Anti‐fibrosis effect of BMP‐7 peptide functionalization on cobalt chromium alloy

Tan, Hark Chuan; Poh, Chye Khoon; Cai, Yanli; Wang, Wilson

doi:10.1002/jor.22313

Cited by 18 publications

(17 citation statements)

References 38 publications

(45 reference statements)

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“…Yang et al ( 28 ) demonstrated that BMP-7 inhibited silica-induced pulmonary fibrosis in rats by upregulating the expression of p-Smad1/5/8. In addition, p-Smad1/5/8 was demonstrated to be involved in BMP-7 signaling pathway-induced anti-fibrotic effects on fibrous capsule formation on the surface of orthopedic metallic prosthetic implants ( 29 ). Furthermore, increased expression of p-Smad1/5/8 was observed in mediating the anti-fibrosis effects of BMP-7 in the cornea ( 30 ) and lung ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway

Guo

Lin

Shao

et al. 2017

Molecular Medicine Reports

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Scarring is the inevitable consequence of wound repair, which may cause significant physical and mental pain to patients when excessive. Bone morphogenetic protein-7 (BMP-7) has been proved to inhibit TGF-β-induced fibrosis in various tissues including dermal papilla cells. However, the effect of BMP-7 on hypertrophic scarring, a common proliferative disorder of dermal fibroblasts, has not been determined. To overcome this problem, the present study established a mouse model of thermal injury to investigate the inhibitory effects of BMP-7 on scar formation. The histological analysis of scar tissues was performed by H&E and Masson's trichrome staining. Western blot assay was used to determine the level changes of related proteins and TUNEL assay was performed to assess the apoptosis of scar tissues. The results demonstrated that BMP-7 promoted wound healing and inhibited scar formation when compared with untreated mice. Collagen deposition and the expression of fibrotic proteins were suppressed in the scar tissues of mice treated with BMP-7. In addition, BMP-7 induced fibroblast apoptosis in scar tissues. Furthermore, activation of the BMP-7/Smad1/5/8 signaling pathway may have been involved in the inhibitory effects of BMP-7 on scar formation. In conclusion, the results of the present study indicate that BMP-7 may inhibit excessive scar formation via activation of the BMP-7/Smad1/5/8 signaling pathway. The results present a potential alternative therapeutic strategy for the treatment of hypertrophic scarring.

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Section: Discussionmentioning

confidence: 99%

BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway

Guo

Lin

Shao

et al. 2017

Molecular Medicine Reports

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“…Efforts to improve the bioactivity of CoCrMo have included chemical vapor deposition coating, 17 sol-gel coating, 18 ultraviolet photofunctionalization, 19,20 and immobilization with bone morphogenic peptides. [21][22][23] Surface modification of CoCrMo through topographical methods remains largely un-investigated.…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stem cell response to topographically modified CoCrMo

Logan

Bozec

Traynor

et al. 2015

J Biomedical Materials Res

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Surface roughness on implant materials has been shown to be highly influential on the behavior of osteogenic cells. Four surface topographies were engineered on cobalt chromium molybdenum (CoCrMo) in order to examine this influence on human mesenchymal stem cells (MSC). These treatments were smooth polished (SMO), acid etched (AE) using HCl 7.4% and H2SO4 76% followed by HNO3 30%, sand blasted, and acid etched using either 50 μm Al2O3 (SLA50) or 250 μm Al2O3 grit (SLA250). Characterization of the surfaces included energy dispersive X‐ray analysis (EDX), contact angle, and surface roughness analysis. Human MSCs were cultured onto the four CoCrMo substrates and markers of cell attachment, retention, proliferation, cytotoxicity, and osteogenic differentiation were studied. Residual aluminum was observed on both SLA surfaces although this appeared to be more widely spread on SLA50, whilst SLA250 was shown to have the roughest topography with an R a value greater than 1 μm. All substrates were shown to be largely non‐cytotoxic although both SLA surfaces were shown to reduce cell attachment, whilst SLA50 also delayed cell proliferation. In contrast, SLA250 stimulated a good rate of proliferation resulting in the largest cell population by day 21. In addition, SLA250 stimulated enhanced cell retention, calcium deposition, and hydroxyapatite formation compared to SMO (p < 0.05). The enhanced response stimulated by SLA250 surface modification may prove advantageous for increasing the bioactivity of implants formed of CoCrMo. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3747–3756, 2015.

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“…Then, they were washed with copious amounts water. Washed Ti substrates were coated with polydopamine (Ti-pDOP) as previously described [ 48 ]. In brief, Ti substrates were immersed overnight in dopamine solution (2 mg/mL of dopamine hydrochloride in 10 mM of Tris Buffer at pH = 8.5).…”

Section: Methodsmentioning

confidence: 99%

In Vitro Findings of Titanium Functionalized with Estradiol via Polydopamine Adlayer

Steffi

Shi

Kong

et al. 2017

JFB

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To improve orthopedic implant fixation and reduce post-operative complications, osteogenic molecules are delivered locally by immobilizing them on the surface of implants, which will modulate the biology of cell attachment and differentiation on the implant surface. Estradiol, a natural steroid hormone, maintains bone metabolism by decreasing bone resorption. It either directly or indirectly affects osteoclasts. In this work, estradiol was immobilized on a titanium surface by polydopamine adlayer. Immobilization of estradiol was confirmed by X-ray electron spectroscopy (XPS), immunofluorescence staining and enzyme-linked immunosorbent assay (ELISA). Estradiol-modified substrates enhanced alkaline phosphatases activity (ALP) and calcium deposition of osteoblasts. However, these substrates did not decrease tartrate-resistant acid phosphatase (TRAP) activity and actin ring formation of the osteoclast. The scanning electron microscopic (SEM) images of estradiol-modified substrates showed the formation of estradiol crystals, which decreased the potency of immobilized estradiol. Despite having a successful immobilization of estradiol via the polydopamine technique, the bioavailability and potency of coated estradiol is reduced due to crystallization, suggesting that this is not a suitable system for localized estradiol delivery as tested in vitro here. Consequently, other suitable platforms have to be explored for immobilizing estradiol that will prevent crystal formation while preserving the biological activity.

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Anti‐fibrosis effect of BMP‐7 peptide functionalization on cobalt chromium alloy

Cited by 18 publications

References 38 publications

BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway

BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway

Mesenchymal stem cell response to topographically modified CoCrMo

In Vitro Findings of Titanium Functionalized with Estradiol via Polydopamine Adlayer

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