Attenuation of The
            <i>In Vivo</i>
            Toxicity of Biomaterials by Polydopamine Surface Modification

Hong, Seonki; Kim, Keum Yeon; Wook, Hwang Jin; Park, Soo Jung; Lee, Kang Dae; Lee, Dong Yun; Lee, Haeshin

doi:10.2217/nnm.11.76

Cited by 267 publications

(208 citation statements)

References 39 publications

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“…Since mussel adhesive protein contains a significant amount of 3,4-dihydroxy-L-phenylalanine (DOPA) and lysine (Lys), 9 catecholamines that contain the key chemical functionalities of DOPA and Lys have been identified as minimalist adhesive protein mimics; it was determined that catecholamines can functionalize a wide range of materials in a simple manner, resulting in biocompatible surfaces. [10][11][12][13][14][15] For example, incubation of solid substrates in an alkaline dopamine solution resulted in polydopamine-coated substrates, and the coating has been widely utilized to prepare the functional surfaces such as, biomolecule-conjugated or mineral-deposited surfaces.…”

mentioning

confidence: 99%

Surface Modification of Highly Ordered Pyrolytic Graphite (HOPG) by a Mussel-Inspired Poly(norepinephrine) Coating: Characterizations and Cell Adhesion Test

Kang

Lee²

2013

Bulletin of the Korean Chemical Society

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mentioning

confidence: 99%

Surface Modification of Highly Ordered Pyrolytic Graphite (HOPG) by a Mussel-Inspired Poly(norepinephrine) Coating: Characterizations and Cell Adhesion Test

Kang

Lee²

2013

Bulletin of the Korean Chemical Society

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“…Recently, the use of polydopamine was found to be safe for use in vivo and can even reduced cytotoxicity. 31 These results suggest that CoCr-PDOP-BMP-7 peptide substrates were not cytotoxic to fibroblasts, and the immobilized peptide on its surface can reduced the attachment and proliferation of fibroblasts.…”

Section: Discussionmentioning

confidence: 74%

Anti‐fibrosis effect of BMP‐7 peptide functionalization on cobalt chromium alloy

Tan

Poh

Cai

et al. 2013

Journal Orthopaedic Research

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Orthopedic metallic prosthetic implants are commonly made of cobalt chromium (CoCr) alloys. However, such metal-based implants are susceptible to fibrous capsule formation on the implant surface after implantation. At the bone-implant interface, this capsule can prevent implant integration, resulting in loosening and failure. Minimizing the development of such a capsule on the CoCr surface would improve direct bone-implant bonding leading to long-term implant functionality. We evaluated the anti-fibrosis effect of bone morphogenic protein-7 (BMP-7) peptide covalently bonded to CoCr alloy. This peptide, a biomimetic derivation of the knuckle epitope of BMP-7, was conjugated at the N-terminus with a cysteine amino acid. X-ray photoelectron spectroscopy (XPS) and probe binding assay were used to evaluate different stages of grafting and surface functionalization using polydopamine coating. Cellular functions were studied using fibroblast attachment, cell proliferation, and MTT assays. Fibroblasts were grown on functionalized and pristine CoCr substrates, and the efficacy of BMP-7 peptide on anti-fibrosis was analyzed via gene expression and protein expression of fibrosis markers ACTA2, Collagen 1A1, and fibronectin. The peptide functionalized substrates showed significant reduction of fibrosis markers expression after 1 week of incubation compared to controls. BMP-7 signaling pathway activation was shown by the presence of phosphorylation of Smad1/5/8. These findings may contribute to the improvement of CoCr implants in orthopedic surgery applications.

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“…However, the chemical substances used for this purpose, such as, APTES, EDAC, and NHS, are harmful to the human body [45]. In this study, titanium was coated with heparin using a dopamine surface modification technique, which has been reported to be biocompatible in in vivo toxicity studies [46]. Dopamine is a small molecule substance that possesses catechol and amine functional groups [47].…”

Section: Discussionmentioning

confidence: 99%

Effects of Immobilizations of rhBMP-2 and/or rhPDGF-BB on Titanium Implant Surfaces on Osseointegration and Bone Regeneration

et al. 2017

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Abstract:The aim of this study was to examine the effects of immobilizing rhPDGF-BB plus rhBMP-2 on heparinized-Ti implants on in vivo osseointegration and vertical bone regeneration at alveolar ridges. Successful immobilizations of rhPDGF-BB and/or rhBMP-2 onto heparinized-Ti (Hepa/Ti) were confirmed by in vitro analysis, and both growth factors were found to be sustained release. To evaluate bone regeneration, rhPDGF-BB, and/or rhBMP-2-immobilized Hepa/Ti implants were inserted into beagle dogs; implant stability quotients (ISQ), bone mineral densities, bone volumes, osseointegration, and bone formation were assessed by micro CT and histometrically. In vivo study showed that the osseointegration and bone formation were greater in the rhPDGF-BB/rhBMP-2-immobilized Hepa/Ti group than in the rhPDGF-BB-immobilized Hepa/Ti group. The rhPDGF-BB/rhBMP-2 immobilized Hepa/Ti group also showed better implant stability and greater bone volume around defect areas and intra-thread bone density (ITBD) than the rhBMP-2-immobilized Hepa/Ti group. However, no significant differences were observed between these two groups. Through these results, we conclude rhBMP-2 immobilized, heparin-grafted implants appear to offer a suitable delivery system that enhances new bone formation in defect areas around implants. However, we failed to observe the synergetic effects for the rhBMP-2 and rhPDGF-BB combination.

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Attenuation of The In Vivo Toxicity of Biomaterials by Polydopamine Surface Modification

Abstract: Our results indicate that polydopamine provides a versatile platform that can reduce the in vivo toxicity of biomaterials that contact tissue or blood.

Cited by 267 publications

References 39 publications

Surface Modification of Highly Ordered Pyrolytic Graphite (HOPG) by a Mussel-Inspired Poly(norepinephrine) Coating: Characterizations and Cell Adhesion Test

Surface Modification of Highly Ordered Pyrolytic Graphite (HOPG) by a Mussel-Inspired Poly(norepinephrine) Coating: Characterizations and Cell Adhesion Test

Anti‐fibrosis effect of BMP‐7 peptide functionalization on cobalt chromium alloy

Effects of Immobilizations of rhBMP-2 and/or rhPDGF-BB on Titanium Implant Surfaces on Osseointegration and Bone Regeneration

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