Atypical immature cervical metaplasia: immunoprofiling and longitudinal outcome☆

Duggan, Máire A.; Akbari, Majid; Magliocco, Anthony M.

doi:10.1016/j.humpath.2006.05.013

Cited by 26 publications

(19 citation statements)

References 19 publications

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Section: Discussionmentioning

confidence: 99%

“…Previous studies have reported conflicting results about the likelihood of progression of AIM [27][28][29][30] . Based on p16 and Ki67 immunohistochemical profiles, some authors have demonstrated that AIM is more similar to LSIL than HSIL [27] . Along this line of reasoning, a recent work by our group has found a low frequency of aberrant expression of certain cell-cycle proteins (p16, cyclin D1, Ki67, p53 and ProEx C) thus confirming previous observations [31] .…”

Section: Discussionmentioning

confidence: 99%

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Human Papillomavirus Genotyping in Histological Sections of Precursor Lesions of Cervical Carcinoma: Its Role as a Possible Adjunct for the Evaluation of the Oncogenic Potential of Specific Human Papillomavirus Genotypes – A Study in a Coastal Region of Southeastern Spain

Doménech-Peris

Conesa‐Zamora²,

Sahuquillo-Frias³

et al. 2010

Gynecol Obstet Invest

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Background: Human papillomavirus (HPV) genotyping is usually performed on cytological specimens with the aim of discerning between high- and low-risk genotypes. Methods: Paraffin-embedded sections (n = 241) comprising 16 normal/benign (N/B) cervical sections, 72 low-grade squamous intraepithelial lesions (LSIL), 133 high-grade SIL (HSIL), 6 invasive carcinomas (cervical cancer), and 14 atypical immature metaplasias (AIMs) were DNA extracted and HPV genotyped. Results: The most frequent HPV genotypes found were 16 and 58. HPV16 was detected in 0% N/B, 18.1% LSIL, 42.9% HSIL (p < 0.001), 50% carcinoma, and 35.7% AIM, whilst HPV58 was detected in 25.0, 20.8, 16.5, 0 and 35.7% of these lesions, respectively. Discussion: The high prevalence of HPV58 and the low prevalence of HPV18 suggest the limited effectiveness of HPV vaccination in southeast Spain (prevention of 45.1% HSILs). The HPV genotype distribution profile in AIM suggests that these lesions are more similar to LSIL than HSIL pointing to a low risk of progression to cervical cancer. These results reinforce the necessity of assessing the specific genotype rather than distinguishing between high- or low-risk HPV. The use of histological section instead of cytological specimens for specific HPV genotyping would be very useful in order to ascertain the oncogenic potential of each of the genotypes found in a given area.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus Genotyping in Histological Sections of Precursor Lesions of Cervical Carcinoma: Its Role as a Possible Adjunct for the Evaluation of the Oncogenic Potential of Specific Human Papillomavirus Genotypes – A Study in a Coastal Region of Southeastern Spain

Doménech-Peris

Conesa‐Zamora²,

Sahuquillo-Frias³

et al. 2010

Gynecol Obstet Invest

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“…However, the small size of biopsy specimens, potential sampling error, and heterogeneous distribution of dysplastic lesions can result in over-and under-diagnosis. Another difficulty is the existence of several CIN mimics such as immature squamous metaplasia [6,7]. Several biomarkers suitable for use on paraffin sections have been established to avoid these diagnostic inaccuracies.…”

Section: Introductionmentioning

confidence: 99%

Biomarker expression in cervical intraepithelial neoplasia: potential progression predictive factors for low-grade lesions

Ozaki¹,

Zen²,

Inoue³

2011

Human Pathology

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Abbreviations: CIN, cervical intraepithelial neoplasia; HPV, human papilloma virus; p16, p16INK4a; MCM2, minichromosome maintenance protein 2; TPO2A, DNA topoisomerase IIHChybridcapture II: LSIL, low-grade squamous intraepithelial lesion; HSIL, high-grade squamous intraepithelial lesion.  Key words: Cervical cancer; p16; ProEx C; HPV; carcinogenesis.Running title: ProEx C and p16 expressions in CIN SummaryThe aim of this study was to reveal whether three biomarkers (p16, ProEx C, and HPV DNA) are useful in the diagnosis of cervical intraepithelial neoplasia and whether they could predict disease progression of cervical intraepithelial neoplasia-1.We analyzed 252 cervical specimens: non-dysplastic mucosa (n=9), cervical intraepithelial neoplasia (n=229), and squamous cell carcinoma (n=14). Immunostaining for p16 and ProEx C, and the hybridcapture II assay for HPV DNA were performed.Expression of p16 and staining for ProEx C were significantly higher in intraepithelial neoplasia-2/3 (96% to 100%) than in non-dysplastic mucosa (11%) or intraepithelial neoplasia-1 (40% to 53%). HPV DNA was detected in 69% of intraepithelial neoplasia-1, 95% of intraepithelial neoplasia-2, and 100% of intraepithelial neoplasia-3.Of 99 patients with intraepithelial neoplasia-1 for whom follow-up data was available, 62 (73%) showed spontaneous regression, 17 (20%) demonstrated persistent low-grade lesion, and 7 (7%) progressed to intraepithelial neoplasia-2/3. Expressions of p16 and staining with ProEx C were significantly higher in the progression group than in the regression group. Testing for p16 and ProEx C were sensitive (86%) and moderately specific (60% and 61%, respectively) in predicting the progression of cervical intraepithelial neoplasia-1. HPV DNA testing was highly sensitive (100%) but less specific (37%). In conclusion, this study revealed that p16 and ProEx C are useful biomarkers for the diagnosis of cervical intraepithelial neoplasia, and have potential as predictors of progression of low-grade lesions.

