Attenuation of Theileria lestoquardi infected cells and immunization of sheep against malignant ovine theileriosis

Ahmed, B.M.; Taha, Khalid M.; Enan, Khalid A; Elfahal, A. M.; Hussein, Abdel Rahim M. El

doi:10.1016/j.vaccine.2013.08.004

Cited by 10 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While the use of an attenuated T . lestoquardi -infected cell line has shown protection in one study [12], a more targeted approach to vaccination, ideally utilising antigens conserved between different strains of T . lestoquardi , has practical advantages in terms of safety, production, storage and administration.…”

Section: Discussionmentioning

confidence: 99%

“…[5, 6] and, as with most vector-borne diseases, control of transmission is challenging [7]. In addition, current disease control methods are limited to treatment with a theilericidal compound, buparvaquone [8, 9], and in some countries, vaccination with attenuated parasitized cell lines [10–12]. These methods are not easy to apply successfully.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

et al. 2016

View full text Add to dashboard Cite

As part of an international effort to develop vaccines for Theileria lestoquardi, we undertook a limited screen to test T. lestoquardi orthologues of antigens recognised by CD8+ T lymphocyte responses against T. annulata and T. parva in cattle. Five MHC defined sheep were immunized by live T. lestoquardi infection and their CD8+ T lymphocyte responses determined. Thirteen T. lestoquardi orthologues of T. parva and T. annulata genes, previously shown to be targets of CD8+ T lymphocyte responses of immune cattle, were expressed in autologous fibroblasts and screened for T cell recognition using an IFNγ assay. Genes encoding T. lestoquardi antigens Tl8 (putative cysteine proteinase, 349 aa) or Tl9 (hypothetical secreted protein, 293 aa) were recognise by T cells from one animal that displayed a unique MHC class I genotype. Antigenic 9-mer peptide epitopes of Tl8 and Tl9 were identified through peptide scans using CD8+ T cells from the responding animal. These experiments identify the first T. lestoquardi antigens recognised by CD8+ T cell responses linked to specific MHC class I alleles.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Malignant ovine theileriosis ( T. lestoquardi infection) is an economically important disease infecting small ruminants (Ahmed et al., ). The geographic distribution of T. lestoquardi in the world is limited to five arid countries (Sudan, Tanzania, Iraq, Iran and Oman) (Schnittger et al., ; Shayan et al., ; Zaeemi et al., ; Ahmed et al., ; Renneker et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…This piroplasm would represent a real constraint to the development of small ruminants breeding in endemic regions where the molecular prevalence can reach 100% (Tageldin et al., ; El Ghali and El Hussein, ; Ahmed et al., ). In Africa, this piroplasm has exclusively been reported in northern Sudan (Ahmed et al., ) and Tanzania (Schnittger et al., ). We report herein for the first time a molecular identification and genetic characterization of T. lestoquardi in Maghreb region.…”

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

First Molecular Identification and Genetic Characterization ofTheileria lestoquardiin Sheep of the Maghreb Region

Rjeibi

Darghouth

Rekik³

et al. 2014

Transbound Emerg Dis

View full text Add to dashboard Cite

Theileria lestoquardi is the most prominent Theileria species in small ruminants that causes malignant theileriosis of sheep in Africa and Asia. In the present survey, blood samples and ticks were collected in Kebili (southern Tunisia) from 166 Queue Fine de l'Ouest sheep. Giemsa-stained blood smears, immunofluorescent antibody test (IFAT) and PCR were performed. The DNA was extracted from blood and analysed by PCR targeting 18S rRNA gene of Theileria spp. and then sequenced. A total number of 140 ticks were collected from a total number of 166 sheep during the four seasons. The ticks belonged to two genera and 4 species; the most frequent tick was Hyalomma excavatum 84.3% (118/140) and then Rhipicephalus spp. 15.7% (22/140). Only two animals had positive Giemsa-stained blood smears, and they were also positive by IFAT. The amplicons had 99.3 and 99.6% homology with the BLAST published T. lestoquardi amplicons. To our knowledge, this is the first report of T. lestoquardi in small ruminants within the Maghreb region.

show abstract

Blood, Lymph, and Immune Systems

2022

Goat Medicine

View full text Add to dashboard Cite

Attenuation of Theileria lestoquardi infected cells and immunization of sheep against malignant ovine theileriosis

Cited by 10 publications

References 17 publications

Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells

First Molecular Identification and Genetic Characterization ofTheileria lestoquardiin Sheep of the Maghreb Region

Blood, Lymph, and Immune Systems

Contact Info

Product

Resources

About