Atypical flavobacteria recovered from diseased fish in the Western United States

Heckman, Taylor I.; Yazdi, Zeinab; Pomaranski, Eric K.; Sebastião, Fernanda de Alexandre; Mukkatira, Kaveramma; Vuglar, Brent M.; Cain, Kenneth D.; Loch, Thomas P.; Soto, Esteban

doi:10.3389/fcimb.2023.1149032

Cited by 4 publications

(2 citation statements)

References 106 publications

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“…Histological analysis of the five killed rainbow trout revealed that the spleen presented lymphoid hyperplasia in three and two samples obtained from bath (Figure 2) and intraperitoneal injection challenged fish, respectively. The score of these five fish was two Heckman et al, 2023;Nhinh et al, 2023;Zamora, Fernandez-Garayzábla, et al, 2012;.…”

Section: Challenge Assaymentioning

confidence: 99%

Phenotypic and genomic characterization of a non‐pathogenic Epilithonimonas ginsengisoli isolated from diseased farmed rainbow trout (Oncorhynchus mykiss) in Chile

Miranda,

Irgang,

Concha

et al. 2023

Journal of Fish Diseases

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Flavobacterial infection associated with diseased fish is caused by multiple bacterial species within the family Flavobacteriaceae. In the present study, the Chilean isolate FP99, from the gills of a diseased, farmed rainbow trout (Oncorhynchus mykiss), was characterized using phenotypic and genomic analyses. Additionally assessed was pathogenic activity. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that isolate FP99 belonged to the genus Epilithonimonas, an average nucleotide identity value of 100% was detected with the Chilean isolate identified as Epilithonimonas sp. FP211‐J200. In silico genome analysis, mechanisms for toxins production, and superantigens, adhesion, or other genes associated with virulence were not detected. However, genes encoding proteins for antibiotic resistance were found, including the chrA gene and the nucleotide sequence that encodes for multiple antibiotic resistance MarC proteins. Furthermore, the blaESP‐1 gene (87.85% aminoacidic sequence identity), encoding an extended‐spectrum subclass B3 metallo‐β‐lactamase and conferring carbapenem‐hydrolysing activity, and the tet(X) gene, which encodes a monooxygenase that catalyses the degradation of tetracycline‐class antimicrobials were carried by this isolate. Phenotyping analyses also supported assignment as E. ginsengisoli. Challenge trials against healthy rainbow trout resulted in no observed pathogenic effect. Our findings identify for the first time the species E. ginsengisoli as associated with fish farming, suggesting that this isolate may be a component of the microbiota of the freshwater system. Notwithstanding, poor environmental conditions and any stressors associated with aquaculture situations or lesions caused by other pathogenic bacteria, such as F. psychrophilum, could favour the entry of E. ginsengisoli into rainbow trout.

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Section: Challenge Assaymentioning

confidence: 99%

Phenotypic and genomic characterization of a non‐pathogenic Epilithonimonas ginsengisoli isolated from diseased farmed rainbow trout (Oncorhynchus mykiss) in Chile

Miranda,

Irgang,

Concha

et al. 2023

Journal of Fish Diseases

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show abstract

“…35 (14). W ostatnich latach opisano także kilka nowych gatunków Flavobacterium, jak: F. plurextorum, F. tructae, F. collinsii, F. oncorhynchi i F. piscis, które zostały wyizolowane od narybku pstrąga tęczowego, mającego objawy kliniczne podobne do tych występujących przy zakażeniu F. psychrophilum (16). By móc wykorzystać metodę MALDI-TOF MS do odróżniania tych bakterii, Perez-Sancho i wsp.…”

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Detection of bacterial fish pathogens by MALDI-TOF MS- state of the art

OLECH,

PAŹDZIOR

2024

Medycyna Weterynaryjna

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A key element in the diagnosis of bacterial infections in fish is the rapid identification of the etiological agent of the disease and drug resistance testing. Traditional methods of identifying the bacteria that cause fish diseases are often slow and/or expensive. MALDI-TOF MS is a well-established, rapid, accurate and cost-effective method based on protein profile analysis for the identification of bacterial pathogens in human and veterinary laboratories, and has now found application in the diagnosis of bacterial infections in fish. The method identifies microorganisms based on their protein profile, which is then compared with reference spectra stored in databases. Since not all bacterial fish pathogens are included in commercial databases, libraries are usually created locally for the relevant reference strains/species. As studies have shown, MALDI-TOF MS is suitable for the specific identification of many bacterial fish pathogens enabling rapid diagnosis of infections.

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Rice straw ash and amphibian health: A deep dive into microbiota changes and potential ecological consequences

Dong,

Xu,

Yang

et al. 2024

Science of The Total Environment

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Atypical flavobacteria recovered from diseased fish in the Western United States

Cited by 4 publications

References 106 publications

Phenotypic and genomic characterization of a non‐pathogenic Epilithonimonas ginsengisoli isolated from diseased farmed rainbow trout (Oncorhynchus mykiss) in Chile

Phenotypic and genomic characterization of a non‐pathogenic Epilithonimonas ginsengisoli isolated from diseased farmed rainbow trout (Oncorhynchus mykiss) in Chile

Detection of bacterial fish pathogens by MALDI-TOF MS- state of the art

Rice straw ash and amphibian health: A deep dive into microbiota changes and potential ecological consequences

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