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“…7,15,25,26 The use of a cocktail of both p16INK4a and Ki-67 antibodies along with the H&E staining has been shown in various studies to aid the proper evaluation of cervical squamous intraepithelial lesions and other benign lesions and for possible prognostication of the progression of a low grade to a high grade squamous intraepithelial neoplasia. 27,28 In this study, only a few cases with low p16INK4a positivity (5.2%) showed Ki-67 positivity but there was a significant correlation between p16INK4a…”

Section: P16ink4a and Ki-67mentioning

confidence: 47%

P16/INK4a AND KI-67 EVALUATION OF INTRAEPITHELIAL AND BENIGN CERVICAL LESIONS AT THE UNIVERSITY COLLEGE HOSPITAL, IBADAN - A RETROSPECTIVE IMMUNOHISTOCHEMICAL STUDY

E¹,

Okolo

et al. 2017

JCGB

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ObjectiveP16INK4a and Ki-67 are adjuncts to current histological assessment of cervical biopsies in identifying cases that require strict follow up and prompt intervention. This study aimed to evaluate P16INK4a and Ki-67 expression in squamous intraepithelial and other benign cervical lesions. MethodsA retrospective cross-sectional study of 153 cases of cervical biopsies diagnosed as CIN and benign cervical lesions between 2006 and 2013 at the University College Hospital, Ibadan, Nigeria. Slides and tissue blocks of all the selected cases were retrieved and classified using the 2003 WHO classification for intraepithelial and benign cervical lesions and were stained with p16INK4a and Ki-67 immunohistochemical stains following heat -induced antigen retrieval. Results were evaluated and compared with histologic diagnosis. ResultsCases were classified as chronic cervicitis (12.3%), squamous Metaplasia (0.7%), CIN 1 (47.1%), CIN 2 (36.6%) and CIN 3 (3.3%). Majority of the non-dysplastic cervical lesions (including chronic cervicitis cases) showed low P16INK4a reactivity. Positive P16INK4a reactivity was seen in 80% of CIN 3 cases, 83.9% of CIN 2 cases, and, surprisingly, in 97.2% of CIN 1 cases. Ki-67 positivity was seen in 36.6% of cases (75% CIN 2 and 60% CIN 3). There was a significant correlation between the H&E diagnoses of CIN and P16INK4a/Ki-67 immunoreactivities. ConclusionMajority of the CIN 1 cases showing low grade p16INK4a immune reactivity strongly suggesting that cervical squamous intraepithelial neoplasia in this environment is likely associated with high grade HPV subtype infections and may predict possible progression to high grade squamous intraepithelial neoplasia. The use of P16INK4a and Ki-67 in the evaluation of cervical biopsies for benign mimics of high grade intraepithelial lesion will aid proper single Pathologist evaluation and help in patients triaging for follow up.

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Atypical immature cervical metaplasia: immunoprofiling and longitudinal outcome☆

Cited by 26 publications

References 19 publications

Human Papillomavirus Genotyping in Histological Sections of Precursor Lesions of Cervical Carcinoma: Its Role as a Possible Adjunct for the Evaluation of the Oncogenic Potential of Specific Human Papillomavirus Genotypes – A Study in a Coastal Region of Southeastern Spain

Human Papillomavirus Genotyping in Histological Sections of Precursor Lesions of Cervical Carcinoma: Its Role as a Possible Adjunct for the Evaluation of the Oncogenic Potential of Specific Human Papillomavirus Genotypes – A Study in a Coastal Region of Southeastern Spain

Biomarker expression in cervical intraepithelial neoplasia: potential progression predictive factors for low-grade lesions

P16/INK4a AND KI-67 EVALUATION OF INTRAEPITHELIAL AND BENIGN CERVICAL LESIONS AT THE UNIVERSITY COLLEGE HOSPITAL, IBADAN - A RETROSPECTIVE IMMUNOHISTOCHEMICAL STUDY

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