Atypical cells in the normal guinea pig organ of Corti as revealed by micromanipuiation in SEM

M, Reiss

doi:10.1002/jemt.1070200309

Cited by 7 publications

(2 citation statements)

References 28 publications

(24 reference statements)

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“…Retrieved screws from 1, 3, and 6 days (three specimens/surface/time point) were fixed with a modified Karnovsky solution (2% paraformaldehyde, 2.5% glutaraldehyde in 0.05 M sodium cacodylate) (pH 7.4) for 4 h. Specimens were then rinsed with sodium cacodylate buffer and subsequently impregnated with a conductive, metallic layer of osmium, using a modified osmium‐thiocarbohydrazide‐osmium technique (OTOTO) 25. Specimens were then dehydrated in graded series of ethanol and dried with hexamethyldizilasane for 2 × 5 min.…”

Section: Methodsmentioning

confidence: 99%

Effect of mixing conditions on mechanical properties of polylactide/montmorillonite clay nanocomposites

Jollands

Gupta

2010

J of Applied Polymer Sci

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Biopolymer nanocomposites are of great interest to the packaging industry as they can overcome the inferior properties of biopolymers compared to synthetic polymers.However studies on property improvement have been inconclusive about optimum filler levels and mixing conditions. This paper reports on a systematic study of effect on mechanical properties of varying melt mixing conditions and filler level in PLA organoclay composites. Samples were mixed in a batch mixer at various temperatures, times and speeds, at three filler levels. Tensile properties were measured and morphology characterized using small angle X-ray scattering (SAXS). An empirical model was used to predict the optimum mixing conditions. Samples produced at those conditions had the highest modulus of all samples, 66% higher than the average. Samples with different filler levels were made at the optimum conditions and tested for modulus and morphology. The maximum modulus, 40% higher than natural PLA, was achieved at 4wt% filler level, and above that level modulus decreased, similar to findings in some studies but contrary to others. SAXS measurements indicated the samples had a similar intercalated morphology, so the excellent modulus results may 1 Corresponding author. Tel: +61 (3) 9925 2089. Fax: +61 (3) 9925 3746. Email address: Margaret.jollands@rmit.edu.au Jollands, M and Gupta, R 2010, 'Effect of mixing conditions on mechanical properties of polylactide/montmorillonite clay nanocomposites', Journal of Applied Polymer Science, vol. 118, no. 3, pp. 1489-1493 be attributed to presence of smaller tactoids and absence of agglomerates rather than exfoliation.

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Section: Methodsmentioning

confidence: 99%

Effect of mixing conditions on mechanical properties of polylactide/montmorillonite clay nanocomposites

Jollands

Gupta

2010

J of Applied Polymer Sci

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“…The cylinders with attached cells were immersed in 2.5% glutaraldehyde in 0.05 M sodium cacodylate buffer with pH 7.4. Specimens were subsequently rinsed with sodium cacodylate buffer and impregnated with a conductive layer of osmium, using a modified osmiumthiocarbohy-drazide-osmium technique (Reiss, 1992). Dehydratation was performed in graded series of ethanol prior to drying with hexamethyldizilasane for 2 × 5 min.…”

Section: Electron Microscopymentioning

confidence: 99%

In vitro and in vivo evaluation of an injectable premixed calcium phosphate cement; cell viability and immunological response from rat

Åberg

Henriksson

Engqvist

et al. 2011

IJNBM

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By using premixed calcium phosphate cement (CPC) the handling properties of the cement are drastically improved, which is a challenge for traditional injectable CPC. In this article, a premixed acidic CPC has been compared to a conventional water mixed brushite cement to evaluate whether the premixed concept affects the biological response. The cements were evaluated regarding the pH-variation in simulated body fluid (SBF). Further, the biocompatibility of the cement with human mesenchymal stem cells (MSC) was studied in vitro and eventual inflammation properties studied in vivo, after subcutaneous material injections in rats.A larger pH decrease was seen for the conventional cement than the premixed cement in SBF. For both materials, > 90% of the MSC remained alive in vitro. No systemic or macroscopic inflammation was detected, only a mild microscopic inflammation was detected around both materials.In addition to the handling benefit of premixed cements compared to conventional water mixed CPC, the premixed CPC in the present study demonstrated high and in comparison to conventional CPC comparable biocompability, both in vitro and in vivo.

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In vivo gene expression in response to anodically oxidized versus machined titanium implants

Omar

Svensson

Zoric

et al. 2009

J Biomedical Materials Res

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A quantitative polymerase chain reaction technique (qPCR) in combination with scanning electron microscopy was applied for the evaluation of early gene expression response and cellular reactions close to titanium implants. Anodically oxidized and machined titanium miniscrews were inserted in rat tibiae. After 1, 3, and 6 days the implants were unscrewed and the surrounding bone was retrieved using trephines. Both the implants and bone were analyzed with qPCR. A greater amount of cells, as indicated with higher expression of 18S, was detected on the oxidized surface after 1 and 6 days. Significantly higher osteocalcin (at day 6), alkaline phosphatase (at days 3 and 6), and cathepsin K (at day 3) expression was demonstrated for the oxidized surface. Higher expression of tumor necrosis factor-alpha (at day 1) and interleukin-1beta (at days 1 and 6) was detected on the machined surfaces. SEM revealed a higher amount of mesenchymal-like cells on the oxidized surface. The results show that the rapid recruitment of mesenchymal cells, the rapid triggering of gene expression crucial for bone remodeling and the transient nature of inflammation, constitute biological mechanisms for osseointegration, and high implant stability associated with anodically oxidized implants.

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Atypical cells in the normal guinea pig organ of Corti as revealed by micromanipuiation in SEM

Cited by 7 publications

References 28 publications

Effect of mixing conditions on mechanical properties of polylactide/montmorillonite clay nanocomposites

Effect of mixing conditions on mechanical properties of polylactide/montmorillonite clay nanocomposites

In vitro and in vivo evaluation of an injectable premixed calcium phosphate cement; cell viability and immunological response from rat

In vivo gene expression in response to anodically oxidized versus machined titanium implants

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