). Additionally, FAEEs were prepared by hydrolysis of crude triacylglycerides followed by acid-mediated esterification or enzymatic catalysis (lipase). In this case, the type of catalyst did not significantly influence FAEE yields. In the lipid extract, we identified palmitic, linoleic, oleic, and stearic acids with palmitic acid being the most abundant. Our results suggest that enzymatic catalysis is a viable method for the extraction of lipids in the microalga, Scenedesmus ecornis.

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“…They play a significant role in growth of microalgae as a cofactor of some key enzymes in the metabolic pathway [35]. The researchers put forward that the intracellular chlorophyll content decreased in the absence or lack of magnesium, which resulted in a negative impact upon photosynthesis amount in Chlorella sp.…”

Section: Resultsmentioning

confidence: 99%

Increasing the Protein Amount of Chlorella vulgaris [Chlorphyta] Strains Isolated from Different Fresh Water Ponds

Duygu¹

2017

JESE-B

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Chlorella vulgaris is a single-cell, spherical green algae and one of the microalgae on which many applied studies are conducted. In the present study, five strains displaying fast and efficient reproduction were chosen among 11 C. vulgaris strains isolated from different fresh water ponds and their cell numbers and the amount of chlorophyll a, protein, lipid, cellulose and carbohydrate were examined. The main goal of the study is to investigate increasing the biochemical contents especially the protein content of C. vulgaris strains in different mediums. In the present study, cell densities were determined through cell count for five days. In parallel with cell count, their chlorophyll a content was determined. The highest cell density was observed with C. vulgaris TOH (Tourism and Hotel Management Pond) strain as 5.5 × 10 4 h/mL, and the chlorophyll a content as 4.3 × 10 2 mg/m 3 . The highest intracellular protein amount was determined with C. vulgaris GUH (Gazi University Rectorship Pond) (0.061 g/100 mL) and the highest lipid amount was attained with C. vulgaris UIK (Ulus Construction Well) strain as 0.019 g/100 mL. The process of increasing the intracellular protein amount in C. vulgaris GUH strain was carried out in Prat, Yagojinski and Chlorella medium. The results indicated that Chlorella medium increased the intracellular protein amount.

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Augmentation and starvation of calcium, magnesium, phosphate on lipid production of Scenedesmus obliquus

Cited by 54 publications

References 41 publications

Towards the production of microalgae biofuels: the effect of the culture medium on lipid deposition

Towards the production of microalgae biofuels: the effect of the culture medium on lipid deposition

FATTY ACID ETHYL ESTERS FROM MICROALGAE OF Scenedesmus ecornis BY ENZYMATIC AND ACID CATALYSIS

Increasing the Protein Amount of Chlorella vulgaris [Chlorphyta] Strains Isolated from Different Fresh Water Ponds

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